Anti-MHC Class II antibody [6C6]

• IHC-P

AbReview25380****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II antibody [6C6] (AB55152)

ab55152 staining MHC Class II in Human bowel tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1/50 in milk) for 21 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/300) was used as the secondary antibody.

This image was generated using the ascites version of the product.

This image is courtesy of an anonymous Abreview

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II antibody [6C6] (AB55152)

MHC Class II antibody (ab55152) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lymph node.

This image was generated using the ascites version of the product.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MHC Class II antibody [6C6] (AB55152)

ICC/IF image of ab55152 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab55152 at 10μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

This image was generated using the ascites version of the product.

• Flow Cyt

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Flow Cytometry - Anti-MHC Class II antibody [6C6] (AB55152)

Overlay histogram showing Raji cells stained with ab55152 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55152, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This image was generated using the ascites version of the product.

• WB

Supplier Data

Western blot - Anti-MHC Class II antibody [6C6] (AB55152)

Western blot against tagged recombinant protein immunogen using ab55152 MHC Class II antibody at 1ug/ml. Predicted band size of immunogen is 54 kDa

All lanes:

Western blot - Anti-MHC Class II antibody [6C6] (ab55152)

Predicted band size: 29 kDa

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• WB

Supplier Data

Western blot - Anti-MHC Class II antibody [6C6] (AB55152)

All lanes:

Western blot - Anti-MHC Class II antibody [6C6] (ab55152)

Lane 1:

MHC Class II transfected 293T cell lysates

Lane 2:

Non-transfected 293T cell lysates

Predicted band size: 29 kDa

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