AB33752

Anti-MHC class I RT Ia antibody [OX-18]

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(4 Publications)

Mouse Monoclonal MHC class I RT Ia antibody. Suitable for IP, Flow Cyt, ELISA, IHC-Fr and reacts with Rat samples. Cited in 4 publications.

View Alternative Names

RT1 class I gene, RT1 class I gene (A 1), RT1 class Ia locus A, RT1 class Ia, locus A (A1), RT1 class Ia, locus A1, RT1.A1(N), RT1A 1

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OX-18

Isotype

IgG1

Carrier free

Reacts with

Rat

Applications

IP, Flow Cyt, IHC-Fr, ELISA

applications

Specificity

Ab33752 recognises a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognised.

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Reactivity data

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Product details

This clone (OX-18) has been used in immunoaffinity purification of rat MHC class I molecules.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

This antibody was purified tissue culture supernatant.

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MHC class I RT Ia also known as RT1-A1 mechanically presents peptide antigens to CD8+ T cells. This protein has a mass of approximately 45 kDa. Expression of this protein occurs widely across various cell types including most nucleated cells. It plays an important role in the immune surveillance mechanism by displaying antigenic peptides on the cell surface for recognition by T-cell receptors.

Biological function summary

MHC class I RT Ia interacts with peptides within the endoplasmic reticulum and forms a complex with beta-2 microglobulin. This interaction is essential for the stability of the peptide-MHC complex on the cell surface. The formation of these complexes allows for immune recognition and establishment of immune responses. MHC class I RT Ia therefore plays an important role in initiating immune reactions against intracellular pathogens such as viruses.

Pathways

MHC class I RT Ia participates in the antigen processing and presentation pathway a fundamental aspect of adaptive immunity. It interacts with the transporter associated with antigen processing (TAP) proteins which transport peptides to the endoplasmic reticulum. Apart from that it also engages in the pathway involving cytotoxic T lymphocyte activation where it directly influences T-cell receptor signaling.

MHC class I RT Ia connects to autoimmune diseases like type 1 diabetes and ankylosing spondylitis. Variations in the genes encoding for MHC class I molecules may predispose individuals to these conditions. Additionally it’s associated with the HIV-1 virus where the virus may escape immune detection by downregulating MHC class I molecules presenting challenges in immune response which affect viral loads and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MHC class I RT Ia

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Experimental neurology 282:78-85 PubMed27235932

2016

hESC-derived neural progenitors prevent xenograft rejection through neonatal desensitisation.

Applications

IHC

Species

Rat

Andreas Heuer,Agnete Kirkeby,Ulrich Pfisterer,Marie E Jönsson,Malin Parmar

Immunology letters 135:118-23 PubMed20974178

2010

Major histocompatibility complex class I-mediated inhibition of neurite outgrowth from peripheral nerves.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongqi-Phyllis Wu,Tina Bilousova,Nathalie Escande-Beillard,Hoa Dang,Terry Hsieh,Jide Tian,Daniel L Kaufman

Transplantation proceedings 25:2806-7 PubMed8212242

1993

Preincubation of tumor cells with the anti-(MHC) class I antibody OX18 enhances tumor cell lysis by interleukin-2-activated NK cells.

Applications

Unspecified application

Species

Unspecified reactive species

K M Smits,P J Kuppen,A M Eggermont,G J Fleuren

European journal of immunology 12:237-43 PubMed6178598

1982

Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus.

Applications

Unspecified application

Species

Unspecified reactive species

T Fukumoto,W R McMaster,A F Williams

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MHC class I RT Ia antibody [OX-18]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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