Skip to main content

Rabbit Recombinant Monoclonal mH2A1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.


Images

Western blot - Anti-mH2A1 antibody [EPR9358] (AB137117), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (AB137117), expandable thumbnail
  • Western blot - Anti-mH2A1 antibody [EPR9358] (AB137117), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (AB137117), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (AB137117), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Mouse
Predicted
Not recommended
Not recommended
Predicted
Not recommended
Rat
Predicted
Not recommended
Not recommended
Predicted
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription (PubMed:12718888, PubMed:15621527, PubMed:16428466). Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template (PubMed:15897469). Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability (PubMed:15897469). DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation (PubMed:15897469). Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes (PubMed:12718888, PubMed:16428466). Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin (PubMed:16107708, PubMed:16428466). Isoform 1. Isoform that specifically binds poly-ADP-ribose and O-acetyl-ADP-ribose and plays a key role in NAD(+) metabolism (PubMed:15902274). Able to bind to the ends of poly-ADP-ribose chains created by PARP1 and cap them (By similarity). This prevents PARP1 from further addition of ADP-ribose and thus limits the consumption of nuclear NAD(+), allowing the cell to maintain proper NAD(+) levels in both the nucleus and the mitochondria to promote proper mitochondrial respiration (By similarity). Increases the expression of genes involved in redox metabolism, including SOD3 (PubMed:23022728). Isoform 2. In contrast to isoform 1, does not bind poly-ADP-ribose (PubMed:15902274). Represses SOD3 gene expression (PubMed:23022728).

Alternative names

H2AFY, MACROH2A1, Core histone macro-H2A.1, Histone macroH2A1, mH2A1, Histone H2A.y, Medulloblastoma antigen MU-MB-50.205, H2A/y

Recommended products

Rabbit Recombinant Monoclonal mH2A1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9358
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MH2A1 also known as macroH2A1 is a histone variant with a distinguished structure characterized by an extended C-terminal macro domain. It has a molecular mass of approximately 42-45 kDa. This histone variant is widely expressed across various tissues showing particularly high levels in differentiated cells. mH2A1 incorporates into chromatin impacting nucleosome stability and chromatin dynamics. Researchers have also identified an isoform known as H2AFY which further highlights the structural complexity and versatile roles of mH2A1 in cellular regulation.

Biological function summary

MH2A1 plays significant roles in chromatin remodeling and gene expression regulation. It is part of the nucleosome replacing canonical histone H2A and influencing the accessibility of chromatin to transcriptional machinery. Through this function mH2A1 can repress or activate gene expression depending on cellular context and interacting partners. Its incorporation into chromatin is associated with the silencing of certain genomic regions such as inactive X chromosome in females with potential implications for epigenetic modifications and cellular differentiation.

Pathways

MH2A1 is notably involved in pathways regulating chromatin organization and cell differentiation. It plays a role in the Polycomb Repressive Complex 2 (PRC2) pathway affecting histone methylation and gene silencing. Furthermore mH2A1 interacts with proteins like EZH2 in PRC2 which carry out methylation at histone H3 on lysine 27. These interactions highlight mH2A1's involvement in maintaining repressive chromatin states and controlling gene expression patterns important for normal cell function.

Associated diseases and disorders

MH2A1 is connected with cancer and metabolic diseases. Its altered expression has been observed in various cancers where it might impact tumor progression by modulating genes linked to proliferation and differentiation. mH2A1 influences pathways shared with proteins like BRAC1 that are involved in DNA repair processes. Additionally changes in mH2A1 expression are linked to metabolic disorders especially those affecting lipid metabolism and insulin responses suggesting its potential role in regulating metabolic pathways and influencing disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-mH2A1 antibody [EPR9358] (ab137117), expandable thumbnail

    Western blot - Anti-mH2A1 antibody [EPR9358] (ab137117)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: mH2A1 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: Hepg2 whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab137117 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab137117 was shown to recognize mH2A1 when mH2A1 knockout samples were used, along with additional cross-reactive bands. Wild-type and mH2A1 knockout samples were subjected to SDS-PAGE. ab137117 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-mH2A1 antibody [EPR9358] (ab137117)

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (ab137117), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (ab137117)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver from wild-type and mH2A1/2 knock out tissue sections labeling mH2A1 with ab137117 at 1/400 dilution. Sections were fixed in formaldehyde; heat mediated antigen retivial was performed using a citrate buffer pH 6. An undiluted polyclonal horse anti-rabbit IgG (HRP-conjugated) was used as the secondary antibody.

  • Western blot - Anti-mH2A1 antibody [EPR9358] (ab137117), expandable thumbnail

    Western blot - Anti-mH2A1 antibody [EPR9358] (ab137117)

    All lanes: Western blot - Anti-mH2A1 antibody [EPR9358] (ab137117) at 1/1000 dilution

    Lane 1: Fetal kidney tissue lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: MCF7 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (ab137117), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (ab137117)

    Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labelling mH2A1 with ab137117 antibody at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (ab137117), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9358] (ab137117)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling mH2A1 with ab137117 antibody at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com