Rabbit Polyclonal mH2A2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MACROH2A2 aa 150-350.
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Publications
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- PloS one 18:e02880052023Combinatorial targeting of a specific EMT/MET network by macroH2A variants safeguards mesenchymal identity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDimitrios Valakos,Eleftheria Klagkou,Antonis Kokkalis,Alexandros Polyzos,Fotis L Kyrilis,Aggelos Banos,Giannis Vatsellas,Maria Pliatska,Ethan Ford,Dimitrios J Stravopodis,Dimitris ThanosPubMed 37432970
- Nucleic acids research 49:8024-80362021LSH catalyzes ATP-driven exchange of histone variants macroH2A1 and macroH2A2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Ni,Kathrin MueggePubMed 34223906
- Nature communications 12:35202021The epigenetic regulator LSH maintains fork protection and genomic stability via MacroH2A deposition and RAD51 filament formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoping Xu,Kai Ni,Yafeng He,Jianke Ren,Chongkui Sun,Yie Liu,Mirit I Aladjem,Sandra Burkett,Richard Finney,Xia Ding,Shyam K Sharan,Kathrin MueggePubMed 34112784
- Nucleic acids research 44:5148-602016Chromosome intermingling-the physical basis of chromosome organization in differentiated cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShovamayee Maharana et. al.PubMed 26939888
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MACROH2A2 aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P0M6
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0) |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
Alternative names
H2AFY2, MACROH2A2, Core histone macro-H2A.2, Histone macroH2A2, mH2A2
Recommended products
Rabbit Polyclonal mH2A2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MACROH2A2 aa 150-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MACROH2A2 aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P0M6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MH2A2 also known as macroH2A2 is a variant of the histone H2A family with a distinct structural feature that includes a large macro domain. This protein weighs approximately 42 kDa and plays roles in chromatin organization and transcription regulation. The expression of mH2A2 occurs in various tissues with notable presence in the liver and testis. It acts by incorporating itself into nucleosomes affecting chromatin accessibility and influencing gene expression.
- Biological function summary
MH2A2 influences processes such as gene silencing DNA repair and cellular differentiation. It is part of the nucleosome complex where its presence can result in a more compact chromatin structure leading to decreased gene activity. mH2A2 contributes to X-chromosome inactivation in females by associating with the inactive X chromosome. Moreover it has a role in the cellular response to DNA damage where it helps in maintaining genome stability.
- Pathways
MH2A2 is involved in the chromatin remodeling and transcription regulation pathways. It interacts with proteins like histone deacetylases (HDACs) and polycomb repressive complexes impacting epigenetic gene regulation. Through these pathways mH2A2 participates in controlling developmental processes and cellular differentiation linking its function to the A/B compartments of chromatin. This regulation ultimately influences cell-specific gene expression patterns and cellular identity.
- Associated diseases and disorders
MH2A2 has been linked to cancer and metabolic disorders. Studies have shown altered expression of mH2A2 in certain cancers where it associates with tumor suppressor proteins like p53 impacting cellular growth and apoptosis. In metabolic disorders changes in mH2A2 levels affect liver metabolism potentially contributing to conditions such as non-alcoholic fatty liver disease (NAFLD). Understanding the role of mH2A2 in these diseases could provide insights into developing novel therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-mH2A2 antibody (ab102126)
10% SDS PAGE
All lanes: Western blot - Anti-mH2A2 antibody (ab102126) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 23 kDa, 37 kDa, 38 kDa, 40 kDa, 97 kDa
Observed band size: 97 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (ab102126)
Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using ab102126 at 1/100 dilution.
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