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AB251124

Anti-MGST3 antibody [EPR12352] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MGST3 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

GST-3, Glutathione peroxidase MGST3, LTC4 synthase MGST3, MGST3

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)

This data was developed using ab192254, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
(inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)
  • IP

Supplier Data

Immunoprecipitation - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)

This data was developed using ab192254, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab192254 at 1/20 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-MGST3 antibody [EPR12352] (<a href='/en-us/products/primary-antibodies/mgst3-antibody-epr12352-ab192254'>ab192254</a>)

Predicted band size: 17 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)

This data was developed using ab192254, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
(inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)

This data was developed using ab192254, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
(inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)
  • WB

Supplier Data

Western blot - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)

This data was developed using ab192254, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MGST3 antibody [EPR12352] (<a href='/en-us/products/primary-antibodies/mgst3-antibody-epr12352-ab192254'>ab192254</a>) at 1/1000 dilution

All lanes:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 17 kDa

false

Western blot - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)
  • WB

Supplier Data

Western blot - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)

This data was developed using ab192254, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MGST3 antibody [EPR12352] (<a href='/en-us/products/primary-antibodies/mgst3-antibody-epr12352-ab192254'>ab192254</a>) at 1/10000 dilution

Lane 1:

A673 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

false

Western blot - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)
  • WB

Supplier Data

Western blot - Anti-MGST3 antibody [EPR12352] - BSA and Azide free (AB251124)

This data was developed using ab192254, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MGST3 antibody [EPR12352] (<a href='/en-us/products/primary-antibodies/mgst3-antibody-epr12352-ab192254'>ab192254</a>) at 1/1000 dilution

All lanes:

mouse spleen tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12352

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

AB14746

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Primary Antibodies

AB131059

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (AB131059)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab251124 is the carrier-free version of ab192254.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGST3 also known as Microsomal Glutathione S-Transferase 3 is an enzyme involved in the detoxification of electrophilic compounds. It has a molecular mass close to 17 kDa and resides in the endoplasmic reticulum's membrane. MGST3 displays high expression levels in liver and kidney tissues indicating a significant role in these detoxification centers. Together with other microsomal glutathione S-transferases it participates in reducing oxidative stress by conjugating glutathione to a wide range of substrates.
Biological function summary

MGST3 functions as part of a larger enzymatic complex and plays an essential role in xenobiotic metabolism. It protects cells from the toxic effects of various electrophilic compounds aiding the maintenance of cellular health. By facilitating the conversion of lipophilic substances into more water-soluble products MGST3 assists in cellular detoxification processes. This activity underlines the importance of MGST3 in maintaining cellular homeostasis and preventing cellular damage.

Pathways

MGST3 integrates into the glutathione metabolism and arachidonic acid metabolism pathways. In the glutathione pathway MGST3's relationship with glutathione-dependent enzymes supports direct integration into the cell's detoxification processes. Additionally it connects with proteins involved in inflammation and oxidative stress response such as other glutathione S-transferases. By partaking in these pathways MGST3 contributes to the protection against oxidative damage and modulates inflammatory responses.

MGST3 shows relevance to conditions such as drug-induced liver injury and certain types of cancer. Changes in MGST3 expression affect hepatic cellular responses to toxic agents linking it to liver pathologies. In cancer aberrations in MGST3 expression correlate with resistance to chemotherapy making it an area of interest for therapeutic strategies. The enzyme's association with proteins like cytochrome P450 enzymes further implicates its involvement in the metabolic activation of potential carcinogens.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Displays both glutathione S-transferase and glutathione peroxidase activities toward oxyeicosanoids, as part of cellular detoxification as well as synthesis of bioactive metabolites (PubMed : 36370807, PubMed : 9278457). Catalyzes conjugate addition of reduced glutathione to the alpha, beta-unsaturated C=C carbonyl group of eisosanoids such as leukotriene A4 and 15-deoxy-Delta12,14-prostaglandin J2 to form GSH adducts relevant to the inflammatory response (PubMed : 36370807, PubMed : 9278457). Catalyzes glutathione-dependent reduction of eicosanoid peroxides to yield the corresponding eicosanoid hydroxides (PubMed : 9278457).
See full target information MGST3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34769092

2021

11,12 Epoxyeicosatrienoic Acid Rescues Deteriorated Wound Healing in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Sommer,Heike Jakob,Caroline Reiche,Dirk Henrich,Jasmina Sterz,Johannes Frank,Ingo Marzi,Anna Lena Sander
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com