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AB220648

Anti-MICALL1 antibody

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(4 Publications)

Rabbit Polyclonal MICALL1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MICALL1 aa 750 to C-terminus.

View Alternative Names

KIAA1668, MIRAB13, MICALL1, MICAL-like protein 1, Molecule interacting with Rab13, MIRab13

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-MICALL1 antibody (AB220648)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MICALL1 antibody (AB220648)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A431 cells labeling MICALL1 with ab220648 at 4 μg/ml (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MICALL1 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8N3F8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MICALL1 also known as MICAL-like 1 is a protein that plays a significant role in cellular trafficking and organization. MICALL1 facilitates the regulation of endocytic recycling. The protein contains several functional domains contributing to its activities within the cell and it has an approximate mass of 65 kDa. This protein expresses widely in human tissues including the brain and heart. MICALL1 exerts its functions mainly at the plasma membrane and in endosomal compartments where it influences the sorting and recycling of proteins.
Biological function summary

MICALL1 interacts with other proteins to modulate membrane dynamics. It forms part of a larger protein complex that includes small GTPases such as Rab proteins which are essential for vesicular fusion processes. MICALL1 binds to actin filaments and serves as a scaffold facilitating the reorganization of the cytoskeleton during membrane trafficking. This protein contributes to cellular processes including migration adhesion and synaptic vesicle recycling highlighting its importance in maintaining cellular homeostasis.

Pathways

MICALL1 functions in the coordination of the endocytic recycling pathway and may interact with proteins related to the Arp2/3 complex. It participates in pathways alongside EHD family proteins such as EHD1 that help maintain efficient trafficking between the plasma membrane and endosomes. MICALL1's actions ensure proper sorting and recycling of cargoes ensuring cellular responsiveness and adaptability to environmental cues.

MICALL1 has associations with certain neurodegenerative diseases and cancer. Impaired functioning of MICALL1 disrupts cellular trafficking processes contributing to the pathogenesis of Alzheimer's disease due to its impact on synaptic function. Altered MICALL1 expression correlates with some types of cancer possibly due to its involvement in cell migration and invasion. In these conditions interactions with other proteins like EHD1 might influence disease progression and cellular behavior.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes. On endosome membranes, may act as a downstream effector of Rab proteins recruiting cytosolic proteins to regulate membrane tubulation. May be involved in a late step of receptor-mediated endocytosis regulating for instance endocytosed-EGF receptor trafficking. Alternatively, may regulate slow endocytic recycling of endocytosed proteins back to the plasma membrane. May indirectly play a role in neurite outgrowth.
See full target information MICALL1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 35:ar144 PubMed39382837

2024

Endosomal actin branching, fission, and receptor recycling require FCHSD2 recruitment by MICAL-L1.

Applications

Unspecified application

Species

Unspecified reactive species

Devin Frisby,Ajay B Murakonda,Bazella Ashraf,Kanika Dhawan,Leonardo Almeida-Souza,Naava Naslavsky,Steve Caplan

Cellular and molecular life sciences : CMLS 79:615 PubMed36460928

2022

The endocytic recycling compartment serves as a viral factory for hepatitis E virus.

Applications

Unspecified application

Species

Unspecified reactive species

Cyrine Bentaleb,Kévin Hervouet,Claire Montpellier,Charline Camuzet,Martin Ferrié,Julien Burlaud-Gaillard,Stéphane Bressanelli,Karoline Metzger,Elisabeth Werkmeister,Maliki Ankavay,Nancy Leon Janampa,Julien Marlet,Julien Roux,Clarence Deffaud,Anne Goffard,Yves Rouillé,Jean Dubuisson,Philippe Roingeard,Cécile-Marie Aliouat-Denis,Laurence Cocquerel

The Journal of biological chemistry 296:100190 PubMed33334886

2021

Defining the protein and lipid constituents of tubular recycling endosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Trey Farmer,Shuwei Xie,Naava Naslavsky,Jacqueline Stöckli,David E James,Steve Caplan

Scientific reports 9:19619 PubMed31873114

2019

miR-192-5p suppresses the progression of lung cancer bone metastasis by targeting TRIM44.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zou,Menghai Zhu,Chong Lian,Jiaqiang Wang,Zhiquan Chen,Xiaoming Zhang,Yongchao Yang,Xinfeng Chen,Xinhui Cui,Jijun Liu,Hexuan Wang,Qi Wen,Ji Yi
View all publications
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