AB150355

Anti-MICA + MICB antibody [EPR6568]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal MICA antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

PERB11.1, MICA, MHC class I polypeptide-related sequence A, MIC-A, PERB11.2, MICB, MHC class I polypeptide-related sequence B, MIC-B

4 Images

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Western blot - Anti-MICA + MICB antibody [EPR6568] (AB150355)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MICA + MICB antibody [EPR6568] (ab150355) at 1/1000 dilution

All lanes:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

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Unknown

Western blot - Anti-MICA + MICB antibody [EPR6568] (AB150355)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MICA + MICB antibody [EPR6568] (ab150355) at 1/1000 dilution

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

false

Unknown

Western blot - Anti-MICA + MICB antibody [EPR6568] (AB150355)

All lanes:

Western blot - Anti-MICA + MICB antibody [EPR6568] (ab150355) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 43 kDa

Observed band size: 43-70 kDa

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Supplier Data

Western blot - Anti-MICA + MICB antibody [EPR6568] (AB150355)

Exposure : Lane 1 : 3 seconds; Lane 2 : 10 seconds.

All lanes:

Western blot - Anti-MICA + MICB antibody [EPR6568] (ab150355) at 1/2000 dilution

Lane 1:

His-tagged human MICA recombinant protein, aa24-308 at 0.001 µg

Lane 2:

His and Fc tagged human MICB recombinant protein, aa1-298 at 0.001 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 60 kDa,100 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6568

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MICA and MICB also known as MIC-A and MIC-B are stress-induced proteins with significant roles in immune responses. These proteins are widely known for their presence on the surface of many human cell types most notably epithelial and endothelial cells. MICA is a glycoprotein with a molecular mass of approximately 70 kDa while MICB has a similar structure and mass. They are both part of the MHC class I-like molecules and function as ligands to the NKG2D receptor on natural killer (NK) cells and certain T cells a critical interaction for immune surveillance.

Biological function summary

MICA and MICB act in the activation of the immune system by modulating NK cells and CD8+ T cell activities. These proteins do not form part of a complex but operate independently to signal the presence of infected or transformed cells to the immune system. They often appear on the cell surface under stress such as during viral infections or cancerous transformations activating immune cells to target and destroy these abnormal cells.

Pathways

The MICA/MICB interaction with NKG2D is integral to the immune checkpoint pathway. This pathway plays a significant role in regulating the immune system’s response to aberrant cells. Additionally MICA and MICB activity is linked to the regulation of the cytokine IFN-γ promoting the recruitment and activation of immune effector cells. The roles of these proteins in these pathways underline their importance in maintaining immune homeostasis and responding to pathological challenges.

MICA and MICB have notable associations with cancer and autoimmune diseases. Aberrant expression of these proteins on tumor cells facilitates their recognition and elimination by immune cells linking MICA and MICB directly to tumor immunosurveillance. In autoimmune disorders improper regulation of MICA/MICB expression can lead to undesirable immune cell activation contributing to conditions like rheumatoid arthritis. The proteins connect with others like NKG2D in both cancer immunity and autoimmune diseases highlighting their dual role in health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed : 10426993).

See full target information MICA

Additional targets

MICB

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 222: PubMed37389656

2023

CLPTM1L is a GPI-anchoring pathway component targeted by HCMV.

Applications

Unspecified application

Species

Unspecified reactive species

Inbal Kol,Ahmed Rishiq,Mevaseret Cohen,Shira Kahlon,Ophir Pick,Liat Dassa,Natan Stein,Yotam Bar-On,Dana G Wolf,Einat Seidel,Ofer Mandelboim

Journal for immunotherapy of cancer 11: PubMed36754452

2023

MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihiro Morimoto,Nami Yamashita,Tatsuaki Daimon,Haruka Hirose,Shizuka Yamano,Naoki Haratake,Satoshi Ishikawa,Atrayee Bhattacharya,Atsushi Fushimi,Rehan Ahmad,Hidekazu Takahashi,Olga Dashevsky,Constantine Mitsiades,Donald Kufe

Nature communications 12:2061 PubMed33824318

2021

A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain.

Applications

Unspecified application

Species

Unspecified reactive species

Einat Seidel,Liat Dassa,Shira Kahlon,Boaz Tirosh,Anne Halenius,Tal Seidel Malkinson,Ofer Mandelboim

Journal of virology 89:7932-43 PubMed25995251

2015

Varicella-Zoster Virus and Herpes Simplex Virus 1 Differentially Modulate NKG2D Ligand Expression during Productive Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Tessa M Campbell,Brian P McSharry,Megan Steain,Barry Slobedman,Allison Abendroth

Cell reports 10:968-982 PubMed25683719

2015

Dynamic Co-evolution of Host and Pathogen: HCMV Downregulates the Prevalent Allele MICA∗008 to Escape Elimination by NK Cells.

Applications

Species

Human

Einat Seidel,Vu Thuy Khanh Le,Yotam Bar-On,Pinchas Tsukerman,Jonatan Enk,Rachel Yamin,Natan Stein,Dominik Schmiedel,Esther Oiknine Djian,Yiska Weisblum,Boaz Tirosh,Peter Stastny,Dana G Wolf,Hartmut Hengel,Ofer Mandelboim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MICA + MICB antibody [EPR6568]

Western blot - Anti-MICA + MICB antibody [EPR6568] (AB150355)

Western blot - Anti-MICA + MICB antibody [EPR6568] (AB150355)

Western blot - Anti-MICA + MICB antibody [EPR6568] (AB150355)

Western blot - Anti-MICA + MICB antibody [EPR6568] (AB150355)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (5)

Product promise