Anti-Mib1/Mindbomb antibody [EPR2762(2)]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (AB124929)

ab124929 staining Mib1/Mindbomb in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

This image was produced using purified antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (AB124929)

Immunohistochemical analysis of Human breast cancer tissue, staining Mib1/Mindbomb with ab124929.

Tissue was fixed with paraformaldehyde and permeabilized with Tween buffer for 30 minutes at 20°C; antigen retrieval was by heat mediation in Tris/EDTA buffer (pH 9). Samples were incubated with primary antibody (1/5000 in diluent) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Mr Rudolf Jung

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (AB124929)

ab124929 staining Mib1/Mindbomb in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab124929 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

Negative control 1 : Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2 : Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

This image was produced using purified antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (AB124929)

ab124929, at 1/250 dilution, staining Mib1/Mindbomb in formalin-fixed, paraffin-embedded Human kidney tissue by immunohistochemistry.

This image was produced using unpurified antibody.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

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Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (AB124929)

This image was produced using unpurified antibody.

All lanes:

Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/1000 dilution

Lane 1:

K562 cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Lane 3:

Caco2 cell lysates at 10 µg

Lane 4:

NCCIT cell lysates at 10 µg

Lane 5:

Human brain lysates at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 110 kDa

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• WB

Lab

Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (AB124929)

Blocking and diluting buffer : 5% NFDM/TBST

This image was produced using purified antibody.

All lanes:

Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/10000 dilution

Lane 1:

Human fetal brain tissue at 20 µg

Lane 2:

K562 (human chronic myelogenous leukemia) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

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• WB

Lab

Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (AB124929)

Diluting and blocking buffer : 5% NFDM /TBST.

This image was produced using purified antibody.

All lanes:

Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/1000 dilution

All lanes:

Mouse spleen tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

false

• WB

Lab

Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (AB124929)

Blocking and diluting buffer : 5% NFDM/TBST.

This image was produced using purified antibody.

All lanes:

Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/10000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 110 kDa

false

• OI-RD Scanning

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OI-RD Scanning - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (AB124929)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.