AB183712

Anti-mGluR1a antibody [EPR13540]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal GRM1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

GPRC1A, MGLUR1, GRM1, Metabotropic glutamate receptor 1, mGluR1

2 Images

Lab

Western blot - Anti-mGluR1a antibody [EPR13540] (AB183712)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in PMID : 30375476 and PMID : 11672421.
We recommend not to boil the samples after lysis to get desired WB results.

All lanes:

Western blot - Anti-mGluR1a antibody [EPR13540] (ab183712) at 1/1000 dilution

Lane 1:

Human cerebellum lysate, boiled at 20 µg

Lane 2:

Mouse brain lysate, boiled at 20 µg

Lane 3:

Rat brain tissue lysate, boiled at 20 µg

Lane 4:

Human cerebellum lysate, unboiled at 20 µg

Lane 5:

Mouse brain lysate, unboiled at 20 µg

Lane 6:

Rat brain tissue lysate, unboiled at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 132 kDa

Observed band size: 101 kDa,132 kDa

true

Exposure time: 20s

Supplier Data

Western blot - Anti-mGluR1a antibody [EPR13540] (AB183712)

All lanes:

Western blot - Anti-mGluR1a antibody [EPR13540] (ab183712) at 1/5000 dilution

All lanes:

Human cerebellum lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 132 kDa

Observed band size: 101 kDa,200 kDa

false

Carrier free

Anti-mGluR1a antibody [EPR13540] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13540

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "The molecular weight observed is consistent with what has been described in PMID: 30375476 and PMID: 11672421. We recommend not to boil the samples after lysis to get desired WB results." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "The molecular weight observed is consistent with what has been described in PMID: 30375476 and PMID: 11672421. We recommend not to boil the samples after lysis to get desired WB results." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "The molecular weight observed is consistent with what has been described in PMID: 30375476 and PMID: 11672421. We recommend not to boil the samples after lysis to get desired WB results." } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGluR1a also known as metabotropic glutamate receptor 1a is a G-protein coupled receptor with a mass of approximately 153 kDa. This receptor binds glutamate an excitatory neurotransmitter and initiates intracellular signaling by activating G-proteins. It is expressed in various regions of the brain such as the cerebellum hippocampus and olfactory bulb. The receptor is involved in modulating several synaptic processes by influencing the release of neurotransmitters.

Biological function summary

Glutamate receptor mGluR1a plays a significant role in neuroplasticity and synaptic transmission. It does not act alone but is part of a larger group of metabotropic glutamate receptors. These receptors participate in both excitatory and inhibitory signaling pathways affecting signal regulation and neuron connectivity. mGluR1a contributes to learning and memory processes through its ability to modulate synaptic strength.

Pathways

MGluR1a is an important component in the phosphatidylinositol signaling system and the glutamatergic synapse pathway. Both pathways are related to calcium ion homeostasis and protein trafficking in neurons. Among the related proteins Homer and Shank play a role in the mGluR1a signaling complex and aid in its function within these pathways affecting synaptic structure and plasticity.

MGluR1a is associated with several neuropathological conditions. Alterations in its function or expression can contribute to the development of neurodegenerative diseases like Alzheimer’s disease and psychiatric disorders such as schizophrenia. The receptor interacts with proteins like amyloid precursor protein (APP) and SynGAP which play roles in the etiology and progression of these conditions. Dysregulation of mGluR1a signaling may affect neuronal death and lead to cognitive defects seen in these diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum (PubMed : 24603153, PubMed : 28886343, PubMed : 7476890). May function in the light response in the retina (By similarity). Induces GRID1 and GRID2 cation-channel activation via GNAQ-PLC-PKC pathway in dopaminergic neurons and cerebellar Purkinje cell, respectively (PubMed : 24357660, PubMed : 27276689).

See full target information GRM1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 48: PubMed34528696

2021

circ‑Grm1 promotes pulmonary artery smooth muscle cell proliferation and migration via suppression of GRM1 expression by FUS.

Applications

Unspecified application

Species

Unspecified reactive species

Shijing Sun,Qingyu Kong,Zhifeng Cai,Minmin Wang,Haizhao Zhao,Cuifen Zhao

PLoS pathogens 13:e1006733 PubMed29176838

2017

Inhibition of group-I metabotropic glutamate receptors protects against prion toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Despoina Goniotaki,Asvin K K Lakkaraju,Amulya N Shrivastava,Pamela Bakirci,Silvia Sorce,Assunta Senatore,Rajlakshmi Marpakwar,Simone Hornemann,Fabrizio Gasparini,Antoine Triller,Adriano Aguzzi

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-mGluR1a antibody [EPR13540]

Western blot - Anti-mGluR1a antibody [EPR13540] (AB183712)

Western blot - Anti-mGluR1a antibody [EPR13540] (AB183712)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise