AB69629

Anti-MGMT antibody

Name: Anti-MGMT antibody (ab69629) | Abcam
Brand: Abcam Limited
SKU: AB69629
Price: 535 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal MGMT antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MGMT.

View Alternative Names

Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, MGMT

1 Images

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Western blot - Anti-MGMT antibody (AB69629)

All lanes:

Western blot - Anti-MGMT antibody (ab69629) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells at 5 µg

Lane 2:

Extracts from Jurkat cells at 5 µg with immunising peptide

Predicted band size: 22 kDa

Observed band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human MGMT. The exact immunogen used to generate this antibody is proprietary information.

P16455

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGMT also known as O-6-methylguanine-DNA methyltransferase is an enzyme with a significant role in DNA repair. MGMT protein has a molecular weight of about 22 kDa. This protein is typically expressed in various tissues including the liver kidney and brain where it functions to protect cells from mutagenic and cytotoxic alterations. MGMT works by transferring alkyl groups from the O6 position of guanine to a cysteine residue within its active site. This mechanism directly reverses DNA damage maintaining genomic stability.

Biological function summary

MGMT prevents mutations by reversing alkylation damage playing a significant role in preserving DNA integrity. It acts independently and does not form part of any larger protein complexes. This enzyme-mediated repair is central to cellular defense against mutagenic threats such as alkylating agents. By curbing DNA damage MGMT can thwart the onset of harmful genetic mutations that might lead to issues like cancer development.

Pathways

MGMT is intimately involved in DNA damage response and repair pathways. Notably it interacts with base excision repair and direct reversal repair pathways. MGMT's function is distinct but complementary to the activities of other DNA repair proteins like XRCC1 and APE1 which are involved in the base excision repair pathway. The presence of MGMT bolsters cellular resilience by ensuring that DNA lesions caused by alkylating agents are addressed promptly and effectively.

MGMT has a profound association with several types of cancer such as glioblastoma and colorectal cancer. Reduced MGMT expression or activity can lead to enhanced susceptibility to the carcinogenic effects of alkylating agents making cancer development more likely. Additionally MGMT inhibitors such as lomeguatrib are used therapeutically to enhance the effectiveness of alkylating chemotherapy. Within this context MGMT interacts with proteins involved in tumor suppression and cell cycle regulation including p53 influencing both cancer progression and treatment outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction : the enzyme is irreversibly inactivated.

See full target information MGMT

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 13:2331-2349 PubMed34017393

2021

Differentiated embryonic chondrocyte-expressed gene 1 promotes temozolomide resistance by modulating the SP1-MGMT axis in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weifeng Lv,Qi Li,Bo Jia,Yalong He,Yi Ru,Qingdong Guo,Xia Li,Wei Lin

CNS neuroscience & therapeutics 27:552-563 PubMed33460245

2021

Regulation of temozolomide resistance in glioma cells via the RIP2/NF-κB/MGMT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hua Hu,Bao-Hua Jiao,Cheng-Ye Wang,Jian-Liang Wu

Journal of cellular physiology 234:6783-6800 PubMed30317578

2018

Resveratrol restores sensitivity of glioma cells to temozolamide through inhibiting the activation of Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Chao Yang,Jun-Yi Wang,Xing-Yao Bu,Bin Yang,Bang-Qing Wang,Sen Hu,Zhao-Yue Yan,Yu-Shuai Gao,Shuang-Yin Han,Ming-Qi Qu

Epigenetics 10:810-8 PubMed26178744

2015

Characterization of CpG sites that escape methylation on the inactive human X-chromosome.

Applications

Unspecified application

Species

Unspecified reactive species

Erika L Moen,Edward Litwin,Stephen Arnovitz,Xu Zhang,Wei Zhang,M Eileen Dolan,Lucy A Godley

Journal of cancer therapy 4:919-931 PubMed23946891

2013

Expression of -Methylguanine-DNA Methyltransferase Examined by Alkyl-Transfer Assays, Methylation-Specific PCR and Western Blots in Tumors and Matched Normal Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Kimiko Ishiguro,Krishnamurthy Shyam,Philip G Penketh,Raymond P Baumann,Alan C Sartorelli,Thomas J Rutherford,Elena S Ratner

Translational oncology 3:50-5 PubMed20165695

2010

Differential Effects of the Proteasome Inhibitor NPI-0052 against Glioma Cells.

Applications

Species

Human

Erina Vlashi,Malcom Mattes,Chann Lagadec,Lorenza Della Donna,Tiffany M Phillips,Polin Nikolay,William H McBride,Frank Pajonk

View all publications

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