Rabbit Polyclonal MIA3/TANGO1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MIA3.
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- Circulation research 127:1552-15652020Genetic Regulation of Atherosclerosis-Relevant Phenotypes in Human Vascular Smooth Muscle Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRedouane Aherrahrou,Liang Guo,V Peter Nagraj,Aaron Aguhob,Jameson Hinkle,Lisa Chen,Joon Yuhl Soh,Dillon Lue,Gabriel F Alencar,Arjan Boltjes,Sander W van der Laan,Emily Farber,Daniela Fuller,Rita Anane-Wae,Ngozi Akingbesote,Ani W Manichaikul,Lijiang Ma,Minna U Kaikkonen,Johan L M Björkegren,Suna Önengüt-Gümüşcü,Gerard Pasterkamp,Clint L Miller,Gary K Owens,Aloke Finn,Mohamad Navab,Alan M Fogelman,Judith A Berliner,Mete CivelekPubMed 33040646
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MIA3. The exact immunogen used to generate this antibody is proprietary information. Database link Q5JRA6
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
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2 products for Alternative Product
Target data
Function
Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed:19269366, PubMed:27138255). Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES (PubMed:28442536).
Alternative names
KIAA0268, TANGO, UNQ6077/PRO20088, MIA3, Transport and Golgi organization protein 1 homolog, TANGO1, C219-reactive peptide, D320, Melanoma inhibitory activity protein 3
Recommended products
Rabbit Polyclonal MIA3/TANGO1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MIA3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MIA3. The exact immunogen used to generate this antibody is proprietary information. Database link Q5JRA6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MIA3/TANGO1 also known as Melanoma Inhibitory Activity 3 or Transport and Golgi Organization 1 is a protein with a molecular mass of approximately 190 kDa. This protein mainly expresses in the endoplasmic reticulum (ER). It plays an essential role in the secretory pathway by mediating the export of large molecules such as collagen from the ER to the Golgi apparatus. MIA3/TANGO1 acts as a scaffold organizing COPII vesicle coating components to facilitate the correct packaging and export of secretory proteins.
- Biological function summary
MIA3/TANGO1 integrates into a larger ER export complex. This complex includes other proteins like cTAGE5 and Sec12 which assist in the proper assembly and budding of cargo-loaded vesicles. It maintains cellular homeostasis by ensuring cells correctly secrete ECM molecules such as collagen. Proper ECM molecule assembly and export are critical for maintaining tissue architecture particularly in connective tissues where collagen forms a substantial part of the structural matrix.
- Pathways
MIA3/TANGO1 participates in the collagen biosynthesis and trafficking pathway. It actively interacts with proteins like cTAGE5 and Sec23 which are key components in the COPII vesicle formation. By influencing these pathways it affects the overall dynamics of the secretory machinery contributing to the accurate delivery of structural proteins necessary for cell structure integrity. MIA3/TANGO1's activity impacts the efficiency of the ER-to-Golgi transport an essential transition within the secretory pathway.
- Associated diseases and disorders
MIA3/TANGO1's dysfunction links to connective tissue disorders and certain forms of cancer. Mutations in MIA3/TANGO1 impact collagen secretion efficiency leading to conditions like Ehlers-Danlos syndrome a connective tissue disorder. Its interactions with proteins involved in these diseases such as HSP47 (a collagen-specific chaperone) further highlight its pathological relevance. Abnormal MIA3/TANGO1 function also associates with tumorigenesis as its role in ECM regulation could influence the tumor microenvironment impacting cancer progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIA3/TANGO1 antibody (ab244506)
Formalin-fixed, paraffin-embedded human testis tissue stained for MIA3/TANGO1 with ab244506 in immunohistochemical analysis.
Western blot - Anti-MIA3/TANGO1 antibody (ab244506)
All lanes: Western blot - Anti-MIA3/TANGO1 antibody (ab244506) at 0.4 µg/mL
All lanes: U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 213 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MIA3/TANGO1 antibody (ab244506)
PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for MIA3/TANGO1 (green) using ab244506 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIA3/TANGO1 antibody (ab244506)
Formalin-fixed, paraffin-embedded human prostate tissue stained for MIA3/TANGO1 with ab244506 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIA3/TANGO1 antibody (ab244506)
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for MIA3/TANGO1 with ab244506 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIA3/TANGO1 antibody (ab244506)
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for MIA3/TANGO1 with ab244506 in immunohistochemical analysis.
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