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AB281903

Anti-MHC class I antibody [R1-9.6]

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(4 Publications)

Rat Recombinant Monoclonal HA19 antibody. Suitable for ICC/IF and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing H2-Q9 protein.
1 Images
Immunocytochemistry/ Immunofluorescence - Anti-MHC class I antibody [R1-9.6] (AB281903)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MHC class I antibody [R1-9.6] (AB281903)

This data was developed using the chimeric version (ab281901).

Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes labelling MHC-I with ab281901 at 10 μg/ml, followed by Alexa Fluor® 488 secondary antibody at 2 μg/ml (green/ top-left). Image showing membrane staining in a subset of cells. The nuclear counter stain is DAPI (blue). The isotype control was anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody. Mouse splenocytes were immobilized on Shi-fix™ cover-slips.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

R1-9.6

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

Cell preparation containing H2-Q9 protein. The exact immunogen used to generate this antibody is proprietary information.

P14431

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab281903 is the recombinant rat version of ab281901.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MHC class I also known as MHC-1 or simply MHC I is a molecule playing an important role in the immune system. The molecule is a heterodimer composed of a heavy chain weighing around 45 kDa and a light chain. MHC class I molecules are expressed on nearly all nucleated cells in the body. They present peptide fragments from intracellular proteins to CD8+ T cells helping the immune system recognize and destroy infected or cancerous cells. Alternate names like H-2Kd/H-2Dd in mice highlight its role in antigen presentation across different species.
Biological function summary

The molecules of MHC class I form part of the adaptive immune system. They act as a complex with peptides which are derived from proteins degraded by the proteasome. The peptide-MHC I complex travels to the cell surface where it engages with the T cell receptor (TCR) on cytotoxic T cells. This interaction is essential for immune surveillance ensuring the body identifies cells that might compromise health such as those infected by viruses or transformed malignantly.

Pathways

The function of MHC class I integrates into the antigen processing and presentation pathways. It is pivotal in the proteasome-dependent pathway for processing endogenous antigens. Proteins like TAP (transporter associated with antigen processing) assist in transporting peptides into the endoplasmic reticulum for loading onto MHC class I. This pathway is central to cellular immunity and intersects with various immune response cascades.

MHC class I has associations with autoimmune disorders and certain viral infections. Aberrant expression or mutations can lead to diseases like Ankylosing Spondylitis or impact responses to chronic viral infections such as HIV. The involvement of MHC class I in presenting viral peptides often links it to immune evasion mechanisms utilizable by viruses. Hence this protein works closely with immune system proteins like cytokines influencing disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the presentation of foreign antigens to the immune system.
See full target information H2-Q9

Additional targets

H2-Q8,H2-Q10,H2-T23,H2-D1,H2-L,H2-K1,H2-Q7,H2-D1,H2-D1,H2-D1,H2-T3,H2-D1,H2-K1,,H2-K1,H2-K1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1611637 PubMed40693271

2025

Antagonizing IL-6 receptor restores pancreatic tissue resident NK cells activation and ameliorates pancreatic injury in the mouse model of MASH.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Diana Abu Arra,Ahmad Salhab,Faris Kayed,Muneer Maali,Raghad Shweiki,Mustafa Ghanim

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2500825 PubMed39965083

2025

Restoring Tumor Cell Immunogenicity Through Ion-Assisted p53 mRNA Domestication for Enhanced In Situ Cancer Vaccination Effect.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liang,Jingge Zhang,Jinjin Wang,Yuhe Yang,Xinyu Tan,Shuguang Li,Zhenzhen Guo,Zhenzhong Zhang,Junjie Liu,Jinjin Shi,Kaixiang Zhang

Cancer letters 608:217326 PubMed39547332

2024

Towards melanoma in situ vaccination with multiple ultra-narrow X-ray beams.

Applications

Unspecified application

Species

Unspecified reactive species

Verdiana Trappetti,Cristian Fernández-Palomo,Prateek Arora,Marine Potez,Paolo Pellicioli,Jennifer Fazzari,Nahoko Shintani,Ismael Sanchez-Gonzalez,Cheuk Ting Wu,Bettina de Breuyn Dietler,Nadia Mercader-Huber,Olga A Martin,Stephan von Gunten,Vladislav Volarevic,Valentin Djonov

Cancer letters 529:37-52 PubMed34974132

2022

LC3 and NLRC5 interaction inhibits NLRC5-mediated MHC class I antigen presentation pathway in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhan,Junhui Zhang,Jing Zhang,Xiaojing Liu,Suding Zhu,Yuchuan Shi,Yu He,Wenyan Wang,Yijun Fan,Zhenhai Tang,Guo Chen,Bing Wei,Yunxia Cao
View all publications
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