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AB281902

Anti-MHC class I antibody [R1-21.2] - Rabbit IgG (Chimeric)

3

(1 Review)

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(2 Publications)

Anti-MHC class I antibody [R1-21.2] - Rabbit IgG (Chimeric) (ab281902) is a rabbit monoclonal antibody detecting MHC class I in Western Blot. Suitable for Mouse.

- Recombinant format for unrivalled batch-batch consistency
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MHC class I antibody [R1-21.2] - Rabbit IgG (Chimeric) (AB281902)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MHC class I antibody [R1-21.2] - Rabbit IgG (Chimeric) (AB281902)

Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes labelling MHC-I with ab281902 at 10 μg/ml, followed by Alexa Fluor® 488 secondary antibody at 2 μg/ml (green/ top-left). Image showing membrane staining in a subset of cells. The nuclear counter stain is DAPI (blue). The isotype control was anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody (bottom-right). Mouse splenocytes were immobilized on Shi-fix™ cover-slips.

Western blot - Anti-MHC class I antibody [R1-21.2] - Rabbit IgG (Chimeric) (AB281902)
  • WB

Supplier Data

Western blot - Anti-MHC class I antibody [R1-21.2] - Rabbit IgG (Chimeric) (AB281902)

Primary incubation of 1h was used and protein was detected by chemiluminescence.

Lane 1:

Western blot - Anti-MHC class I antibody [R1-21.2] (<a href='/en-us/products/primary-antibodies/mhc-class-i-antibody-r1-212-ab281904'>ab281904</a>) at 1 µg/mL

Lane 1:

Western blot - Anti-MHC class I antibody [R1-21.2] - Rabbit IgG (Chimeric) (ab281902) at 1 µg/mL

All lanes:

Mouse thymus tissue lysate at 35 µg

Secondary

All lanes:

Anti-rabbit secondary antibody

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

R1-21.2

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

ICC, WB

applications

Immunogen

Cell preparation containing H2-Q9 protein. The exact immunogen used to generate this antibody is proprietary information.

P14431

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 mg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 mg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-MHC class I antibody [R1-21.2] - Rabbit IgG (Chimeric) (ab281902) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Mouse samples.

Other related products
We have a range of other formats of antibody clone [R1-21.2] also available for your convenience: ab281902, ab281904

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MHC class I also known as MHC-1 or simply MHC I is a molecule playing an important role in the immune system. The molecule is a heterodimer composed of a heavy chain weighing around 45 kDa and a light chain. MHC class I molecules are expressed on nearly all nucleated cells in the body. They present peptide fragments from intracellular proteins to CD8+ T cells helping the immune system recognize and destroy infected or cancerous cells. Alternate names like H-2Kd/H-2Dd in mice highlight its role in antigen presentation across different species.
Biological function summary

The molecules of MHC class I form part of the adaptive immune system. They act as a complex with peptides which are derived from proteins degraded by the proteasome. The peptide-MHC I complex travels to the cell surface where it engages with the T cell receptor (TCR) on cytotoxic T cells. This interaction is essential for immune surveillance ensuring the body identifies cells that might compromise health such as those infected by viruses or transformed malignantly.

Pathways

The function of MHC class I integrates into the antigen processing and presentation pathways. It is pivotal in the proteasome-dependent pathway for processing endogenous antigens. Proteins like TAP (transporter associated with antigen processing) assist in transporting peptides into the endoplasmic reticulum for loading onto MHC class I. This pathway is central to cellular immunity and intersects with various immune response cascades.

MHC class I has associations with autoimmune disorders and certain viral infections. Aberrant expression or mutations can lead to diseases like Ankylosing Spondylitis or impact responses to chronic viral infections such as HIV. The involvement of MHC class I in presenting viral peptides often links it to immune evasion mechanisms utilizable by viruses. Hence this protein works closely with immune system proteins like cytokines influencing disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the presentation of foreign antigens to the immune system.
See full target information H2-Q9

Additional targets

H2-Q8,H2-Q10,H2-T23,H2-D1,H2-L,H2-K1,H2-Q7,H2-D1,H2-D1,H2-D1,H2-T3,H2-D1,H2-K1,,H2-K1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of the science of food and agriculture 104:2059-2072 PubMed37917744

2023

L-Theanine alleviates heat stress through modulation of gut microbiota and immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Liu,Bin Wang,Ling Lin,Wei Xu,Zhi-Hua Gong,Wen-Jun Xiao

Cancer science 112:4444-4456 PubMed34382294

2021

WEE1 inhibitor and ataxia telangiectasia and RAD3-related inhibitor trigger stimulator of interferon gene-dependent immune response and enhance tumor treatment efficacy through programmed death-ligand 1 blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Wu,Xiaoyan Kang,Xiaoxiao Zhang,Wan Xie,Yue Su,Xiaoyu Liu,Lili Guo,Ensong Guo,Fuxia Li,Dianxing Hu,Xu Qin,Yu Fu,Wenju Peng,Jiedong Jia,Changyu Wang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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