Anti-MGAT5 antibody [3E9]

• Flow Cyt

Unknown

Flow Cytometry - Anti-MGAT5 antibody [3E9] (AB87977)

Overlay histogram showing Jurkat cells stained with ab87977 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87977, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This image was generated using the ascites version of the product.

• WB

Unknown

Western blot - Anti-MGAT5 antibody [3E9] (AB87977)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-MGAT5 antibody [3E9] (ab87977) at 1 µg/mL

All lanes:

jurkat cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 85 kDa

false

• WB

AbReview51060****

Western blot - Anti-MGAT5 antibody [3E9] (AB87977)

Blocking was via 5% milk in PBST incubated for 1 hour at 22°C

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-MGAT5 antibody [3E9] (ab87977) at 1/2000 dilution

All lanes:

Human RWPE prostate cells at 20 µg

Secondary

All lanes:

Donkey anti-mouse polyclonal HRP conjugate at 1/10000 dilution

Predicted band size: 85 kDa

Observed band size: 55 kDa,95 kDa

true

Exposure time: 40s

Image is courtesy of an AbReview submitted by Armen Petrosyan.

• WB

Unknown

Western blot - Anti-MGAT5 antibody [3E9] (AB87977)

The recombinant protein used as the immunogen has a predicted size of 37 kDa.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-MGAT5 antibody [3E9] (ab87977) at 1 µg/mL

All lanes:

recombinant protein fragment with a proprietary tag (the immunogen) at 0.1 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Predicted band size: 85 kDa

Observed band size: 37 kDa

false