AB281904

Anti-MHC class I antibody [R1-21.2]

Recombinant
What is this?

(1 Review)

(4 Publications)

Anti-MHC class I antibody [R1-21.2] (ab281904) is a rat monoclonal antibody detecting MHC class I in Western Blot, ICC/IF. Suitable for Mouse.

- Recombinant format for unrivalled batch-batch consistency

View Alternative Names

H2-K, H-2D(K), H2-D1, H-2D(B), H2-D1, H2-T23, H-2D(D), H2-D1, H2-K, H-2K(B), H2-K, H-2K(Q), MHC thymus leukemia antigen, H-2D(P), QA-2 antigen, Clone PAG64, H2-K, H2-K1, H-2K(D)

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MHC class I antibody [R1-21.2] (AB281904)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MHC class I antibody [R1-21.2] (AB281904)

This data was developed using the rabbit chimeric version (ab281902).

Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes labelling MHC-I with ab281902 at 10 μg/ml, followed by Alexa Fluor^® 488 secondary antibody at 2 μg/ml (green/ top-left). Image showing membrane staining in a subset of cells. The nuclear counter stain is DAPI (blue). The isotype control was anti-Fluorescein antibody followed by Alexa Fluor^® 488 secondary antibody (bottom-right). Mouse splenocytes were immobilized on Shi-fix™ cover-slips.

Supplier Data

Western blot - Anti-MHC class I antibody [R1-21.2] (AB281904)

This data was developed using the rabbit chimeric version (ab281902).

Lane 1:

Western blot - Anti-MHC class I antibody [R1-21.2] (ab281904) at 1 µg/mL

Lane 1:

Western blot - Anti-MHC class I antibody [R1-21.2] - Rabbit IgG (Chimeric) (<a href='/en-us/products/primary-antibodies/mhc-class-i-antibody-r1-212-rabbit-igg-chimeric-ab281902'>ab281902</a>) at 1 µg/mL

All lanes:

Mouse thymus tissue lysate at 35 µg

Secondary

All lanes:

Anti-rabbit secondary antibody

Predicted band size: 41 kDa

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

R1-21.2

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

WB, ICC/IF

applications

Immunogen

Cell preparation containing H2-Q9 protein. The exact immunogen used to generate this antibody is proprietary information.

P14431

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-MHC class I antibody [R1-21.2] (ab281904) is a rat recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Other related products
We have a range of other formats of antibody clone [R1-21.2] also available for your convenience: ab281902, ab281904

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MHC class I also known as MHC-1 or simply MHC I is a molecule playing an important role in the immune system. The molecule is a heterodimer composed of a heavy chain weighing around 45 kDa and a light chain. MHC class I molecules are expressed on nearly all nucleated cells in the body. They present peptide fragments from intracellular proteins to CD8+ T cells helping the immune system recognize and destroy infected or cancerous cells. Alternate names like H-2Kd/H-2Dd in mice highlight its role in antigen presentation across different species.

Biological function summary

The molecules of MHC class I form part of the adaptive immune system. They act as a complex with peptides which are derived from proteins degraded by the proteasome. The peptide-MHC I complex travels to the cell surface where it engages with the T cell receptor (TCR) on cytotoxic T cells. This interaction is essential for immune surveillance ensuring the body identifies cells that might compromise health such as those infected by viruses or transformed malignantly.

Pathways

The function of MHC class I integrates into the antigen processing and presentation pathways. It is pivotal in the proteasome-dependent pathway for processing endogenous antigens. Proteins like TAP (transporter associated with antigen processing) assist in transporting peptides into the endoplasmic reticulum for loading onto MHC class I. This pathway is central to cellular immunity and intersects with various immune response cascades.

MHC class I has associations with autoimmune disorders and certain viral infections. Aberrant expression or mutations can lead to diseases like Ankylosing Spondylitis or impact responses to chronic viral infections such as HIV. The involvement of MHC class I in presenting viral peptides often links it to immune evasion mechanisms utilizable by viruses. Hence this protein works closely with immune system proteins like cytokines influencing disease outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the presentation of foreign antigens to the immune system.

See full target information H2-Q9

Additional targets

H2-Q8,H2-Q10,H2-T23,H2-D1,H2-L,H2-K1,H2-Q7,H2-D1,H2-D1,H2-D1,H2-T3,H2-D1,H2-K1,,H2-K1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 32: PubMed40539438

2025

circRNA hsa_circ_0072107 aggravates myocardial hypertrophy via its function as a competitive endogenous RNA of miR‑516b‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Yongli He,Wuxia Ma,Jindong Xu,Qi Zhong,Cheng Huang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2500825 PubMed39965083

2025

Restoring Tumor Cell Immunogenicity Through Ion-Assisted p53 mRNA Domestication for Enhanced In Situ Cancer Vaccination Effect.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liang,Jingge Zhang,Jinjin Wang,Yuhe Yang,Xinyu Tan,Shuguang Li,Zhenzhen Guo,Zhenzhong Zhang,Junjie Liu,Jinjin Shi,Kaixiang Zhang

Molecular cancer therapeutics 24:105-117 PubMed39382073

2024

Tumor-Specific Antigen Delivery for T-cell Therapy via a pH-Sensitive Peptide Conjugate.

Applications

Unspecified application

Species

Unspecified reactive species

Annali M Yurkevicz,Yanfeng Liu,Samuel G Katz,Peter M Glazer

Frontiers in neurology 14:1269862 PubMed38107649

2023

Ursolic acid derivative UAOS-Na treats experimental autoimmune encephalomyelitis by immunoregulation and protecting myelin.

Applications

Unspecified application

Species

Unspecified reactive species

Maolin Wang,Chenming Gu,Yifu Yang,Liang Chen,Kaixian Chen,Jun Du,Huali Wu,Yiming Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MHC class I antibody [R1-21.2]

Immunocytochemistry/ Immunofluorescence - Anti-MHC class I antibody [R1-21.2] (AB281904)

Western blot - Anti-MHC class I antibody [R1-21.2] (AB281904)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (4)

Product promise