Anti-MGST1 antibody [EPR7934]

7 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling MGST1 with Purified ab131059 at 1:1000 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

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  Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)

  Blocking and dilutuing buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-MGST1 antibody [EPR7934] (ab131059) at 1/5000 dilution

  Lane 1: Human fetal liver lysates at 20 µg

  Lane 2: Mouse liver lysates at 20 µg

  Lane 3: Rat liver lysates at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Predicted band size: 18 kDa

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  Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] (ab131059)

  Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MGST1 with purified ab131059 at 1/40 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  

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  Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] (ab131059)

  Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Microsomal Glutathione S-transferase 2 with Purified ab131059 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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  Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)

  All lanes: Western blot - Anti-MGST1 antibody [EPR7934] (ab131059) at 1/1000 dilution

  Lane 1: Human fetal liver lysate at 10 µg

  Lane 2: HepG2 lysate at 10 µg

  Lane 3: HT-1376 lysate at 10 µg

  Secondary

  All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

  Predicted band size: 18 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)

  Immunohistochemical analysis of paraffin embedded Human liver tissue labelling MGST1 with ab131059 antibody at 1/50 dilution.

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  OI-RD Scanning - Anti-MGST1 antibody [EPR7934] (ab131059)

  We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
  Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.