Rabbit Polyclonal mH2A1 antibody. Suitable for IHC-P, ChIP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 35 publications.
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- Nature cell biology 25:1332-13452023MacroH2A restricts inflammatory gene expression in melanoma cancer-associated fibroblasts by coordinating chromatin looping.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Filipescu,Saul Carcamo,Aman Agarwal,Navpreet Tung,Étienne Humblin,Matthew S Goldberg,Nikki S Vyas,Kristin G Beaumont,Deniz Demircioglu,Subhasree Sridhar,Flavia G Ghiraldini,Claudia Capparelli,Andrew E Aplin,Hélène Salmon,Robert Sebra,Alice O Kamphorst,Miriam Merad,Dan Hasson,Emily BernsteinPubMed 37605008
- Communications biology 6:2152023MacroH2A histone variants modulate enhancer activity to repress oncogenic programs and cellular reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWazim Mohammed Ismail,Amelia Mazzone,Flavia G Ghiraldini,Jagneet Kaur,Manvir Bains,Amik Munankarmy,Monique S Bagwell,Stephanie L Safgren,John Moore-Weiss,Marina Buciuc,Lynzie Shimp,Kelsey A Leach,Luis F Duarte,Chandandeep S Nagi,Saul Carcamo,Chi-Yeh Chung,Dan Hasson,Neda Dadgar,Jian Zhong,Jeong-Heon Lee,Fergus J Couch,Alexander Revzin,Tamas Ordog,Emily Bernstein,Alexandre Gaspar-MaiaPubMed 36823213
- Cancer research 82:2887-29032022DLGAP1-AS2-Mediated Phosphatidic Acid Synthesis Activates YAP Signaling and Confers Chemoresistance in Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYabing Nan,Qingyu Luo,Xiaowei Wu,Shi Liu,Pengfei Zhao,Wan Chang,Aiping Zhou,Zhihua LiuPubMed 35731019
- eLife 11:2022Distinct and diverse chromatin proteomes of ageing mouse organs reveal protein signatures that correlate with physiological functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiorgio Oliviero,Sergey Kovalchuk,Adelina Rogowska-Wrzesinska,Veit Schwämmle,Ole N JensenPubMed 35259090
- Science advances 8:eabe43752022A balanced Oct4 interactome is crucial for maintaining pluripotency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Han,Guangming Wu,Rui Chen,Hannes C A Drexler,Caitlin M MacCarthy,Kee-Pyo Kim,Kenjiro Adachi,Daniela Gerovska,Lampros Mavrommatis,Ivan Bedzhov,Marcos J Araúzo-Bravo,Hans R SchölerPubMed 35171666
- Cell reports 36:1096262021Mutant U2AF1-induced alternative splicing of H2afy (macroH2A1) regulates B-lymphopoiesis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanghyun P Kim,Sridhar N Srivatsan,Monique Chavez,Cara L Shirai,Brian S White,Tanzir Ahmed,Michael O Alberti,Jin Shao,Ryan Nunley,Lynn S White,Jeff Bednarski,John R Pehrson,Matthew J WalterPubMed 34469727
- Nature communications 12:35202021The epigenetic regulator LSH maintains fork protection and genomic stability via MacroH2A deposition and RAD51 filament formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoping Xu,Kai Ni,Yafeng He,Jianke Ren,Chongkui Sun,Yie Liu,Mirit I Aladjem,Sandra Burkett,Richard Finney,Xia Ding,Shyam K Sharan,Kathrin MueggePubMed 34112784
- EMBO reports 22:e521502021MacroH2A1.2 deficiency leads to neural stem cell differentiation defects and autism-like behaviors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Ma,Libo Su,Wenlong Xia,Wenwen Wang,Guohe Tan,Jianwei JiaoPubMed 34046991
- The Journal of cell biology 220:2021Poly(ADP-ribose) binding and macroH2A mediate recruitment and functions of KDM5A at DNA lesions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRamhari Kumbhar,Anthony Sanchez,Jullian Perren,Fade Gong,David Corujo,Frank Medina,Sravan K Devanathan,Blerta Xhemalce,Andreas Matouschek,Marcus Buschbeck,Bethany A Buck-Koehntop,Kyle M MillerPubMed 34003252
- Nature communications 11:60532020Trans- and cis-acting effects of Firre on epigenetic features of the inactive X chromosome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHe Fang et. al.PubMed 33247132
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | ChIP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Predicted | Expected | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
ChIP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
Associated Products
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Target data
Function
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription (PubMed:12718888, PubMed:15621527, PubMed:16428466). Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template (PubMed:15897469). Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability (PubMed:15897469). DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation (PubMed:15897469). Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes (PubMed:12718888, PubMed:16428466). Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin (PubMed:16107708, PubMed:16428466). Isoform 1. Isoform that specifically binds poly-ADP-ribose and O-acetyl-ADP-ribose and plays a key role in NAD(+) metabolism (PubMed:15902274). Able to bind to the ends of poly-ADP-ribose chains created by PARP1 and cap them (By similarity). This prevents PARP1 from further addition of ADP-ribose and thus limits the consumption of nuclear NAD(+), allowing the cell to maintain proper NAD(+) levels in both the nucleus and the mitochondria to promote proper mitochondrial respiration (By similarity). Increases the expression of genes involved in redox metabolism, including SOD3 (PubMed:23022728). Isoform 2. In contrast to isoform 1, does not bind poly-ADP-ribose (PubMed:15902274). Represses SOD3 gene expression (PubMed:23022728).
