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AB250228

Anti-MGAT1 antibody [EPR14247] - BSA and Azide free

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Rabbit Recombinant Monoclonal MGAT1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

GGNT1, GLCT1, GLYT1, MGAT, MGAT1, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I, GNT-I, GlcNAc-T I

2 Images
Western blot - Anti-MGAT1 antibody [EPR14247] - BSA and Azide free (AB250228)
  • WB

Supplier Data

Western blot - Anti-MGAT1 antibody [EPR14247] - BSA and Azide free (AB250228)

This data was developed using ab180578, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MGAT1 antibody [EPR14247] (<a href='/en-us/products/primary-antibodies/mgat1-antibody-epr14247-ab180578'>ab180578</a>) at 1/10000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 51 kDa

false

Western blot - Anti-MGAT1 antibody [EPR14247] - BSA and Azide free (AB250228)
  • WB

Supplier Data

Western blot - Anti-MGAT1 antibody [EPR14247] - BSA and Azide free (AB250228)

This data was developed using ab180578, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MGAT1 antibody [EPR14247] (<a href='/en-us/products/primary-antibodies/mgat1-antibody-epr14247-ab180578'>ab180578</a>) at 1/2000 dilution

All lanes:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit igG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14247

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250228 is the carrier-free version of ab180578.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGAT1 also known as mannosyl (α-13-)-glycoprotein β-12-N-acetylglucosaminyltransferase is an enzyme with a molecular mass of approximately 51 kDa. It is a critical component of the N-glycosylation process in eukaryotic cells. This protein resides primarily in the Golgi apparatus where it acts as a glycosyltransferase enzyme. MGAT1 catalyzes the transfer of N-acetylglucosamine to the mannose residues on the glycan chain which is an important step in forming complex N-glycans. This modification impacts several cellular functions.
Biological function summary

MGAT1 plays an essential role in protein folding stability and function by modifying N-glycans attached to nascent proteins. The enzyme acts as part of a complex that includes other enzymes like MGAT2 MGAT3 and MGAT5 in the glycan biosynthesis pathway. MGAT1's activity significantly influences cell-cell communication signaling and immune responses. Its function enhances the diversity and biological roles of glycoproteins within cells impacting various physiological processes.

Pathways

MGAT1 is a significant player in the protein glycosylation and folding pathways. It interacts with proteins like MGAT2 and galectin-3 indicating its involvement in broader metabolic processes. N-glycan biosynthesis pathway is its primary pathway where it contributes by enabling the elongation and branching of N-glycan structures. This glycosylation process affects cellular processes such as signal transduction and protein trafficking through downstream effects on glycoproteins.

MGAT1 has implications in congenital disorders of glycosylation and cancer. Abnormal MGAT1 activity leads to improper glycosylation causing congenital disorders that affect multiple systems including nervous and digestive systems. In cancer MGAT1-linked glycan alterations on surface proteins contribute to tumor progression and metastasis. Disrupted interactions with proteins like MGAT5 can increase cell surface glycoprotein complexity promoting cancer cell survival and immune evasion.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
See full target information MGAT1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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