Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (AB245766)

ab124929 staining Mib1/Mindbomb in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab124929 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain. **Negative control 1** : Rabbit primary antibody and anti-mouse secondary antibody (ab150120) **Negative control 2 : ** Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) This image was produced using purified antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab124929).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (AB245766)

ab124929, at 1/250 dilution, staining Mib1/Mindbomb in formalin-fixed, paraffin-embedded Human kidney tissue by immunohistochemistry.

This image was produced using unpurified antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab124929).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (AB245766)

Immunohistochemical analysis of Human breast cancer tissue, staining Mib1/Mindbomb with ab124929.

Tissue was fixed with paraformaldehyde and permeabilized with Tween buffer for 30 minutes at 20°C; antigen retrieval was by heat mediation in Tris/EDTA buffer (pH 9). Samples were incubated with primary antibody (1/5000 in diluent) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; glycerol and sodium azide (ab124929)

• IHC-P

AbReview32727****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (AB245766)

Immunohistochemical analysis of Human breast cancer tissue, staining Mib1/Mindbomb with ab124929.

Tissue was fixed with paraformaldehyde and permeabilized with Tween buffer for 30 minutes at 20°C; antigen retrieval was by heat mediation in Tris/EDTA buffer (pH 9). Samples were incubated with primary antibody (1/5000 in diluent) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab124929).

This image is courtesy of an Abreview submitted by Mr Rudolf Jung

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (AB245766)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.