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AB246995

Anti-mH2A2 antibody

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Rabbit Polyclonal mH2A2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MACROH2A2 aa 150-300.

View Alternative Names

H2AFY2, MACROH2A2, Core histone macro-H2A.2, Histone macroH2A2, mH2A2

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB246995)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB246995)

Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for mH2A2 when incubated with ab246995 at a 1/500 dilution (negative control).

Immunocytochemistry/ Immunofluorescence - Anti-mH2A2 antibody (AB246995)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-mH2A2 antibody (AB246995)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for mH2A2 (green) using ab246995 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB246995)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB246995)

Formalin-fixed, paraffin-embedded human endometrium tissue stained for mH2A2 with ab246995 at a 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB246995)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB246995)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for mH2A2 with ab246995 at a 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB246995)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB246995)

Formalin-fixed, paraffin-embedded human testis tissue stained for mH2A2 with ab246995 at a 1/500 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MACROH2A2 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q9P0M6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MH2A2 also known as macroH2A2 is a variant of the histone H2A family with a distinct structural feature that includes a large macro domain. This protein weighs approximately 42 kDa and plays roles in chromatin organization and transcription regulation. The expression of mH2A2 occurs in various tissues with notable presence in the liver and testis. It acts by incorporating itself into nucleosomes affecting chromatin accessibility and influencing gene expression.
Biological function summary

MH2A2 influences processes such as gene silencing DNA repair and cellular differentiation. It is part of the nucleosome complex where its presence can result in a more compact chromatin structure leading to decreased gene activity. mH2A2 contributes to X-chromosome inactivation in females by associating with the inactive X chromosome. Moreover it has a role in the cellular response to DNA damage where it helps in maintaining genome stability.

Pathways

MH2A2 is involved in the chromatin remodeling and transcription regulation pathways. It interacts with proteins like histone deacetylases (HDACs) and polycomb repressive complexes impacting epigenetic gene regulation. Through these pathways mH2A2 participates in controlling developmental processes and cellular differentiation linking its function to the A/B compartments of chromatin. This regulation ultimately influences cell-specific gene expression patterns and cellular identity.

MH2A2 has been linked to cancer and metabolic disorders. Studies have shown altered expression of mH2A2 in certain cancers where it associates with tumor suppressor proteins like p53 impacting cellular growth and apoptosis. In metabolic disorders changes in mH2A2 levels affect liver metabolism potentially contributing to conditions such as non-alcoholic fatty liver disease (NAFLD). Understanding the role of mH2A2 in these diseases could provide insights into developing novel therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
See full target information MACROH2A2
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