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AB72487

Anti-MHC Qa1b antibody [6A8.6F10.1A6]

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(2 Publications)

Mouse Monoclonal HA15 antibody. Suitable for WB, Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

H2 Q1, H2 T23, H2 class I histocompatibility antigen D37 alpha chain, Histocompatibility 2 T region locus 23, MHC class I antigen Qa1, MHC classIb Qa1, OTTMUSP00000015895, QA1

3 Images
Flow Cytometry - Anti-MHC Qa1b antibody [6A8.6F10.1A6] (AB72487)
  • Flow Cyt

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Flow Cytometry - Anti-MHC Qa1b antibody [6A8.6F10.1A6] (AB72487)

Flow cytometric analysis of MHC Qa1b in 72 hour ConA-stimulated Balb/c mouse splenocytes using A) isotype control and B) ab72487 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/106 cells. A shift is seen in the entire population of cells.

Flow Cytometry - Anti-MHC Qa1b antibody [6A8.6F10.1A6] (AB72487)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-MHC Qa1b antibody [6A8.6F10.1A6] (AB72487)

Flow cytometric analysis of MHC Qa1b in unstimulated Balb/c mouse splenocytes using A) isotype control and B) ab72487 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/106 cells. A shift is seen in only a small percentage of cells.

Western blot - Anti-MHC Qa1b antibody [6A8.6F10.1A6] (AB72487)
  • WB

Supplier Data

Western blot - Anti-MHC Qa1b antibody [6A8.6F10.1A6] (AB72487)

Western blot analysis of mouse thymus tissue lysate using ab72487 at 2 μg/ml.

All lanes:

Western blot - Anti-MHC Qa1b antibody [6A8.6F10.1A6] (ab72487)

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6A8.6F10.1A6

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

WB, Flow Cyt

applications

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1-0.5 µg/mL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

ab72487 can be used for flow cytometric assay to detect Qa1b on immuno-regulatory antigen presenting cells and activated splenic T cells on Qa1b expressing mice (e.g. BALB/c, C57BL/6) and it can also be used for functional assays to block target cell recognition by CTLs specific for Qa1 restricted antigen presentation and other functional study where Qa1b associated immuno-regulation may work.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MHC Qa1b also known as Qa-1b is a non-classical MHC class Ib molecule. It weighs approximately 45 kDa and belongs to the MHC class I family. Qa-1b is mainly expressed on the surface of lymphocytes as well as other nucleated cells. Its primary function includes the presentation of peptide antigens to CD8+ T cells facilitating immune surveillance. Qa-1b molecules possess a restricted tissue distribution compared to classical MHC molecules which makes them unique in their function and expression.
Biological function summary

Qa-1b plays an essential role in modulating immune responses. It interacts as a ligand with CD94/NKG2 receptors on natural killer (NK) cells and some T cells impacting the regulation of these cells’ activity. Through this interaction Qa-1b contributes to the inhibition or activation of NK cells influencing immune tolerance and the immune system’s response to infected or transformed cells. Qa-1b as part of a larger complex associates with β2-microglobulin to form a stable structure on the cell surface essential for its antigen-presenting function.

Pathways

Qa-1b contributes significantly to both the innate and adaptive immune pathways. By acting within the antigen presentation and NK cell activity pathways Qa-1b cooperates with other molecules involved in immune regulation such as MHC class I molecules and immunoreceptors like CD94/NKG2. This collaboration ensures proper immune system function by maintaining balance between immune surveillance and self-tolerance preventing autoimmunity.

Qa-1b has a potential connection to autoimmune diseases and cancer. Its role in maintaining immune tolerance makes it a critical factor in conditions like rheumatoid arthritis where immune regulation is disrupted. Qa-1b's interaction with CD94/NKG2 receptors can also influence tumor immunity as it might help cancer cells evade immune detection. Understanding Qa-1b’s function and interactions with other proteins in these contexts is important for developing therapeutic approaches targeting these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MHC Qa1b
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

European journal of immunology 46:929-40 PubMed26711740

2016

Microenvironmental stresses induce HLA-E/Qa-1 surface expression and thereby reduce CD8(+) T-cell recognition of stressed cells.

Applications

Unspecified application

Species

Unspecified reactive species

Takanori Sasaki,Takayuki Kanaseki,Yosuke Shionoya,Serina Tokita,Sho Miyamoto,Eri Saka,Vitaly Kochin,Akira Takasawa,Yoshihiko Hirohashi,Yasuaki Tamura,Akihiro Miyazaki,Toshihiko Torigoe,Hiroyoshi Hiratsuka,Noriyuki Sato

Proceedings of the National Academy of Sciences of 111:2704-9 PubMed24550298

2014

Ischemic neurons recruit natural killer cells that accelerate brain infarction.

Applications

ICC/IF

Species

Mouse

Yan Gan,Qiang Liu,Wei Wu,Jun-Xiang Yin,Xue-Feng Bai,Rulong Shen,Yongjun Wang,Jieli Chen,Antonio La Cava,Jennifer Poursine-Laurent,Wayne Yokoyama,Fu-Dong Shi
View all publications
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Product promise

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