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AB231229

Anti-MGAM antibody

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(1 Publication)

Rabbit Polyclonal MGAM antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MGAM aa 200-400.

View Alternative Names

MGA, MGAML, MGAM, Maltase-glucoamylase

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGAM antibody (AB231229)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGAM antibody (AB231229)

Formalin-fixed, paraffin-embedded human kidney tissue stained for MGAM using ab231229 at 20 mg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-MGAM antibody (AB231229)
  • WB

Supplier Data

Western blot - Anti-MGAM antibody (AB231229)

All lanes:

Western blot - Anti-MGAM antibody (ab231229) at 3 µg

All lanes:

Recombinant human MGAM

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 210 kDa,75 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGAM antibody (AB231229)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGAM antibody (AB231229)

Formalin-fixed, paraffin-embedded human kidney tissue stained for MGAM using ab231229 at 20 mg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-MGAM antibody (AB231229)
  • WB

Supplier Data

Western blot - Anti-MGAM antibody (AB231229)

All lanes:

Western blot - Anti-MGAM antibody (ab231229) at 3 µg/mL

All lanes:

Human serum

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 210 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MGAM aa 200-400. The exact immunogen used to generate this antibody is proprietary information.

O43451

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGAM or maltase-glucoamylase is an important enzyme in carbohydrate digestion. This protein with a mass of approximately 185 kDa functions primarily in the small intestine where it acts on oligosaccharides breaking them down into glucose units. MGAM exhibits substrate specificity cleaving alpha-14-glycosidic linkages and assisting in the final steps of starch digestion. The enzyme exists in brush-border membranes and demonstrates similar activity to another enzyme sucrase-isomaltase.
Biological function summary

Maltase-glucoamylase plays a significant role in nutrient absorption by facilitating glucose release which is necessary for energy production. It associates with the brush-border membrane in enterocytes aiding digestive processes. It does not typically form part of a larger protein complex but works in tandem with other digestive enzymes. Its activity ensures efficient breakdown of carbohydrates influencing nutritional uptake and metabolic balance.

Pathways

Maltase-glucoamylase participates in the starch and sucrose metabolism pathway. Its enzymatic action represents a final step in this digestive pathway leading to the absorption of glucose the primary energy source. MGAM works alongside sucrase-isomaltase which also contributes to breaking down dietary carbohydrates into absorbable monosaccharides. This coordinated activity ensures effective carbohydrate digestion and nutrient assimilation.

Maltase-glucoamylase's function can affect conditions such as carbohydrate malabsorption and type 2 diabetes. Deficiencies or abnormalities in MGAM activity can lead to compromised carbohydrate digestion resulting in gastrointestinal symptoms. The enzyme's role in glucose release also ties it to metabolic conditions as improper glucose management influences the onset of type 2 diabetes. Interaction with proteins such as insulin can modulate these effects indicating the enzyme's significance in metabolic health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Alpha-(1,4) exo-glucosidase involved in breakdown of dietary starch oligosaccharides in small intestine. Cleaves the non-reducing alpha-(1,4)-linked glucose residue in linear dextrins with retention of anomeric center stereochemistry (PubMed : 12547908, PubMed : 18036614, PubMed : 18356321, PubMed : 22058037, PubMed : 27480812). Mainly hydrolyzes short length oligomaltoses having two to seven glucose residues (PubMed : 12547908, PubMed : 18036614, PubMed : 18356321, PubMed : 22058037, PubMed : 27480812). Can cleave alpha-(1,2), alpha-(1,3) and alpha-(1,6) glycosidic linkages with lower efficiency, whereas beta glycosidic linkages are usually not hydrolyzed (PubMed : 27480812).
See full target information MGAM
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical and translational hepatology 12:496-504 PubMed38779522

2024

The Therapeutic Effects of Baicalein on the Hepatopulmonary Syndrome in the Rat Model of Chronic Common Bile Duct Ligation.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyang Zeng,Yuhao Lei,Chunyong Yang,Xianfeng Wu,Liang Zhang,Zhiyong Yang,Lin Chen,Xiaobo Wang,Karine Belguise,Yujie Li,Bin Yi
View all publications
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