AB54413

Anti-MICA + MICB antibody [6D4]

Recombinant
What is this?

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(4 Publications)

Mouse Recombinant Monoclonal MICA antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications.

View Alternative Names

PERB11.1, MICA, MHC class I polypeptide-related sequence A, MIC-A

1 Images

Flow Cytometry - Anti-MICA + MICB antibody [6D4] (AB54413)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-MICA + MICB antibody [6D4] (AB54413)

Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / HeLa (Human cervix adenocarcinoma epithelial cell, Right) cells labelling MICA + MICB with ab54413 at 1/1000 dilution (0.1μg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

Negative control : Jurkat.

Gated on viable cells.

Carrier free

Anti-MICA + MICB antibody [6D4] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6D4

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MICA and MICB also known as MIC-A and MIC-B are stress-induced proteins with significant roles in immune responses. These proteins are widely known for their presence on the surface of many human cell types most notably epithelial and endothelial cells. MICA is a glycoprotein with a molecular mass of approximately 70 kDa while MICB has a similar structure and mass. They are both part of the MHC class I-like molecules and function as ligands to the NKG2D receptor on natural killer (NK) cells and certain T cells a critical interaction for immune surveillance.

Biological function summary

MICA and MICB act in the activation of the immune system by modulating NK cells and CD8+ T cell activities. These proteins do not form part of a complex but operate independently to signal the presence of infected or transformed cells to the immune system. They often appear on the cell surface under stress such as during viral infections or cancerous transformations activating immune cells to target and destroy these abnormal cells.

Pathways

The MICA/MICB interaction with NKG2D is integral to the immune checkpoint pathway. This pathway plays a significant role in regulating the immune system’s response to aberrant cells. Additionally MICA and MICB activity is linked to the regulation of the cytokine IFN-γ promoting the recruitment and activation of immune effector cells. The roles of these proteins in these pathways underline their importance in maintaining immune homeostasis and responding to pathological challenges.

MICA and MICB have notable associations with cancer and autoimmune diseases. Aberrant expression of these proteins on tumor cells facilitates their recognition and elimination by immune cells linking MICA and MICB directly to tumor immunosurveillance. In autoimmune disorders improper regulation of MICA/MICB expression can lead to undesirable immune cell activation contributing to conditions like rheumatoid arthritis. The proteins connect with others like NKG2D in both cancer immunity and autoimmune diseases highlighting their dual role in health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed : 10426993).

See full target information MICA

Additional targets

MICB

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:297 PubMed37274476

2023

Soluble NKG2D ligands impair CD8 T cell antitumor function dependent of NKG2D downregulation in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Feifei Luo,Kuiran Dong

Oncotarget 8:65743-65758 PubMed29029468

2017

Resveratrol promotes MICA/B expression and natural killer cell lysis of breast cancer cells by suppressing c-Myc/miR-17 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Pan,Jiaying Shen,Wengong Si,Chengyong Du,Danni Chen,Liang Xu,Minya Yao,Peifen Fu,Weimin Fan

Cancer biology & therapy 17:1253-1265 PubMed27834580

2016

NKG2D ligand expression in pediatric brain tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen Haberthur,Kathryn Brennan,Virginia Hoglund,Stephanie Balcaitis,Harrison Chinn,Amira Davis,Shannon Kreuser,Conrad Winter,Sarah E S Leary,Gail H Deutsch,Richard G Ellenbogen,Courtney A Crane

BMC cancer 12:24 PubMed22257486

2012

NKG2D ligand tumor expression and association with clinical outcome in early breast cancer patients: an observational study.

Applications

Unspecified application

Species

Unspecified reactive species

Esther M de Kruijf,Anita Sajet,Johanna G H van Nes,Hein Putter,Vincent T H B M Smit,Robert A Eagle,Insiya Jafferji,John Trowsdale,Gerrit Jan Liefers,Cornelis J H van de Velde,Peter J K Kuppen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MICA + MICB antibody [6D4]

Flow Cytometry - Anti-MICA + MICB antibody [6D4] (AB54413)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (4)

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Product promise