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AB250688

Anti-mGluR1a antibody [EPR13540] - BSA and Azide free

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Rabbit Recombinant Monoclonal GRM1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

GPRC1A, MGLUR1, GRM1, Metabotropic glutamate receptor 1, mGluR1

2 Images
Western blot - Anti-mGluR1a antibody [EPR13540] - BSA and Azide free (AB250688)
  • WB

Supplier Data

Western blot - Anti-mGluR1a antibody [EPR13540] - BSA and Azide free (AB250688)

This data was developed using ab183712, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-mGluR1a antibody [EPR13540] (<a href='/en-us/products/primary-antibodies/mglur1a-antibody-epr13540-ab183712'>ab183712</a>) at 1/5000 dilution

All lanes:

Human cerebellum lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 132 kDa

Observed band size: 101 kDa,200 kDa

false

Western blot - Anti-mGluR1a antibody [EPR13540] - BSA and Azide free (AB250688)
  • WB

Lab

Western blot - Anti-mGluR1a antibody [EPR13540] - BSA and Azide free (AB250688)

This data was developed using ab183712, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in PMID : 30375476 and 11672421.

We recommend not to boil the samples after lysis to get desired WB results.

All lanes:

Western blot - Anti-mGluR1a antibody [EPR13540] (<a href='/en-us/products/primary-antibodies/mglur1a-antibody-epr13540-ab183712'>ab183712</a>) at 1/1000 dilution

Lane 1:

Human cerebellum lysate, boiled at 20 µg

Lane 2:

Mouse brain lysate, boiled at 20 µg

Lane 3:

Rat brain tissue lysate, boiled at 20 µg

Lane 4:

Human cerebellum lysate, unboiled at 20 µg

Lane 5:

Mouse brain lysate, unboiled at 20 µg

Lane 6:

Rat brain tissue lysate, unboiled at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 132 kDa

Observed band size: 101 kDa,132 kDa

true

Exposure time: 20s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13540

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250688 is the carrier-free version of ab183712.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGluR1a also known as metabotropic glutamate receptor 1a is a G-protein coupled receptor with a mass of approximately 153 kDa. This receptor binds glutamate an excitatory neurotransmitter and initiates intracellular signaling by activating G-proteins. It is expressed in various regions of the brain such as the cerebellum hippocampus and olfactory bulb. The receptor is involved in modulating several synaptic processes by influencing the release of neurotransmitters.
Biological function summary

Glutamate receptor mGluR1a plays a significant role in neuroplasticity and synaptic transmission. It does not act alone but is part of a larger group of metabotropic glutamate receptors. These receptors participate in both excitatory and inhibitory signaling pathways affecting signal regulation and neuron connectivity. mGluR1a contributes to learning and memory processes through its ability to modulate synaptic strength.

Pathways

MGluR1a is an important component in the phosphatidylinositol signaling system and the glutamatergic synapse pathway. Both pathways are related to calcium ion homeostasis and protein trafficking in neurons. Among the related proteins Homer and Shank play a role in the mGluR1a signaling complex and aid in its function within these pathways affecting synaptic structure and plasticity.

MGluR1a is associated with several neuropathological conditions. Alterations in its function or expression can contribute to the development of neurodegenerative diseases like Alzheimer’s disease and psychiatric disorders such as schizophrenia. The receptor interacts with proteins like amyloid precursor protein (APP) and SynGAP which play roles in the etiology and progression of these conditions. Dysregulation of mGluR1a signaling may affect neuronal death and lead to cognitive defects seen in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum (PubMed : 24603153, PubMed : 28886343, PubMed : 7476890). May function in the light response in the retina (By similarity). Induces GRID1 and GRID2 cation-channel activation via GNAQ-PLC-PKC pathway in dopaminergic neurons and cerebellar Purkinje cell, respectively (PubMed : 24357660, PubMed : 27276689).
See full target information GRM1
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Product promise

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For full details, please see our Terms & Conditions

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