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AB25681

Anti-MHC class II I E kappa antibody [14-4-4S]

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(8 Publications)

Mouse Monoclonal HB2J antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 8 publications. Immunogen corresponding to Tissue preparation containing MHC class II I E kappa protein.

View Alternative Names

H2-Eb1

1 Images
Flow Cytometry - Anti-MHC class II I E kappa antibody [14-4-4S] (AB25681)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MHC class II I E kappa antibody [14-4-4S] (AB25681)

Flow Cytometry analysis of AKR mouse splenocytes stained for MHC class II I E kappa using ab25681 (1 μg/106 cells) and a Rat Anti-Mouse CD3ε-PE followed by a Goat Anti-Mouse IgG2a, Human ads-FITC.

  • 578 PE

    PE Anti-MHC class II I E kappa antibody [14-4-4S]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

14-4-4S

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to an epitope on mouse I-E kappa MHC class II alloantigen. The antibody reacts with the I-E kappa class II alloantigen on cells from mice of the H-2<sup>d</sup>, H-2<sup>p</sup>, and H-2<sup>r</sup> haplotypes. The antibody has also been reported to cross react with the rat class II alloantigen RT1D.

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Primary Antibodies

AB23990

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  • 6
Primary Antibodies

AB307523

Alexa Fluor® 647 Anti-CD11b antibody [EPR1344]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 8.2 Constituents: 100% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MHC class II I-E kappa also known as I-Ek is a protein involved in the immune system's antigen-presenting function. It is part of the major histocompatibility complex (MHC) class II molecules which are important for presenting exogenous antigens to CD4+ T helper cells. MHC class II molecules have a molecular mass that ranges from 50 to 70 kDa. I-Ek is usually expressed on the surface of professional antigen-presenting cells like dendritic cells macrophages and B cells.
Biological function summary

I-Ek is involved in the immune response by forming a complex with peptides derived from external sources. This complex is then presented on the cell surface to facilitate recognition by CD4+ T helper cells which play a critical role in adaptive immunity. I-Ek's interactions are essential for triggering T cell-mediated immune responses important in fighting infections and maintaining immune tolerance. Researchers widely study this protein to understand immune response modulation.

Pathways

MHC class II I-Ek is a component of the antigen processing and presentation pathway critical in adaptive immune responses. Within this pathway the I-Ek protein interacts with the invariant chain and memory B cells to assist in antigen presentation. It also participates in the intricate network where it communicates with CD4+ receptor-bearing T cells important for effective immune system activation.

MHC class II I-Ek has significant implications in autoimmune diseases such as rheumatoid arthritis and type 1 diabetes. Aberrant expression or malfunction of I-Ek can contribute to these conditions by promoting inappropriate immune responses against self-antigens. The protein interacts with other molecules like human leukocyte antigen (HLA) linked to autoimmunity serving as potential research targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information H2-Eb1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature methods 22:1707-1719 PubMed40721872

2025

Sliding Window Interaction Grammar (SWING): a generalized interaction language model for peptide and protein interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Jane C Siwek,Alisa A Omelchenko,Prabal Chhibbar,Sanya Arshad,AnnaElaine Rosengart,Iliyan Nazarali,Akash Patel,Kiran Nazarali,Javad Rahimikollu,Jeremy S Tilstra,Mark J Shlomchik,David R Koes,Alok V Joglekar,Jishnu Das

Signal transduction and targeted therapy 8:236 PubMed37332010

2023

Soluble CD4 effectively prevents excessive TLR activation of resident macrophages in the onset of sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Yuan Zhang,Qiu-Ping Xu,Li-Na Shi,Shih-Wen Li,Wei-Hong Wang,Qing-Qing Wang,Liao-Xun Lu,Hui Xiao,Jun-Hong Wang,Feng-Ying Li,Yin-Ming Liang,Si-Tang Gong,Hao-Ran Peng,Zheng Zhang,Hong Tang

Frontiers in immunology 11:392 PubMed32184790

2020

Identification and Characterization of Adipose Tissue-Derived Human Antibodies With "Anti-self" Specificity.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Frasca,Alain Diaz,Maria Romero,Denisse Garcia,Diya Jayram,Seth Thaller,Maria Del Carmen Piqueras,Sanjoy Bhattacharya,Bonnie B Blomberg

European journal of immunology 26:3185-93 PubMed8977321

1996

Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells.

Applications

Unspecified application

Species

Unspecified reactive species

A Farr,P C DeRoos,S Eastman,A Y Rudensky

European journal of immunology 25:596-603 PubMed7533092

1995

Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses.

Applications

Unspecified application

Species

Unspecified reactive species

M Roy,A Aruffo,J Ledbetter,P Linsley,M Kehry,R Noelle

International immunology 6:61-71 PubMed8148327

1994

Non-coordinate synthesis of MHC class II proteins and invariant chains by epidermal Langerhans cells derived from short-term in vitro culture.

Applications

Unspecified application

Species

Unspecified reactive species

U Neiss,K Reske

Immunogenetics 34:358-65 PubMed1748486

1991

Structure function analysis of the H-2 Abp gene.

Applications

Unspecified application

Species

Unspecified reactive species

J A Harton,W Litaker,J A Frelinger,G A Bishop

Journal of immunology (Baltimore, Md. : 1950) 124:533-40 PubMed7188699

1980

Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens.

Applications

Unspecified application

Species

Unspecified reactive species

K Ozato,N Mayer,D H Sachs
View all publications
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