Skip to main content

Mouse Recombinant Monoclonal CD74 antibody. Carrier free. Suitable for IHC-P, ICC/IF and reacts with Rat, Mouse samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II antibody [MRC OX-6] - BSA and Azide free (AB237959), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II antibody [MRC OX-6] - BSA and Azide free (AB237959), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MHC Class II antibody [MRC OX-6] - BSA and Azide free (AB237959), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MHC Class II antibody [MRC OX-6] - BSA and Azide free (AB237959), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Not recommended
Not recommended
Mouse
Not recommended
Tested
Rat
Tested
Tested

Tested
Tested

Species

Rat

Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place. Enhance also the stimulation of T-cell responses through interaction with CD44.Class-II-associated invariant chain peptideBinds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.Isoform LongStabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs).

Alternative names

CD74, H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, MHC class II-associated invariant chain, Ii

Recommended products

Mouse Recombinant Monoclonal CD74 antibody. Carrier free. Suitable for IHC-P, ICC/IF and reacts with Rat, Mouse samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

MRC OX-6

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab237959 is the carrier-free version of Anti-MHC Class II antibody [MRC OX-6] ab23990.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MHC Class II also known as MHC II or human leukocyte antigen (HLA) class II is a protein complex with a molecular mass generally around 55 kDa. This protein is mainly expressed on the surface of antigen-presenting cells such as dendritic cells macrophages and B cells. It plays an important role in the immune system by presenting extracellular antigens to T helper cells. MHC II molecules are composed of two polypeptide chains alpha and beta which associate to form a heterodimer on the cell surface.

Biological function summary

MHC Class II molecules facilitate the immune system's recognition and response to pathogens by presenting processed antigenic peptides to CD4+ T lymphocytes. This activity involves their assembly within the endosomal-lysosomal system where they bind peptides derived from endocytosed proteins. MHC II is part of the major histocompatibility complex which includes other related molecules such as MHC Class I (HLA class I). The binding and presentation of peptides are central for the coordination of adaptive immune responses enhancing pathogen clearance and maintaining immunological memory.

Pathways

MHC Class II operates within the antigen processing and presentation pathway important for adaptive immunity. Its interaction with CD4+ T cells stimulates downstream signaling pathways leading to T cell activation and differentiation. Related proteins in the antigen presentation pathway include invariant chain (Ii) which guides MHC II to the endosome and HLA-DM which facilitates peptide loading. Together these proteins and pathways work to ensure effective immune surveillance and response.

Associated diseases and disorders

Alterations in MHC Class II expression or function are associated with autoimmune diseases such as rheumatoid arthritis and type 1 diabetes. In these conditions improper antigen presentation may lead to an inappropriate immune response against self-proteins. The involvement of MHC II in disease is often linked to specific HLA alleles which vary among individuals and influence susceptibility to autoimmune disorders. Additionally the abnormal function of connected proteins like cytokines from activated T helper cells can exacerbate these immune conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II antibody [MRC OX-6] - BSA and Azide free (ab237959), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United Kingdom

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II antibody [MRC OX-6] - BSA and Azide free (ab237959)

    Immunohistochemistical detection of MHC Class II using antibody Anti-MHC Class II antibody [MRC OX-6] ab23990 on PFA-fixed rat spleen tissue sections. Antibody diluted at 1/100 and incubated for2 hours in TBS/BSA/Tween/azide. Secondary antibody: anti mouse IgG conjugated to biotin (1/100). After dissection of spleen from PFA-perfused specimen it was sampled and further immersion-fixed for two Hrs. After subsequent immersion in 30% Sucrose, specimens were snap-frozen. Before immunostaining, the 8 micron sections were placed in a 60 degree C oven for 60 mins to enhance adhesion. The submitted image shows white pulp (PALS) and a small area of red pulp (RP-upper left). The Periarteriolar Sheath (PALS) with it's Central Arteriole/artery (CA)shows many positive cells (B-lymphocytes and Macrophages) and negative lymphocytes (T-cells?). The Marginal Sinus (MS) is clearly seen between the PALS and the Marginal Zone (MZ). There is a clear Germinal Centre (GS) in this image.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (Anti-MHC Class II antibody [MRC OX-6] ab23990).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II antibody [MRC OX-6] - BSA and Azide free (ab237959), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II antibody [MRC OX-6] - BSA and Azide free (ab237959)

    IHC image of MHC Class II staining in Rat normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-MHC Class II antibody [MRC OX-6] ab23990, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (Anti-MHC Class II antibody [MRC OX-6] ab23990).

  • Immunocytochemistry/ Immunofluorescence - Anti-MHC Class II antibody [MRC OX-6] - BSA and Azide free (ab237959), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MHC Class II antibody [MRC OX-6] - BSA and Azide free (ab237959)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (Anti-MHC Class II antibody [MRC OX-6] ab23990).

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocyte cells labelling MHC Class II with Anti-MHC Class II antibody [MRC OX-6] ab23990 at 1/100 dilution (10.63 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used at 1/100 dilution (5µg/mL) as counterstain for tubulin (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody.

    Confocal image showing membranous and cytoplasmic staining in subsets of mouse splenocyte . Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-MHC Class II antibody [MRC OX-6] - BSA and Azide free (ab237959), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MHC Class II antibody [MRC OX-6] - BSA and Azide free (ab237959)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (Anti-MHC Class II antibody [MRC OX-6] ab23990).

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat splenocyte cells labelling MHC Class II with Anti-MHC Class II antibody [MRC OX-6] ab23990 at 1/100 dilution (10.63 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used at 1/100 dilution (5µg/mL) as counterstain for tubulin (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody.

    Confocal image showing membranous and cytoplasmic staining in subsets of rat splenocyte.
    Negative control: C6.
    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com