AB97881

Anti-MIA3/TANGO1 antibody

Name: Anti-MIA3/TANGO1 antibody (ab97881) | Abcam
Brand: Abcam Limited
SKU: AB97881

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(2 Publications)

Rabbit Polyclonal MIA3/TANGO1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MIA3 aa 1-150.

View Alternative Names

KIAA0268, TANGO, UNQ6077/PRO20088, MIA3, Transport and Golgi organization protein 1 homolog, TANGO1, C219-reactive peptide, D320, Melanoma inhibitory activity protein 3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MIA3/TANGO1 antibody (AB97881)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MIA3/TANGO1 antibody (AB97881)

Immunofluorescence analysis of methanol fixed HepG2 cells, using ab97881 at a 1/200 dilution.
Lower image : costained with Hoechst 33342.

Supplier Data

Western blot - Anti-MIA3/TANGO1 antibody (AB97881)

5% SDS PAGE.

All lanes:

Western blot - Anti-MIA3/TANGO1 antibody (ab97881) at 1/1000 dilution

Lane 1:

A549 whole cell lysate at 30 µg

Lane 2:

Hep G2 whole cell lysate at 30 µg

Predicted band size: 213 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MIA3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q5JRA6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIA3/TANGO1 also known as Melanoma Inhibitory Activity 3 or Transport and Golgi Organization 1 is a protein with a molecular mass of approximately 190 kDa. This protein mainly expresses in the endoplasmic reticulum (ER). It plays an essential role in the secretory pathway by mediating the export of large molecules such as collagen from the ER to the Golgi apparatus. MIA3/TANGO1 acts as a scaffold organizing COPII vesicle coating components to facilitate the correct packaging and export of secretory proteins.

Biological function summary

MIA3/TANGO1 integrates into a larger ER export complex. This complex includes other proteins like cTAGE5 and Sec12 which assist in the proper assembly and budding of cargo-loaded vesicles. It maintains cellular homeostasis by ensuring cells correctly secrete ECM molecules such as collagen. Proper ECM molecule assembly and export are critical for maintaining tissue architecture particularly in connective tissues where collagen forms a substantial part of the structural matrix.

Pathways

MIA3/TANGO1 participates in the collagen biosynthesis and trafficking pathway. It actively interacts with proteins like cTAGE5 and Sec23 which are key components in the COPII vesicle formation. By influencing these pathways it affects the overall dynamics of the secretory machinery contributing to the accurate delivery of structural proteins necessary for cell structure integrity. MIA3/TANGO1's activity impacts the efficiency of the ER-to-Golgi transport an essential transition within the secretory pathway.

MIA3/TANGO1's dysfunction links to connective tissue disorders and certain forms of cancer. Mutations in MIA3/TANGO1 impact collagen secretion efficiency leading to conditions like Ehlers-Danlos syndrome a connective tissue disorder. Its interactions with proteins involved in these diseases such as HSP47 (a collagen-specific chaperone) further highlight its pathological relevance. Abnormal MIA3/TANGO1 function also associates with tumorigenesis as its role in ECM regulation could influence the tumor microenvironment impacting cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed : 19269366, PubMed : 27138255). Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES (PubMed : 28442536).

See full target information MIA3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastrointestinal oncology 12:52-68 PubMed33708424

2021

Exosomal miR-30a and miR-222 derived from colon cancer mesenchymal stem cells promote the tumorigenicity of colon cancer through targeting MIA3.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Du,Xuan Ye,Sheng-Rong Lu,Huan Li,Hong-Yue Liu,Qing Zhai,Bo Yu

PloS one 13:e0189711 PubMed29298329

2018

Molecular and functional analysis of anchorage independent, treatment-evasive neuroblastoma tumorspheres with enhanced malignant properties: A possible explanation for radio-therapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara J Abou-Antoun,Javad Nazarian,Anthony Ghanem,Stanislav Vukmanovic,Anthony D Sandler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MIA3/TANGO1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MIA3/TANGO1 antibody (AB97881)

Western blot - Anti-MIA3/TANGO1 antibody (AB97881)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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