AB326950

Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal HA11 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Transfected cell lysate - Mouse, Mouse, Transfected cell line - Mouse samples.

View Alternative Names

H-2D(B), H2-D1

14 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) : HEK-293T (human embryonic kidney epithelial cell) cells transfected with a mouse D-B expression vector containing a his tag. (B) : HEK-293T cells transfected with a mouse D-D expression vector containing a his tag. (C) : HEK-293T cells transfected with a mouse α chain expression vector containing a his tag. (D) : HEK-293T cells transfected with a mouse D-P expression vector containing a his tag. (E) : HEK-293T cells transfected with a mouse D-K expression vector containing a his tag. (F) : HEK-293T cells transfected with empty vector containing a his tag. tissue labeling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/5000 (0.1 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T cells transfected with a mouse D-B expression vector containing a his tag, (B) HEK-293T cells transfected with a mouse D-D expression vector containing a his tag, (C) HEK-293T cells transfected with a mouse α chain expression vector containing a his tag, (D) HEK-293T cells transfected with a mouse D-P expression vector containing a his tag, and (E) HEK-293T cells transfected with a mouse D-K expression vector containing a his tag, no staining on (F) HEK-293T cells transfected with empty vector containing a his tag.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) : HEK-293T cells transfected with a mouse H2-L expression vector containing a his tag. (B) : HEK-293T cells transfected with a mouse H2-Q10 expression vector containing a his tag. (C) : HEK-293T cells transfected with a mouse H2-Q8 expression vector containing a his tag. (D) : HEK-293T cells transfected with a mouse H2-Q7 expression vector containing a his tag. (E) : HEK-293T cells transfected with a mouse PH-2D expression vector containing a his tag. tissue labeling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/5000 (0.1 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T cells transfected with a mouse H2-L expression vector containing a his tag, (B) HEK-293T cells transfected with a mouse H2-Q10 expression vector containing a his tag, (C) HEK-293T cells transfected with a mouse H2-Q8 expression vector containing a his tag, (D) HEK-293T cells transfected with a mouse H2-Q7 expression vector containing a his tag, and (E) HEK-293T cells transfected with a mouse PH-2D expression vector containing a his tag.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse large B cell lymphoma tissue labeling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/2000 (0.251 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse large B cell lymphoma.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/2000 (0.251 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : weak staining on vascular endothelium of mouse skeletal muscle.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/2000 (0.251 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse spleen.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) : HEK-293T cells transfected with a mouse K-B expression vector containing a his tag. (B) : HEK-293T cells transfected with a mouse K-D expression vector containing a his tag. (C) : HEK-293T cells transfected with a mouse K-K expression vector containing a his tag. (D) : HEK-293T cells transfected with a mouse K-Q expression vector containing a his tag. (E) : HEK-293T cells transfected with a mouse K-W28 expression vector containing a his tag. tissue labeling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/5000 (0.1 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T cells transfected with a mouse K-B expression vector containing a his tag, (B) HEK-293T cells transfected with a mouse K-D expression vector containing a his tag, (C) HEK-293T cells transfected with a mouse K-K expression vector containing a his tag, (D) HEK-293T cells transfected with a mouse K-Q expression vector containing a his tag, and (E) HEK-293T cells transfected with a mouse K-W28 expression vector containing a his tag.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/500 (1.002 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in EL4 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Low expression : Raw264.7. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an empty expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-B alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-D alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-P alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-K alpha chain expression vector containing a myc-His-tag® cells labelling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/1000 (0.501 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-B alpha chain, D-D alpha chain, alpha chain, D-P alpha chain and D-P alpha chain expression vectors containing a myc-His-tag® (shown in green), showing no staining in 293T cells transfected with an empty expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Panel A : 293T cells transfected with an empty expression vector containing a myc-His-tag®. Panel B : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-B alpha chain expression vector containing a myc-His-tag®. Panel C : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-D alpha chain expression vector containing a myc-His-tag®. Panel D : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, alpha chain expression vector containing a myc-His-tag®. Panel E : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-P alpha chain expression vector containing a myc-His-tag®. Panel F : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-K alpha chain expression vector containing a myc-His-tag®.

