Anti-mH2A1 antibody [EPR9359(2)] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9359(2)] - BSA and Azide free (AB232602)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling mH2A1 with ab183041 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183041).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-mH2A1 antibody [EPR9359(2)] - BSA and Azide free (AB232602)

Immunofluorescent analysis of acetone-fixed HeLa cells labeling mH2A1 with ab183041 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). Counter stained with Dapi (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183041).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-mH2A1 antibody [EPR9359(2)] - BSA and Azide free (AB232602)

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling mH2A1 with ab183041 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). Counter stained with Dapi (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183041).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9359(2)] - BSA and Azide free (AB232602)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling mH2A1 with ab183041 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183041).

• IHC-P

AbReview57326****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A1 antibody [EPR9359(2)] - BSA and Azide free (AB232602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver from wild-type and mH2A1/2 knock out tissue sections labeling mH2A1 with ab183041 at 1/400 dilution. Sections were fixed in formaldehyde; heat mediated antigen retivial was performed using a citrate buffer pH 6. An undiluted polyclonal horse anti-rabbit IgG (HRP-conjugated) was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183041).

This image is courtesy of an anonymous abreview.

• WB

Lab

Western blot - Anti-mH2A1 antibody [EPR9359(2)] - BSA and Azide free (AB232602)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : mH2A1 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : Hepg2 whole cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab183041 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab183041 was shown to specifically react with mH2A1 when mH2A1 knockout samples were used. Wild-type and mH2A1 knockout samples were subjected to SDS-PAGE. ab183041 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183041).

All lanes:

Western blot - Anti-mH2A1 antibody [EPR9359(2)] (<a href='/en-us/products/primary-antibodies/mh2a1-antibody-epr93592-ab183041'>ab183041</a>)

Predicted band size: 40 kDa

false

• WB

Lab

Western blot - Anti-mH2A1 antibody [EPR9359(2)] - BSA and Azide free (AB232602)

This data was developed using the same antibody clone in a different buffer formulation (ab183041).

Lanes 1- 2 : Merged signal (red and green). Green - ab183041 observed at 40 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

ab183041 was shown to react with mH2A1 in wild-type HEK-293T cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab266241 (CRISPR/Cas9 edited cell lysate ab257463) lane below 40kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and H2AFY CRISPR/Cas9 edited HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab183041 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-mH2A1 antibody [EPR9359(2)] (<a href='/en-us/products/primary-antibodies/mh2a1-antibody-epr93592-ab183041'>ab183041</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

H2AFY CRISPR/Cas9 edited HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human H2AFY (mH2A1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-h2afy-mh2a1-knockout-hek-293t-cell-line-ab266241'>ab266241</a>)

Predicted band size: 40 kDa

Observed band size: 40 kDa

false