AB25244

Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]

Name: Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (ab25244) | Abcam
Brand: Abcam Limited
SKU: AB25244
Price: 220 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal HA11 antibody. Suitable for Flow Cyt, FuncS, IHC-Fr and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing MHC Class 1 H2 Db/H2-D1 protein.

View Alternative Names

H-2D(B), H2-D1

578 PE

PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]
519 FITC

FITC Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

27-11-13

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

FuncS, IHC-Fr, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Recognizes an epitope on the a chain of H2Db.

Specificity

ab25244 recognizes the a3 domain of H2Db class I MHC antigen. The antibody cross-reacts with the a3 domain of H2Ld, H2Dq and H2Lq, but not H2Kd or H2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "Complement-dependent cytoxicity assay.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

ab25244 can be used for complement-dependent cytoxicity assays.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 8.2 Constituents: 100% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MHC Class I H2-D1 also known as H2-Db is an important protein within the major histocompatibility complex. This protein carries a molecular mass of approximately 45 kDa. H2-Db is expressed on the surface of almost all nucleated cells. As a protein it plays an important role in immune response by presenting peptide antigens to cytotoxic T cells. Other similar proteins like H2-Kb and H2-Kd are also involved in antigen presentation though each has distinct peptide binding preferences.

Biological function summary

The MHC Class I H2-Db interacts with peptides derived from cytosolic proteins and loads them onto its structure. It forms a complex with beta-2 microglobulin and presents these peptides on the cell surface. This action is essential in signaling to CD8+ T cells which scan for non-self peptides originating from infections or cellular abnormalities. The method by which H2-Db binds peptides ensures precise immune surveillance.

Pathways

H2-Db operates prominently in the antigen processing and presentation pathway. This pathway plays an important role in immune response and involves proteases that generate peptide fragments. H2-Db interacts with transporter associated with antigen processing (TAP) proteins to bind peptides enabling effective display of antigens. Furthermore its activity connects with other immune proteins such as H2-Kb in presenting a comprehensive range of antigens to immune cells.

The involvement of H2-Db in autoimmune diseases and viral infections is significant. Certain autoimmune conditions arise when self-antigens are mistakenly presented triggering an immune response. Infections like viral hepatitis show how pathogens can manipulate H2-Db to evade immune detection. Tumor cells may also exploit H2-Db's antigen-presenting function. Proteins such as H2-Kb may interact similarly highlighting the need for MHC Class I diversity in combating disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the presentation of foreign antigens to the immune system.

See full target information H2-D1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 15:179-201 PubMed39354883

2024

TIMP1 Mediates Astrocyte-Dependent Local Immunosuppression in Brain Metastasis Acting on Infiltrating CD8+ T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Neibla Priego,Ana de Pablos-Aragoneses,María Perea-García,Valentina Pieri,Carolina Hernández-Oliver,Laura Álvaro-Espinosa,Andrea Rojas,Oliva Sánchez,Ariane Steindl,Eduardo Caleiras,Fernando García,Santiago García-Martín,Osvaldo Graña-Castro,Sandra García-Mulero,Diego Serrano,Paloma Velasco-Beltrán,Borja Jiménez-Lasheras,Leire Egia-Mendikute,Luise Rupp,Antonia Stammberger,Matthias Meinhardt,Anas Chaachou-Charradi,Elena Martínez-Saez,Luca Bertero,Paola Cassoni,Luca Mangherini,Alessia Pellerino,Roberta Rudà,Riccardo Soffietti,Fatima Al-Shahrour,Paul Saftig,Rebeca Sanz-Pamplona,Marc Schmitz,Stephen J Crocker,Alfonso Calvo,Asís Palazón,Manuel Valiente

Theranostics 13:2226-2240 PubMed37153743

2023

Analgesic and potentiated photothermal therapy with ropivacaine-loaded hydrogels.

Applications

Unspecified application

Species

Unspecified reactive species

Jiqian Zhang,Shasha Zhu,Mingxu Zhao,Mengni Zhou,Xiaoling Zhu,Xin Qing,Zhilai Yang,Pengfei Wei,Guoying Zhang,Weiling He,Yongqiang Yu,Xuesheng Liu

The Journal of clinical investigation 129:518-530 PubMed30422820

2018

Type I IFN blockade uncouples immunotherapy-induced antitumor immunity and autoimmune toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Scott R Walsh,Donald Bastin,Lan Chen,Andrew Nguyen,Christopher J Storbeck,Charles Lefebvre,David Stojdl,Jonathan L Bramson,John C Bell,Yonghong Wan

Journal of immunology (Baltimore, Md. : 1950) 132:751-8 PubMed6690617

1984

The interaction of monoclonal antibodies with MHC class I antigens on mouse spleen cells. I. Analysis of the mechanism of binding.

Applications

Unspecified application

Species

Unspecified reactive species

S K Dower,K Ozato,D M Segal

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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