Anti-MGAT1 antibody [EPR14247]

• WB

Supplier Data

Western blot - Anti-MGAT1 antibody [EPR14247] (AB180578)

All lanes:

Western blot - Anti-MGAT1 antibody [EPR14247] (ab180578) at 1/10000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 51 kDa

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• WB

Supplier Data

Western blot - Anti-MGAT1 antibody [EPR14247] (AB180578)

All lanes:

Western blot - Anti-MGAT1 antibody [EPR14247] (ab180578) at 1/2000 dilution

All lanes:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit igG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 51 kDa

false

• WB

CiteAb

Western blot - Anti-MGAT1 antibody [EPR14247] (AB180578)

MGAT1 western blot using anti-MGAT1 antibody [EPR14247] ab180578. Publication image and figure legend from Fisher, P., Spencer, H., et al., 2019, Cell Rep, PubMed 31018136.

ab180578 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab180578 please see the product overview.

Predicting Organizational Changes in Trafficking Defective Cell Lines(A and B) Observed and simulated glycan profiles of Cog4KD HeLa cell lines (A) and Cog4KO HEK293T cell lines (B). Error bars for glycan profiling are SEM for n = 3.(C) Total effective enzymatic rate changes predicted by the model following fitting as a result of Cog4 perturbation. The x axis is on a log scale. Error bars for total effective enzymatic rates and distributions are SD for n = 20 (Cog4KD HeLa) and n = 21 (Cog4KO HEK293T) individual fitting procedures.(D) Western blot analysis of endogenous MGAT1, GalT, and FUT8 and quantification normalized to WT HEK293T. Error bars for western blot quantification are SD for n = 3. ns, not significant; *p < 0.05 for a Student's t test.(E) Predicted relative distribution of MAN1 following the fitting of WT and Cog4KD HeLa glycan profiles.(F) Predicted relative distributions of selected enzymes in WT and Cog4KO HEK293T cells in each cisterna normalized to the total predicted effective enzymatic rate for each enzyme for each cell line.(G) Airyscan confocal microscopy of GM130 and exogenous GalT-YFP in nocodazole-treated WT and Cog4KO HEK293T cell lines.(H) Pearson's correlation coefficient for WT and Cog4KO HEK293T cells. Pearson's correlation coefficients were calculated for each Golgi stack, and error bars are SD for n = 4 cells with 151 (WT) and 131 (Cog4KO) stacks. ***p < 0.001 for a Student's t test.See also Figures S3 and S6 and Table S3.

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