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-B alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-D alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-K alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-Q alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-W28 alpha chain expression vector containing a myc-His-tag® cells labelling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/1000 (0.501 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-P alpha chain, D-K alpha chain, K-B alpha chain, K-D alpha chain, K-K alpha chain, K-Q alpha chain and K-W28 alpha chain expression vectors containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Panel G : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-B alpha chain expression vector containing a myc-His-tag®. Panel H : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-D alpha chainexpression vector containing a myc-His-tag®. Panel I : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-K alpha chain expression vector containing a myc-His-tag®. Panel J : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-Q alpha chain expression vector containing a myc-His-tag® Panel K : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-W28 alpha chain expression vector containing a myc-His-tag®

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells transfected with a mouse H-2 class I histocompatibility antigen, L-D alpha chain (H2-L) expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, Q10 alpha chain (H2-Q10)expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, Q8 alpha chain (H2-Q8) expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, Q8 alpha chain (H2-Q7) expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, alpha chain, Clone PH-2D-2 expression vector containing a myc-His-tag® cells labelling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/1000 (0.501 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a mouse H-2 class I histocompatibility antigen, L-D alpha chain (H2-L), Q10 alpha chain (H2-Q10), Q8 alpha chain (H2-Q8), Q8 alpha chain (H2-Q7) and alpha chain, Clone PH-2D-2 expression vectors containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Panel L : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, L-D alpha chain (H2-L) expression vector containing a myc-His-tag® Panel M : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, Q7 alpha chain (H2-Q7) expression vector containing a myc-His-tag® Panel N : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, Q8 alpha chain (H2-Q8) expression vector containing a myc-His-tag® Panel O : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, Q7 alpha chain (H2-Q7) expression vector containing a myc-His-tag® Panel P : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, alpha chain, Clone PH-2D-2 expression vector containing a myc-His-tag®

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Lab

Western blot - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Raw 264.7(PMID : 35965334)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa);Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(37-50KDa).

All lanes:

Western blot - Anti-MHC Class 1 antibody [MJF-D30007-535] (<a href='/en-us/products/primary-antibodies/mhc-class-1-antibody-mjf-d30007-535-ab326943'>ab326943</a>) at 1/1000 dilution

Lane 1:

Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Lane 3:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-50 kDa,36 kDa

false

Exposure time: 10s

Lab

Western blot - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa);Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(37-50KDa).

Exposure time : Lanes1-6 : 37 seconds

All lanes:

Western blot - Anti-MHC Class 1 antibody [MJF-D30007-535] (<a href='/en-us/products/primary-antibodies/mhc-class-1-antibody-mjf-d30007-535-ab326943'>ab326943</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse D-B expression vector containing a his tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse D-D expression vector containing a his tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse α chain expression vector containing a his tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse D-P expression vector containing a his tag, whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a mouse D-K expression vector containing a his tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-50 kDa,36 kDa

false

Lab

Western blot - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa);Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(37-50KDa).

Exposure time : Lanes1-5 : 37 seconds

All lanes:

Western blot - Anti-MHC Class 1 antibody [MJF-D30007-535] (<a href='/en-us/products/primary-antibodies/mhc-class-1-antibody-mjf-d30007-535-ab326943'>ab326943</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a mouse K-B expression vector containing a his tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse K-D expression vector containing a his tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse K-K expression vector containing a his tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse K-Q expression vector containing a his tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse K-W28 expression vector containing a his tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-50 kDa,36 kDa

false

Lab

Western blot - Anti-MHC Class 1 antibody [MJF-D30007-535] - BSA and Azide free (AB326950)

This data was developed using ab326943, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa);Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(37-50KDa).

Exposure time : Lanes1-4 : 37 seconds, lane 5 : 8 seconds

All lanes:

Western blot - Anti-MHC Class 1 antibody [MJF-D30007-535] (<a href='/en-us/products/primary-antibodies/mhc-class-1-antibody-mjf-d30007-535-ab326943'>ab326943</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a mouse H2-Q10 expression vector containing a his tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse H2-Q8 expression vector containing a his tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse H2Q7 expression vector containing a his tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse H2-L expression vector containing a his tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse PH-2D expression vector containing a his tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-50 kDa,36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-D30007-535

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody cross-reacts with H2-D: (D-B, D-D, H-2 class I histocompatibility antigen, alpha chain, D-P, D-K), H2-K: (K-B, K-D, K-K, K-Q, K-W28 alpha chain)], H2-L, H2-Q10, H2-Q8, H2-Q7, PH-2D-2. The immunogen used for this clone is targeting H2 D-B/H2-D1.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

ab326950 is the carrier-free version of ab326943

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the presentation of foreign antigens to the immune system.

See full target information H2-D1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com