AB129175

Anti-MGST1 antibody [EPR7935]

Name: Anti-MGST1 antibody [EPR7935] (ab129175) | Abcam
Brand: Abcam Limited
SKU: AB129175
Price: 275 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal MGST1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications.

View Alternative Names

GST12, MGST, MGST1, Microsomal glutathione S-transferase 1, Microsomal GST-1, Microsomal GST-I

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7935] (AB129175)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7935] (AB129175)

ab129175 at 1/100 staining MGST1 in HepG2 cells by Immunofluorescence.

Lab

Western blot - Anti-MGST1 antibody [EPR7935] (AB129175)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

ab131059 was not effective in the tested cell lines; therefore, ab129175 is recommended as an alternative.

Lanes 1 - 4:

Western blot - Anti-MGST1 antibody [EPR7934] (<a href='/en-us/products/primary-antibodies/mgst1-antibody-epr7934-ab131059'>ab131059</a>) at 1/1000 dilution

Lanes 5 - 8:

Western blot - Anti-MGST1 antibody [EPR7935] (ab129175) at 1/1000 dilution

Lanes 1 and 5:

Mouse liver tissue lysate at 20 µg

Lanes 2 and 6:

Mouse kidney tissue lysate at 20 µg

Lanes 3 and 7:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 4 and 8:

K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa

false

Exposure time: 10s

Unknown

Western blot - Anti-MGST1 antibody [EPR7935] (AB129175)

All lanes:

Western blot - Anti-MGST1 antibody [EPR7935] (ab129175) at 1/1000 dilution

Lane 1:

Fetal liver tissue lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

HT 1376 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 18 kDa

Observed band size: 16 kDa

false

OI-RD Scanning - Anti-MGST1 antibody [EPR7935] (AB129175)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MGST1 antibody [EPR7935] (AB129175)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-MGST1 antibody [EPR7935] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7935

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGST1 also known as Microsomal Glutathione S-Transferase 1 is an enzyme involved in the detoxification pathway. This enzyme has a mass of approximately 17 kDa. MGST1 is expressed in various tissues with strong presence in liver and other tissues rich in endoplasmic reticulum. It also participates in protection against oxidative stress due to its role in conjugating reduced glutathione to a wide variety of hydrophobic electrophiles.

Biological function summary

The enzyme acts to catalyze the conjugation of glutathione to electrophilic compounds which helps in neutralizing harmful substances facilitating their excretion. MGST1 forms a homotrimer complex that enhances its activity and stability. This action is vital in maintaining cellular homeostasis and defending against injury from toxic compounds and oxidative stress.

Pathways

MGST1 significantly contributes to the glutathione metabolism and arachidonic acid pathways. In the glutathione metabolism it interacts with glutathione S-transferase enzymes facilitating detoxification processes in the cell. In the arachidonic acid pathway MGST1 cooperates with other enzymes to modulate the synthesis of key molecules involved in inflammation and cell signaling.

MGST1 is implicated in the development of liver cancer and cardiovascular diseases. Its overexpression or dysregulation can lead to abnormal detoxification processes and oxidative damage contributing to carcinogenesis. In cardiovascular diseases the enzyme's role in oxidative stress links it to endothelial dysfunction. MGST1's interaction with proteins like CYP450 and GSTs highlights its significance in these disease pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

See full target information MGST1

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Pharmacological research 196:106899 PubMed37648102

2023

Microsomal glutathione transferase 1 controls metastasis and therapeutic response in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Zhi-Wei Ye,Paramita Chakraborty,Zhenwu Luo,John Culpepper,Muhammad Aslam,Leilei Zhang,Katarina Johansson,Jesper Z Haeggström,Jianqiang Xu,Magnus Olsson,Danyelle M Townsend,Shikhar Mehrotra,Ralf Morgenstern,Kenneth D Tew

The Journal of biological chemistry 299:104920 PubMed37321450

2023

A role for microsomal glutathione transferase 1 in melanin biosynthesis and melanoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Zhi-Wei Ye,Lars Bräutigam,Paramita Chakraborty,Zhenwu Luo,John Culpepper,Muhammad Aslam,Leilei Zhang,Katarina Johansson,Jesper Z Haeggström,Jianqiang Xu,Magnus Olsson,Danyelle M Townsend,Shikhar Mehrotra,Ralf Morgenstern,Kenneth D Tew

PloS one 11:e0158327 PubMed27438997

2016

Increased THEMIS First Exon Usage in CD4+ T-Cells Is Associated with a Genotype that Is Protective against Multiple Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica L Davies,Sara Thompson,Harpreet Kaur-Sandhu,Stephen Sawcer,Alasdair Coles,Maria Ban,Joanne Jones

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MGST1 antibody [EPR7935]

Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7935] (AB129175)

Western blot - Anti-MGST1 antibody [EPR7935] (AB129175)

Western blot - Anti-MGST1 antibody [EPR7935] (AB129175)

OI-RD Scanning - Anti-MGST1 antibody [EPR7935] (AB129175)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise