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Rabbit Recombinant Monoclonal MGST1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (AB232469), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (AB232469), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (AB232469), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (AB232469), expandable thumbnail
  • OI-RD Scanning - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (AB232469), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Expected
Tested
Tested
Tested
Mouse
Expected
Predicted
Predicted
Predicted
Rat
Expected
Predicted
Predicted
Predicted

Expected
Expected

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

-

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Product

Target data

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.

Alternative names

Microsomal glutathione S-transferase 1, Microsomal GST-1, Microsomal GST-I, GST12, MGST1, MGST

Recommended products

Rabbit Recombinant Monoclonal MGST1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

Alternative names

Microsomal glutathione S-transferase 1, Microsomal GST-1, Microsomal GST-I, GST12, MGST1, MGST

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR7934

Purification technique

Affinity purification Protein A

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab232469 is the carrier-free version of Anti-MGST1 antibody [EPR7934] ab131059.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MGST1 also known as Microsomal Glutathione S-Transferase 1 is an enzyme involved in the detoxification pathway. This enzyme has a mass of approximately 17 kDa. MGST1 is expressed in various tissues with strong presence in liver and other tissues rich in endoplasmic reticulum. It also participates in protection against oxidative stress due to its role in conjugating reduced glutathione to a wide variety of hydrophobic electrophiles.

Biological function summary

The enzyme acts to catalyze the conjugation of glutathione to electrophilic compounds which helps in neutralizing harmful substances facilitating their excretion. MGST1 forms a homotrimer complex that enhances its activity and stability. This action is vital in maintaining cellular homeostasis and defending against injury from toxic compounds and oxidative stress.

Pathways

MGST1 significantly contributes to the glutathione metabolism and arachidonic acid pathways. In the glutathione metabolism it interacts with glutathione S-transferase enzymes facilitating detoxification processes in the cell. In the arachidonic acid pathway MGST1 cooperates with other enzymes to modulate the synthesis of key molecules involved in inflammation and cell signaling.

Associated diseases and disorders

MGST1 is implicated in the development of liver cancer and cardiovascular diseases. Its overexpression or dysregulation can lead to abnormal detoxification processes and oxidative damage contributing to carcinogenesis. In cardiovascular diseases the enzyme's role in oxidative stress links it to endothelial dysfunction. MGST1's interaction with proteins like CYP450 and GSTs highlights its significance in these disease pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling MGST1 with Purified Anti-MGST1 antibody [EPR7934] ab131059 at 1:1000 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MGST1 antibody [EPR7934] ab131059).

  • Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MGST1with purified Anti-MGST1 antibody [EPR7934] ab131059 at 1/40 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MGST1 antibody [EPR7934] ab131059).

  • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Microsomal Glutathione S-transferase 2 with Purified Anti-MGST1 antibody [EPR7934] ab131059 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MGST1 antibody [EPR7934] ab131059).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labelling MGST1 with Anti-MGST1 antibody [EPR7934] ab131059 antibody at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MGST1 antibody [EPR7934] ab131059).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469), expandable thumbnail

    OI-RD Scanning - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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