AB68522

Anti-MGEA5/OGA antibody

Name: Anti-MGEA5/OGA antibody (ab68522) | Abcam
Brand: Abcam Limited
SKU: AB68522
Price: 535 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(4 Publications)

Mouse Polyclonal MGEA5/OGA antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human OGA.

View Alternative Names

HEXC, KIAA0679, MEA5, MGEA5, OGA, Protein O-GlcNAcase, Beta-N-acetylglucosaminidase, Beta-N-acetylhexosaminidase, Beta-hexosaminidase, Meningioma-expressed antigen 5, N-acetyl-beta-D-glucosaminidase, N-acetyl-beta-glucosaminidase, Nuclear cytoplasmic O-GlcNAcase and acetyltransferase, NCOAT

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human OGA.

O60502

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGEA5 also referred to as OGA or O-GlcNAcase serves an important mechanical role in cellular metabolism. This enzyme with a molecular mass of around 103 kDa is primarily involved in the removal of O-GlcNAc modifications from serine/threonine residues on nuclear and cytoplasmic proteins. It is expressed in various tissues but exhibits predominant activity in the brain liver and pancreas. Alternative names like "OGA stains" or "OGA b" may also be encountered in literature or research contexts.

Biological function summary

MGEA5 acts as an important modulator of protein function and regulation by cycling O-GlcNAc modifications thereby influencing processes like transcription signal transduction and apoptosis. While not part of a well-defined complex it frequently interacts with other proteins involved in glucose metabolism and the cellular stress response. Its activity works in concert with OGT (O-GlcNAc transferase) which adds O-GlcNAc maintaining a balance important for cellular function.

Pathways

MGEA5 involvement centers largely around glucose homeostasis and the insulin signaling pathway. It modulates the function of proteins like insulin receptor substrate (IRS) and other key regulators in this pathway affecting metabolic regulation and energy homeostasis. It shows a direct relationship with OGT as they reciprocate in action effects highlighting their collaborative role in cellular pathways that affect cellular signaling and survival.

MGEA5 has been implicated in neurodegenerative diseases and diabetes. In Alzheimer's disease altered O-GlcNAc cycling affects tau protein homeostasis which is significant in disease pathogenesis. Likewise in diabetes MGEA5 influences insulin resistance by regulating O-GlcNAc levels on insulin signaling molecules. Dysregulation of these processes may also involve proteins such as beta-amyloid further linking MGEA5 to these pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 1. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins (PubMed : 11148210, PubMed : 11788610, PubMed : 20673219, PubMed : 22365600, PubMed : 24088714, PubMed : 28939839, PubMed : 37962578). Deglycosylates a large and diverse number of proteins, such as CRYAB, ELK1, GSDMD, LMNB1 and TAB1 (PubMed : 28939839, PubMed : 37962578). Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed : 20673219). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed : 24088714).. Isoform 3. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.

See full target information OGA

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 20:20251109 PubMed40771411

2025

Artesunate inhibits hepatocellular carcinoma cell migration and invasion through OGA-mediated -GlcNAcylation of ZEB1.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Li,Yuan Zhang,Wei Lu,Yun Chen,Xiaoling Qian

Nature communications 14:6952 PubMed37907462

2023

Cryo-EM structure of human O-GlcNAcylation enzyme pair OGT-OGA complex.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Lu,Yusong Liu,Maozhou He,Ting Cao,Mengquan Yang,Shutao Qi,Hongtao Yu,Haishan Gao

Journal of molecular cell biology 14: PubMed35285892

2022

Regulation of the urea cycle by CPS1 O-GlcNAcylation in response to dietary restriction and aging.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wu,Jiayu Liu,Kalina Lapenta,Reina Desrouleaux,Min-Dian Li,Xiaoyong Yang

The Journal of biological chemistry 287:11070-81 PubMed22275356

2012

Critical role of O-Linked β-N-acetylglucosamine transferase in prostate cancer invasion, angiogenesis, and metastasis.

Applications

Species

Human

Thomas P Lynch,Christina M Ferrer,S RaElle Jackson,Kristina S Shahriari,Keith Vosseller,Mauricio J Reginato

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MGEA5/OGA antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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