Rabbit Polyclonal MICU1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MICU1 aa 250-400.
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- Stem cell research & therapy 12:2692021PINK1 contained in huMSC-derived exosomes prevents cardiomyocyte mitochondrial calcium overload in sepsis via recovery of mitochondrial Ca efflux.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Zhou,Min Xie,Jing Zhu,Qin Yi,Bin Tan,Yasha Li,Liang Ye,Xinyuan Zhang,Ying Zhang,Jie Tian,Hao XuPubMed 33957982
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MICU1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BPX6
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Giant Panda | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Cynomolgus monkey, Giant Panda | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Cynomolgus monkey, Giant Panda | Dilution info - | Notes - |
Associated Products
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Target data
Function
Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains (PubMed:20693986, PubMed:23101630, PubMed:23747253, PubMed:24313810, PubMed:24332854, PubMed:24503055, PubMed:24560927, PubMed:26341627, PubMed:26903221, PubMed:27099988, PubMed:28615291, PubMed:30454562, PubMed:30638448, PubMed:32494073, PubMed:32667285, PubMed:32762847, PubMed:32790952, PubMed:34463251, PubMed:36206740, PubMed:37036971, PubMed:37126688). MICU1 and MICU2 (or MICU3) form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium (PubMed:24560927, PubMed:26903221, PubMed:28615291, PubMed:32148862, PubMed:32494073, PubMed:32667285, PubMed:32762847, PubMed:32790952, PubMed:36206740, PubMed:37036971, PubMed:37126688). At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (PubMed:32494073, PubMed:32667285, PubMed:32762847, PubMed:37036971, PubMed:37126688). At higher calcium levels, calcium-binding to MICU1 and MICU2 (or MICU3) induces a conformational change that weakens MCU-MICU1 interactions and moves the MICU1-MICU2 heterodimer away from the pore, allowing calcium permeation through the MCU channel (PubMed:32494073, PubMed:32667285, PubMed:32762847). Also required to protect against manganese toxicity by preventing manganese uptake by MCU: mechanistically, manganese-binding to its EF-hand domains does not induce any conformational change, maintaining MCU pore occlusion (PubMed:30082385, PubMed:30403999). Also acts as a barrier for inhibitors of the MCU channel, such as ruthenium red or its derivative Ru360 (PubMed:37244260). Acts as a regulator of mitochondrial cristae structure independently of its ability to regulate the mitochondrial calcium uniporter channel (PubMed:31427612, PubMed:37098122). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed:22904319). Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG (PubMed:16002733). Isoform 6. Isoform that regulates mitochondrial calcium uniporter (MCU) in the skeletal muscle (By similarity). Compared to other isoforms, this isoform has higher affinity for calcium, promoting mitochondrial calcium uptake at lower calcium concentrations (By similarity). This allows a rapid response of mitochondrial metabolism and ensures sustained ATP production needed for resistance and strenuous exercise (By similarity).
Alternative names
CALC, CBARA1, MICU1, Atopy-related autoantigen CALC, Calcium-binding atopy-related autoantigen 1, ara CALC
Recommended products
Rabbit Polyclonal MICU1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MICU1 aa 250-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MICU1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BPX6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MICU1 also known as "Mitochondrial Calcium Uptake 1" is a regulator of mitochondrial calcium uniporter (MCU) activity. It has a molecular mass of approximately 54 kDa. MICU1 resides mainly in the mitochondria where it is involved in controlling calcium ion flow across the mitochondrial membranes. This calcium regulation is important for cellular energy production and metabolism. The expression of MICU1 is seen in various tissues with significant presence in those requiring high energy such as the heart brain and skeletal muscles.
- Biological function summary
By regulating calcium uptake into mitochondria MICU1 protects cells from calcium overload and mitochondrial damage. This protein is part of the mitochondrial calcium uniporter complex which includes other proteins such as MCU MICU2 and EMRE. The complex functions to maintain calcium homeostasis playing a role in cellular metabolism and ATP production. The balanced uptake of calcium by MICU1-mediated mechanisms supports proper mitochondrial function and reactive oxygen species handling.
- Pathways
The role of MICU1 is significant in calcium signaling and metabolic pathways. It interacts with the mitochondrial calcium uniporter complex (MCUC) to regulate calcium influx impacting cellular metabolism and energy production. Through these pathways MICU1 relates closely to other proteins such as MICU2 and MCU ensuring that cells respond to environmental and metabolic cues. MICU1's modulation of calcium levels also ties it to pathways involved in apoptosis and cell survival.
- Associated diseases and disorders
MICU1 disruptions associate with neuromuscular diseases and metabolic disorders. Altered MICU1 function can lead to issues such as muscular dystrophy and cognitive impairments due to ineffective calcium regulation in mitochondria. The interplay of MICU1 with MCU can affect cellular energy status and contribute to the pathology of these diseases. Understanding MICU1 and its pathway interactions is essential for exploring therapeutic strategies for related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-MICU1 antibody (ab224161)
All lanes: Western blot - Anti-MICU1 antibody (ab224161) at 0.4 µg/mL
All lanes: A549 (human lung carcinoma cell line) whole cell lysate
Predicted band size: 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (ab224161)
Immunohistochemical analysis of human kidney tissue labeling MICU1 in the cytoplasm of cells in tubules with ab224161 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (ab224161)
Immunohistochemical analysis of human endometrium tissue labeling MICU1 in the cytoplasm of glandular cells with ab224161 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (ab224161)
Immunohistochemical analysis of human liver tissue labeling MICU1 in the cytoplasm of hepatocytes with ab224161 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (ab224161)
Immunohistochemical analysis of human rectum tissue labeling MICU1 in the cytoplasm of glandular cells with ab224161 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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