Rabbit Recombinant Monoclonal MGT4A antibody. Suitable for IHC-P, IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Western blot - Anti-MGT4A antibody [EPR10034] (AB151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--western-blot-img66078.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MGT4A antibody [EPR10034] (AB151750)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (AB151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119553.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (AB151750)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (AB151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115127.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (AB151750)")
![Immunocytochemistry/ Immunofluorescence - Anti-MGT4A antibody [EPR10034] (AB151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunocytochemistry-immunofluorescence-img9872.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MGT4A antibody [EPR10034] (AB151750)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (AB151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117829.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (AB151750)")
Publications
Filter by
- PLoS genetics 16:e10091762020Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmily M Schleicher et. al.PubMed 33137164
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Tested | Tested |
| Mouse | Predicted | Expected | Expected | Expected | Predicted |
| Rat | Predicted | Expected | Expected | Expected | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Target data
Function
Glycosyltransferase that catalyze the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains (PubMed:17006639). Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells (By similarity).
Alternative names
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa, GlcNAc-T IVa, GnT-IVa, N-acetylglucosaminyltransferase IVa, MGAT4A
Recommended products
Rabbit Recombinant Monoclonal MGT4A antibody. Suitable for IHC-P, IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR10034
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MGT4A also known as Magnesium Transporter 4A is a protein involved in magnesium ion translocation. Weighing approximately 40 kDa this protein functions as an integral membrane protein primarily facilitating the movement of magnesium ions across cellular membranes. MGT4A can be found expressed in various tissues with significant levels observed in the intestinal epithelium. It plays a critical role in maintaining magnesium homeostasis within cells.
- Biological function summary
The magnesium transporter MGT4A helps regulate intracellular magnesium levels which are important for numerous cellular functions including enzymatic reactions and signal transduction. MGT4A operates as part of a larger magnesium transport complex that is responsible for ensuring the proper uptake and distribution of magnesium ions within cells. This regulation is essential for maintaining the activity of many magnesium-dependent processes within the body.
- Pathways
Magnesium homeostasis is an important function of MGT4A and it participates in cellular metabolism and energy production pathways. MGT4A interacts with proteins involved in the cation transport pathway ensuring balanced magnesium levels which are necessary for ATP production and DNA synthesis. It is also related to the transporter family that includes other cation transport proteins which have roles in similar biological pathways.
- Associated diseases and disorders
Mutations or malfunctions in MGT4A can lead to conditions such as hypomagnesemia and hypertension. These diseases stem from disrupted magnesium levels affecting cardiovascular and epithelial cell functions. In the context of these conditions MGT4A interacts with other transporter proteins like those involved in calcium and sodium ion transport underlining its significance in ion homeostasis and highlighting its potential as a therapeutic target for related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-MGT4A antibody [EPR10034] (ab151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--western-blot-img66078.jpg "Western blot - Anti-MGT4A antibody [EPR10034] (ab151750)")
Western blot - Anti-MGT4A antibody [EPR10034] (ab151750)
All lanes: Western blot - Anti-MGT4A antibody [EPR10034] (ab151750) at 1/1000 dilution
Lane 1: Human fetal heart lysate at 10 µg
Lane 2: Human fetal kidney lysate at 10 µg
Lane 3: HeLa lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 109 kDa, 62 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119553.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)
Immunohistochemical analysis of paraffin-embedded Human choriocarcinoma tissue labeling MGAT4A with ab151750 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115127.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling MGAT4A with ab151750 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-MGT4A antibody [EPR10034] (ab151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunocytochemistry-immunofluorescence-img9872.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MGT4A antibody [EPR10034] (ab151750)")
Immunocytochemistry/ Immunofluorescence - Anti-MGT4A antibody [EPR10034] (ab151750)
Immunofluorescent analysis of HeLa cells labeling MGAT4A with ab151750 at 1/250 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117829.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)
Immunohistochemical analysis of paraffin embedded Human Prostate hyperplasia tissue using ab151750 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130546.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)
Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151750 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98090.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)
Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab151750 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img60475.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)
Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151750 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130546.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98090.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750), expandable thumbnail](https://content.abcam.com/products/images/mgt4a-antibody-epr10034-ab151750--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img60475.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGT4A antibody [EPR10034] (ab151750)")
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