Goat Polyclonal MICU1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MICU1 aa 100-150.
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Publications
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- International journal of oncology 63:2023Targeting the mitochondrial calcium uniporter inhibits cancer progression and alleviates cisplatin resistance in esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Miao,Xiaofei Wang,Yafang Lai,Ying Huang,Hua Yin,Xiangkun Meng,Hao Liu,Ruirui Hou,Wan Lin,Xiaoxu Zhang,Xu Zhang,Bei Cho Chai,Feixiong Zhang,Le Guo,Shaoqi YangPubMed 37264968
- BioMed research international 2021:66507912021MCU That Is Transcriptionally Regulated by Nrf2 Augments Malignant Biological Behaviors in Oral Squamous Cell Carcinoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRan Wu,Weiwen Zuo,Xiaoliang Xu,Lei Bi,Chunguang Zhang,Hui Chen,Hui LiuPubMed 34189138
- Frontiers in oncology 10:5167462020Inhibition of Cathepsin S Induces Mitochondrial Apoptosis in Glioblastoma Cell Lines Through Mitochondrial Stress and Autophagosome Accumulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaoxing Fei et. al.PubMed 33425712
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MICU1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BPX6
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Goat | Predicted | Predicted |
| Hamster | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Goat, Horse, Chicken, Hamster, Cow, Dog, Pig, Zebrafish, Cynomolgus monkey, Xenopus tropicalis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.75 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Goat, Horse, Chicken, Hamster, Cow, Dog, Pig, Zebrafish, Cynomolgus monkey, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
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Target data
Function
Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains (PubMed:20693986, PubMed:23101630, PubMed:23747253, PubMed:24313810, PubMed:24332854, PubMed:24503055, PubMed:24560927, PubMed:26341627, PubMed:26903221, PubMed:27099988, PubMed:28615291, PubMed:30454562, PubMed:30638448, PubMed:32494073, PubMed:32667285, PubMed:32762847, PubMed:32790952, PubMed:34463251, PubMed:36206740, PubMed:37036971, PubMed:37126688). MICU1 and MICU2 (or MICU3) form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium (PubMed:24560927, PubMed:26903221, PubMed:28615291, PubMed:32148862, PubMed:32494073, PubMed:32667285, PubMed:32762847, PubMed:32790952, PubMed:36206740, PubMed:37036971, PubMed:37126688). At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (PubMed:32494073, PubMed:32667285, PubMed:32762847, PubMed:37036971, PubMed:37126688). At higher calcium levels, calcium-binding to MICU1 and MICU2 (or MICU3) induces a conformational change that weakens MCU-MICU1 interactions and moves the MICU1-MICU2 heterodimer away from the pore, allowing calcium permeation through the MCU channel (PubMed:32494073, PubMed:32667285, PubMed:32762847). Also required to protect against manganese toxicity by preventing manganese uptake by MCU: mechanistically, manganese-binding to its EF-hand domains does not induce any conformational change, maintaining MCU pore occlusion (PubMed:30082385, PubMed:30403999). Also acts as a barrier for inhibitors of the MCU channel, such as ruthenium red or its derivative Ru360 (PubMed:37244260). Acts as a regulator of mitochondrial cristae structure independently of its ability to regulate the mitochondrial calcium uniporter channel (PubMed:31427612, PubMed:37098122). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed:22904319). Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG (PubMed:16002733). Isoform 6. Isoform that regulates mitochondrial calcium uniporter (MCU) in the skeletal muscle (By similarity). Compared to other isoforms, this isoform has higher affinity for calcium, promoting mitochondrial calcium uptake at lower calcium concentrations (By similarity). This allows a rapid response of mitochondrial metabolism and ensures sustained ATP production needed for resistance and strenuous exercise (By similarity).
Alternative names
CALC, CBARA1, MICU1, Atopy-related autoantigen CALC, Calcium-binding atopy-related autoantigen 1, ara CALC
Recommended products
Goat Polyclonal MICU1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MICU1 aa 100-150.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MICU1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BPX6
- Purification technique
- Affinity purification Immunogen
- Specificity
ab190114 is expected to recognize reported isoforms 1 and 2 (NP_006068.2; NP_001182447.1).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MICU1 also known as "Mitochondrial Calcium Uptake 1" is a regulator of mitochondrial calcium uniporter (MCU) activity. It has a molecular mass of approximately 54 kDa. MICU1 resides mainly in the mitochondria where it is involved in controlling calcium ion flow across the mitochondrial membranes. This calcium regulation is important for cellular energy production and metabolism. The expression of MICU1 is seen in various tissues with significant presence in those requiring high energy such as the heart brain and skeletal muscles.
- Biological function summary
By regulating calcium uptake into mitochondria MICU1 protects cells from calcium overload and mitochondrial damage. This protein is part of the mitochondrial calcium uniporter complex which includes other proteins such as MCU MICU2 and EMRE. The complex functions to maintain calcium homeostasis playing a role in cellular metabolism and ATP production. The balanced uptake of calcium by MICU1-mediated mechanisms supports proper mitochondrial function and reactive oxygen species handling.
- Pathways
The role of MICU1 is significant in calcium signaling and metabolic pathways. It interacts with the mitochondrial calcium uniporter complex (MCUC) to regulate calcium influx impacting cellular metabolism and energy production. Through these pathways MICU1 relates closely to other proteins such as MICU2 and MCU ensuring that cells respond to environmental and metabolic cues. MICU1's modulation of calcium levels also ties it to pathways involved in apoptosis and cell survival.
- Associated diseases and disorders
MICU1 disruptions associate with neuromuscular diseases and metabolic disorders. Altered MICU1 function can lead to issues such as muscular dystrophy and cognitive impairments due to ineffective calcium regulation in mitochondria. The interplay of MICU1 with MCU can affect cellular energy status and contribute to the pathology of these diseases. Understanding MICU1 and its pathway interactions is essential for exploring therapeutic strategies for related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-MICU1 antibody (ab190114)
Primary incubation was 1 hour.
An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.
All lanes: Western blot - Anti-MICU1 antibody (ab190114) at 0.5 µg/mL
All lanes: K562 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (ab190114)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung, respiratory epithelium labeling MICU1 with ab190114 at 3.75 μg/ml.
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