Alternative names
H2AFY, MACROH2A1, Core histone macro-H2A.1, Histone macroH2A1, mH2A1, Histone H2A.y, Medulloblastoma antigen MU-MB-50.205, H2A/y
Recommended products
Rabbit Polyclonal mH2A1 antibody. Suitable for IHC-P, ChIP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 35 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
ab37264 recognises the three known isoforms of mH2A1 including mH2A1.2 (longest isoform) and the mH2A1.1 (shortest isoform).
We have had varying reports about the efficiency with which this antibody recognises mH2A1 in mouse cells and tissues. Please contact our Scientific Support team if you have any queries about this.- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MH2A1 also known as macroH2A1 is a histone variant with a distinguished structure characterized by an extended C-terminal macro domain. It has a molecular mass of approximately 42-45 kDa. This histone variant is widely expressed across various tissues showing particularly high levels in differentiated cells. mH2A1 incorporates into chromatin impacting nucleosome stability and chromatin dynamics. Researchers have also identified an isoform known as H2AFY which further highlights the structural complexity and versatile roles of mH2A1 in cellular regulation.
- Biological function summary
MH2A1 plays significant roles in chromatin remodeling and gene expression regulation. It is part of the nucleosome replacing canonical histone H2A and influencing the accessibility of chromatin to transcriptional machinery. Through this function mH2A1 can repress or activate gene expression depending on cellular context and interacting partners. Its incorporation into chromatin is associated with the silencing of certain genomic regions such as inactive X chromosome in females with potential implications for epigenetic modifications and cellular differentiation.
- Pathways
MH2A1 is notably involved in pathways regulating chromatin organization and cell differentiation. It plays a role in the Polycomb Repressive Complex 2 (PRC2) pathway affecting histone methylation and gene silencing. Furthermore mH2A1 interacts with proteins like EZH2 in PRC2 which carry out methylation at histone H3 on lysine 27. These interactions highlight mH2A1's involvement in maintaining repressive chromatin states and controlling gene expression patterns important for normal cell function.
- Associated diseases and disorders
MH2A1 is connected with cancer and metabolic diseases. Its altered expression has been observed in various cancers where it might impact tumor progression by modulating genes linked to proliferation and differentiation. mH2A1 influences pathways shared with proteins like BRAC1 that are involved in DNA repair processes. Additionally changes in mH2A1 expression are linked to metabolic disorders especially those affecting lipid metabolism and insulin responses suggesting its potential role in regulating metabolic pathways and influencing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-mH2A1 antibody (ab37264)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: MH2A1 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: HepG2 whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab37264 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab37264 was shown to specifically recognize MH2A1 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when MH2A1 knockout cells were examined. Wild-type and MH2A1 knockout samples were subjected to SDS-PAGE. ab37264 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-mH2A1 antibody (ab37264)
Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody (ab37264)
IHC image of mH2A1 staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37264, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - Anti-mH2A1 antibody (ab37264)
All lanes: Western blot - Anti-mH2A1 antibody (ab37264) at 1 µg
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 100 kDa, 40 kDa
Exposure time: 16min
Immunocytochemistry/ Immunofluorescence - Anti-mH2A1 antibody (ab37264)
ab37264 staining mH2A1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab37264 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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