AB190114

Anti-MICU1 antibody

Name: Anti-MICU1 antibody (ab190114) | Abcam
Brand: Abcam Limited
SKU: AB190114
Rating: 4 (4 reviews)

(4 Reviews)

(7 Publications)

Goat Polyclonal MICU1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MICU1 aa 100-150.

View Alternative Names

CALC, CBARA1, MICU1, Atopy-related autoantigen CALC, Calcium-binding atopy-related autoantigen 1, ara CALC

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB190114)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB190114)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung, respiratory epithelium labeling MICU1 with ab190114 at 3.75 μg/ml.

Supplier Data

Western blot - Anti-MICU1 antibody (AB190114)

Primary incubation was 1 hour.

An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.

All lanes:

Western blot - Anti-MICU1 antibody (ab190114) at 0.5 µg/mL

All lanes:

K562 lysate (in RIPA buffer) at 35 µg

Predicted band size: 54 kDa

Observed band size: 55 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MICU1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q9BPX6

Specificity

ab190114 is expected to recognize reported isoforms 1 and 2 (NP_006068.2; NP_001182447.1).

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MICU1 also known as "Mitochondrial Calcium Uptake 1" is a regulator of mitochondrial calcium uniporter (MCU) activity. It has a molecular mass of approximately 54 kDa. MICU1 resides mainly in the mitochondria where it is involved in controlling calcium ion flow across the mitochondrial membranes. This calcium regulation is important for cellular energy production and metabolism. The expression of MICU1 is seen in various tissues with significant presence in those requiring high energy such as the heart brain and skeletal muscles.

Biological function summary

By regulating calcium uptake into mitochondria MICU1 protects cells from calcium overload and mitochondrial damage. This protein is part of the mitochondrial calcium uniporter complex which includes other proteins such as MCU MICU2 and EMRE. The complex functions to maintain calcium homeostasis playing a role in cellular metabolism and ATP production. The balanced uptake of calcium by MICU1-mediated mechanisms supports proper mitochondrial function and reactive oxygen species handling.

Pathways

The role of MICU1 is significant in calcium signaling and metabolic pathways. It interacts with the mitochondrial calcium uniporter complex (MCUC) to regulate calcium influx impacting cellular metabolism and energy production. Through these pathways MICU1 relates closely to other proteins such as MICU2 and MCU ensuring that cells respond to environmental and metabolic cues. MICU1's modulation of calcium levels also ties it to pathways involved in apoptosis and cell survival.

MICU1 disruptions associate with neuromuscular diseases and metabolic disorders. Altered MICU1 function can lead to issues such as muscular dystrophy and cognitive impairments due to ineffective calcium regulation in mitochondria. The interplay of MICU1 with MCU can affect cellular energy status and contribute to the pathology of these diseases. Understanding MICU1 and its pathway interactions is essential for exploring therapeutic strategies for related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains (PubMed : 20693986, PubMed : 23101630, PubMed : 23747253, PubMed : 24313810, PubMed : 24332854, PubMed : 24503055, PubMed : 24560927, PubMed : 26341627, PubMed : 26903221, PubMed : 27099988, PubMed : 28615291, PubMed : 30454562, PubMed : 30638448, PubMed : 32494073, PubMed : 32667285, PubMed : 32762847, PubMed : 32790952, PubMed : 34463251, PubMed : 36206740, PubMed : 37036971, PubMed : 37126688). MICU1 and MICU2 (or MICU3) form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium (PubMed : 24560927, PubMed : 26903221, PubMed : 28615291, PubMed : 32148862, PubMed : 32494073, PubMed : 32667285, PubMed : 32762847, PubMed : 32790952, PubMed : 36206740, PubMed : 37036971, PubMed : 37126688). At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (PubMed : 32494073, PubMed : 32667285, PubMed : 32762847, PubMed : 37036971, PubMed : 37126688). At higher calcium levels, calcium-binding to MICU1 and MICU2 (or MICU3) induces a conformational change that weakens MCU-MICU1 interactions and moves the MICU1-MICU2 heterodimer away from the pore, allowing calcium permeation through the MCU channel (PubMed : 32494073, PubMed : 32667285, PubMed : 32762847). Also required to protect against manganese toxicity by preventing manganese uptake by MCU : mechanistically, manganese-binding to its EF-hand domains does not induce any conformational change, maintaining MCU pore occlusion (PubMed : 30082385, PubMed : 30403999). Also acts as a barrier for inhibitors of the MCU channel, such as ruthenium red or its derivative Ru360 (PubMed : 37244260). Acts as a regulator of mitochondrial cristae structure independently of its ability to regulate the mitochondrial calcium uniporter channel (PubMed : 31427612, PubMed : 37098122). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed : 22904319). Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG (PubMed : 16002733).. Isoform 6. Isoform that regulates mitochondrial calcium uniporter (MCU) in the skeletal muscle (By similarity). Compared to other isoforms, this isoform has higher affinity for calcium, promoting mitochondrial calcium uptake at lower calcium concentrations (By similarity). This allows a rapid response of mitochondrial metabolism and ensures sustained ATP production needed for resistance and strenuous exercise (By similarity).

