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AB177206

Anti-MGAT4B antibody

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(1 Publication)

Rabbit Polyclonal MGAT4B antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MGAT4B aa 50-250.

View Alternative Names

UNQ906/PRO1927, MGAT4B, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb, GlcNAc-T IVb, GnT-IVb, N-acetylglucosaminyltransferase IVb

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGAT4B antibody (AB177206)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGAT4B antibody (AB177206)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human kidney tissue labeling MGAT4B with ab177206 at 1/100 dilution.

Western blot - Anti-MGAT4B antibody (AB177206)
  • WB

Supplier Data

Western blot - Anti-MGAT4B antibody (AB177206)

All lanes:

Western blot - Anti-MGAT4B antibody (ab177206) at 1/500 dilution

Lane 1:

HepG2 cell lysate

Lane 2:

Human fetal kidney lysate

Predicted band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MGAT4B aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

Q9UQ53

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGAT4B also known as beta-14-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase plays a critical role in glycoprotein biosynthesis. This enzyme has a molecular mass of approximately 54 kDa and is responsible for adding N-acetylglucosamine in a beta-14 linkage to the core mannose residues in N-glycans. You can find MGAT4B expressed in various tissues especially in the liver and pancreas where it contributes to complex carbohydrate formation on glycoproteins.
Biological function summary

MGAT4B influences N-glycan branching significantly impacting cell signaling and cell-cell interactions. It functions as a part of an enzyme family that is essential for the glycosylation of proteins adding diversity to the glycan structures. Such activity contributes to various biological processes including cellular communication and immune response. While the protein does not form a stable complex with other proteins it interacts with substrates and cofactors vital for its enzymatic function.

Pathways

MGAT4B plays an important role in the N-glycan biosynthesis pathway. This pathway influences key aspects of cellular development and immune system modulation. MGAT4B acts alongside proteins like MGAT5 in these pathways contributing to the synthesis of highly branched glycans that modify protein stability and function. This activity ties into larger glycosylation networks vital for maintaining cellular homeostasis.

MGAT4B links to cancers and immunological disorders. Abnormal expression of MGAT4B may lead to altered glycosylation patterns often observed in tumorigenesis where it plays a role alongside proteins like MGAT3. By affecting immune cell signaling and surface glycan composition MGAT4B also intertwines with disorders such as lupus showing how its dysregulation can impact immune homeostasis and host defense mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glycosyltransferase that catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains (PubMed : 10372966, PubMed : 17006639). Prefers complex-type N-glycans over hybrid-types (PubMed : 17006639). Has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor in N-glycan biosynthesis (PubMed : 17006639).
See full target information MGAT4B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:325 PubMed40619404

2025

The scaffold protein DLG4 facilitates RNF63-mediated ubiquitination and degradation of STAT3 in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shisong Chen,Hongjie Xu,Ning Li,Yang Yang,Ruxi Pang,Shuwei Zhang,Junjie Qiao,Hao Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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