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Rabbit Polyclonal MIA3/TANGO1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MIA3 aa 1-150.

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Images

Western blot - Anti-MIA3/TANGO1 antibody (AB97881), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MIA3/TANGO1 antibody (AB97881), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human MIA3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q5JRA6

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/200.00000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed:19269366, PubMed:27138255). Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES (PubMed:28442536).

Alternative names

Recommended products

Rabbit Polyclonal MIA3/TANGO1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MIA3 aa 1-150.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human MIA3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q5JRA6
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MIA3/TANGO1 also known as Melanoma Inhibitory Activity 3 or Transport and Golgi Organization 1 is a protein with a molecular mass of approximately 190 kDa. This protein mainly expresses in the endoplasmic reticulum (ER). It plays an essential role in the secretory pathway by mediating the export of large molecules such as collagen from the ER to the Golgi apparatus. MIA3/TANGO1 acts as a scaffold organizing COPII vesicle coating components to facilitate the correct packaging and export of secretory proteins.

Biological function summary

MIA3/TANGO1 integrates into a larger ER export complex. This complex includes other proteins like cTAGE5 and Sec12 which assist in the proper assembly and budding of cargo-loaded vesicles. It maintains cellular homeostasis by ensuring cells correctly secrete ECM molecules such as collagen. Proper ECM molecule assembly and export are critical for maintaining tissue architecture particularly in connective tissues where collagen forms a substantial part of the structural matrix.

Pathways

MIA3/TANGO1 participates in the collagen biosynthesis and trafficking pathway. It actively interacts with proteins like cTAGE5 and Sec23 which are key components in the COPII vesicle formation. By influencing these pathways it affects the overall dynamics of the secretory machinery contributing to the accurate delivery of structural proteins necessary for cell structure integrity. MIA3/TANGO1's activity impacts the efficiency of the ER-to-Golgi transport an essential transition within the secretory pathway.

Associated diseases and disorders

MIA3/TANGO1's dysfunction links to connective tissue disorders and certain forms of cancer. Mutations in MIA3/TANGO1 impact collagen secretion efficiency leading to conditions like Ehlers-Danlos syndrome a connective tissue disorder. Its interactions with proteins involved in these diseases such as HSP47 (a collagen-specific chaperone) further highlight its pathological relevance. Abnormal MIA3/TANGO1 function also associates with tumorigenesis as its role in ECM regulation could influence the tumor microenvironment impacting cancer progression.

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2 product images

  • Western blot - Anti-MIA3/TANGO1 antibody (ab97881), expandable thumbnail

    Western blot - Anti-MIA3/TANGO1 antibody (ab97881)

    5% SDS PAGE.

    All lanes: Western blot - Anti-MIA3/TANGO1 antibody (ab97881) at 1/1000 dilution

    Lane 1: A549 whole cell lysate at 30 µg

    Lane 2: Hep G2 whole cell lysate at 30 µg

    Predicted band size: 213 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MIA3/TANGO1 antibody (ab97881), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MIA3/TANGO1 antibody (ab97881)

    Immunofluorescence analysis of methanol fixed HepG2 cells, using ab97881 at a 1/200 dilution.
    Lower image: costained with Hoechst 33342.

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Product protocols

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