AB102126

Anti-mH2A2 antibody

Name: Anti-mH2A2 antibody (ab102126) | Abcam
Brand: Abcam Limited
SKU: AB102126
Rating: 2 (4 reviews)

(4 Reviews)

(4 Publications)

Rabbit Polyclonal mH2A2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MACROH2A2 aa 150-350.

View Alternative Names

H2AFY2, MACROH2A2, Core histone macro-H2A.2, Histone macroH2A2, mH2A2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB102126)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB102126)

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using ab102126 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB102126)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labelling Macro H2A.2 protein at nucleus with ab102126 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-mH2A2 antibody (AB102126)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-mH2A2 antibody (ab102126) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

human H2AFY2-transfected 293T cells lysates at 30 µg

Predicted band size: 40 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MACROH2A2 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

Q9P0M6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MH2A2 also known as macroH2A2 is a variant of the histone H2A family with a distinct structural feature that includes a large macro domain. This protein weighs approximately 42 kDa and plays roles in chromatin organization and transcription regulation. The expression of mH2A2 occurs in various tissues with notable presence in the liver and testis. It acts by incorporating itself into nucleosomes affecting chromatin accessibility and influencing gene expression.

Biological function summary

MH2A2 influences processes such as gene silencing DNA repair and cellular differentiation. It is part of the nucleosome complex where its presence can result in a more compact chromatin structure leading to decreased gene activity. mH2A2 contributes to X-chromosome inactivation in females by associating with the inactive X chromosome. Moreover it has a role in the cellular response to DNA damage where it helps in maintaining genome stability.

Pathways

MH2A2 is involved in the chromatin remodeling and transcription regulation pathways. It interacts with proteins like histone deacetylases (HDACs) and polycomb repressive complexes impacting epigenetic gene regulation. Through these pathways mH2A2 participates in controlling developmental processes and cellular differentiation linking its function to the A/B compartments of chromatin. This regulation ultimately influences cell-specific gene expression patterns and cellular identity.

MH2A2 has been linked to cancer and metabolic disorders. Studies have shown altered expression of mH2A2 in certain cancers where it associates with tumor suppressor proteins like p53 impacting cellular growth and apoptosis. In metabolic disorders changes in mH2A2 levels affect liver metabolism potentially contributing to conditions such as non-alcoholic fatty liver disease (NAFLD). Understanding the role of mH2A2 in these diseases could provide insights into developing novel therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.

See full target information MACROH2A2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PloS one 18:e0288005 PubMed37432970

2023

Combinatorial targeting of a specific EMT/MET network by macroH2A variants safeguards mesenchymal identity.

Applications

Unspecified application

Species

Unspecified reactive species

Dimitrios Valakos,Eleftheria Klagkou,Antonis Kokkalis,Alexandros Polyzos,Fotis L Kyrilis,Aggelos Banos,Giannis Vatsellas,Maria Pliatska,Ethan Ford,Dimitrios J Stravopodis,Dimitris Thanos

Nucleic acids research 49:8024-8036 PubMed34223906

2021

LSH catalyzes ATP-driven exchange of histone variants macroH2A1 and macroH2A2.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ni,Kathrin Muegge

Nature communications 12:3520 PubMed34112784

2021

The epigenetic regulator LSH maintains fork protection and genomic stability via MacroH2A deposition and RAD51 filament formation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Xu,Kai Ni,Yafeng He,Jianke Ren,Chongkui Sun,Yie Liu,Mirit I Aladjem,Sandra Burkett,Richard Finney,Xia Ding,Shyam K Sharan,Kathrin Muegge

Nucleic acids research 44:5148-60 PubMed26939888

2016

Chromosome intermingling-the physical basis of chromosome organization in differentiated cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shovamayee Maharana,K Venkatesan Iyer,Nikhil Jain,Mallika Nagarajan,Yejun Wang,G V Shivashankar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-mH2A2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB102126)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB102126)

Western blot - Anti-mH2A2 antibody (AB102126)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise