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AB203679

Anti-MICA + MICB antibody

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(5 Publications)

Rabbit Polyclonal MICB antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MICB conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PERB11.2, MICB, MHC class I polypeptide-related sequence B, MIC-B, PERB11.1, MICA, MHC class I polypeptide-related sequence A, MIC-A

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody (AB203679)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody (AB203679)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human lung carcinoma tissue labeling MICA + MICB with ab203679 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human MICB conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q29980

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Alternative Products

Primary Antibodies

AB224702

Anti-MICA + MICB antibody [EPR22031]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICA + MICB antibody [EPR22031] (AB224702)

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB302907

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Primary Antibodies

AB52587

Anti-PD1 antibody [NAT105]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>(or 1/50 - 1/200 if using a fluorescent secondary antibody)</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MICA and MICB also known as MIC-A and MIC-B are stress-induced proteins with significant roles in immune responses. These proteins are widely known for their presence on the surface of many human cell types most notably epithelial and endothelial cells. MICA is a glycoprotein with a molecular mass of approximately 70 kDa while MICB has a similar structure and mass. They are both part of the MHC class I-like molecules and function as ligands to the NKG2D receptor on natural killer (NK) cells and certain T cells a critical interaction for immune surveillance.
Biological function summary

MICA and MICB act in the activation of the immune system by modulating NK cells and CD8+ T cell activities. These proteins do not form part of a complex but operate independently to signal the presence of infected or transformed cells to the immune system. They often appear on the cell surface under stress such as during viral infections or cancerous transformations activating immune cells to target and destroy these abnormal cells.

Pathways

The MICA/MICB interaction with NKG2D is integral to the immune checkpoint pathway. This pathway plays a significant role in regulating the immune system’s response to aberrant cells. Additionally MICA and MICB activity is linked to the regulation of the cytokine IFN-γ promoting the recruitment and activation of immune effector cells. The roles of these proteins in these pathways underline their importance in maintaining immune homeostasis and responding to pathological challenges.

MICA and MICB have notable associations with cancer and autoimmune diseases. Aberrant expression of these proteins on tumor cells facilitates their recognition and elimination by immune cells linking MICA and MICB directly to tumor immunosurveillance. In autoimmune disorders improper regulation of MICA/MICB expression can lead to undesirable immune cell activation contributing to conditions like rheumatoid arthritis. The proteins connect with others like NKG2D in both cancer immunity and autoimmune diseases highlighting their dual role in health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8+ alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production.
See full target information MICB

Additional targets

MICA
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancer research communications 5:476-495 PubMed40116579

2025

23ME-01473, an Fc Effector-Enhanced Anti-ULBP6/2/5 Antibody, Restores NK Cell-Mediated Antitumor Immunity through NKG2D and FcγRIIIa Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Joel S Benjamin,Abigail Jarret,Shashank Bharill,Pierre Fontanillas,Shruti Yadav,Debasish Sen,Dina Ayupova,Danielle Kellar,Susanne Tilk,Clifford Hom,Zahra Bahrami Dizicheh,I-Ling Chen,Anh N Diep,Shi Shi,Nives Ivic,Caroline Bonnans,Alex Owyang,Pranidhi Sood,Germaine Fuh,Maike Schmidt,Kimberline Y Gerrick,Patrick Koenig,Mauro Poggio

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2202519 PubMed36683155

2023

Metabolic Reprogramming of NK Cells by Black Phosphorus Quantum Dots Potentiates Cancer Immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Lizhen He,Jianfu Zhao,Hongjun Li,Bin Xie,Ligeng Xu,Guanning Huang,Ting Liu,Zhen Gu,Tianfeng Chen

Journal of clinical and translational hepatology 11:614-625 PubMed36969901

2023

Topoisomerase I Inhibition Radiosensitizing Hepatocellular Carcinoma by RNF144A-mediated DNA-PKcs Ubiquitination and Natural Killer Cell Cytotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Chiao-Ling Tsai,Po-Sheng Yang,Feng-Ming Hsu,Ann-Lii Cheng,Wan-Ni Yu,Jason Chia-Hsien Cheng

Communications biology 5:1233 PubMed36371589

2022

Single-cell transcriptome analysis reveals cellular heterogeneity in mouse intra- and extra articular ligaments.

Applications

Unspecified application

Species

Unspecified reactive species

Kyota Ishibashi,Kentaro Ikegami,Takashi Shimbo,Eiji Sasaki,Tomomi Kitayama,Yuzuru Nakamura,Takahiro Tsushima,Yasuyuki Ishibashi,Katsuto Tamai

International journal of molecular sciences 22: PubMed34502547

2021

NK Cells Lose Their Cytotoxicity Function against Cancer Stem Cell-Rich Radiotherapy-Resistant Breast Cancer Cell Populations.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Jin,Hye Jung Kim
View all publications
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Product promise

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