See full target information MICU1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 7:714-729 PubMed40200126

2025

TMEM65 regulates and is required for NCLX-dependent mitochondrial calcium efflux.

Applications

Unspecified application

Species

Unspecified reactive species

Joanne F Garbincius,Oniel Salik,Henry M Cohen,Carmen Choya-Foces,Adam S Mangold,Angelina D Makhoul,Anna E Schmidt,Dima Y Khalil,Joshua J Doolittle,Anya S Wilkinson,Emma K Murray,Michael P Lazaropoulos,Alycia N Hildebrand,Dhanendra Tomar,John W Elrod

Shock (Augusta, Ga.) 60:698-706 PubMed37695737

2023

SEPSIS LEADS TO IMPAIRED MITOCHONDRIAL CALCIUM UPTAKE AND SKELETAL MUSCLE WEAKNESS BY REDUCING THE MICU1:MCU PROTEIN RATIO.

Applications

Unspecified application

Species

Unspecified reactive species

Xuexin Li,Bowen Sun,Jie Li,Wanlin Ye,Mingjuan Li,Fasheng Guan,Songlin Wu,Xuerong Luo,Jianguo Feng,Jing Jia,Xueru Liu,Tao Li,Li Liu

International journal of oncology 63: PubMed37264968

2023

Targeting the mitochondrial calcium uniporter inhibits cancer progression and alleviates cisplatin resistance in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Miao,Xiaofei Wang,Yafang Lai,Ying Huang,Hua Yin,Xiangkun Meng,Hao Liu,Ruirui Hou,Wan Lin,Xiaoxu Zhang,Xu Zhang,Bei Cho Chai,Feixiong Zhang,Le Guo,Shaoqi Yang

Genome biology 23:193 PubMed36096941

2022

Control of immediate early gene expression by CPEB4-repressor complex-mediated mRNA degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Poetz,Svetlana Lebedeva,Johanna Schott,Doris Lindner,Uwe Ohler,Georg Stoecklin

Bioengineered 13:227-241 PubMed34847839

2021

Dihydroartemisinin represses oral squamous cell carcinoma progression through downregulating mitochondrial calcium uniporter.

Applications

Unspecified application

Species

Unspecified reactive species

Shen Zheng,Ran Wu,Yunlong Deng,Qiang Zhang

BioMed research international 2021:6650791 PubMed34189138

2021

MCU That Is Transcriptionally Regulated by Nrf2 Augments Malignant Biological Behaviors in Oral Squamous Cell Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Wu,Weiwen Zuo,Xiaoliang Xu,Lei Bi,Chunguang Zhang,Hui Chen,Hui Liu

Frontiers in oncology 10:516746 PubMed33425712

2020

Inhibition of Cathepsin S Induces Mitochondrial Apoptosis in Glioblastoma Cell Lines Through Mitochondrial Stress and Autophagosome Accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Maoxing Fei,Li Zhang,Handong Wang,Yihao Zhu,Wenhao Niu,Ting Tang,Yanling Han

View all publications

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Anti-MICU1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB190114)

Western blot - Anti-MICU1 antibody (AB190114)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise