AB224161

Anti-MICU1 antibody

Name: Anti-MICU1 antibody (ab224161) | Abcam
Brand: Abcam Limited
SKU: AB224161
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal MICU1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MICU1 aa 250-400.

View Alternative Names

CALC, CBARA1, MICU1, Atopy-related autoantigen CALC, Calcium-binding atopy-related autoantigen 1, ara CALC

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB224161)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB224161)

Immunohistochemical analysis of human liver tissue labeling MICU1 in the cytoplasm of hepatocytes with ab224161 at 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB224161)

Immunohistochemical analysis of human kidney tissue labeling MICU1 in the cytoplasm of cells in tubules with ab224161 at 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB224161)

Immunohistochemical analysis of human endometrium tissue labeling MICU1 in the cytoplasm of glandular cells with ab224161 at 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB224161)

Immunohistochemical analysis of human rectum tissue labeling MICU1 in the cytoplasm of glandular cells with ab224161 at 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MICU1 antibody (AB224161)

All lanes:

Western blot - Anti-MICU1 antibody (ab224161) at 0.4 µg/mL

All lanes:

A549 (human lung carcinoma cell line) whole cell lysate

Predicted band size: 54 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MICU1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q9BPX6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Giant Panda": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MICU1 also known as "Mitochondrial Calcium Uptake 1" is a regulator of mitochondrial calcium uniporter (MCU) activity. It has a molecular mass of approximately 54 kDa. MICU1 resides mainly in the mitochondria where it is involved in controlling calcium ion flow across the mitochondrial membranes. This calcium regulation is important for cellular energy production and metabolism. The expression of MICU1 is seen in various tissues with significant presence in those requiring high energy such as the heart brain and skeletal muscles.

Biological function summary

By regulating calcium uptake into mitochondria MICU1 protects cells from calcium overload and mitochondrial damage. This protein is part of the mitochondrial calcium uniporter complex which includes other proteins such as MCU MICU2 and EMRE. The complex functions to maintain calcium homeostasis playing a role in cellular metabolism and ATP production. The balanced uptake of calcium by MICU1-mediated mechanisms supports proper mitochondrial function and reactive oxygen species handling.

Pathways

The role of MICU1 is significant in calcium signaling and metabolic pathways. It interacts with the mitochondrial calcium uniporter complex (MCUC) to regulate calcium influx impacting cellular metabolism and energy production. Through these pathways MICU1 relates closely to other proteins such as MICU2 and MCU ensuring that cells respond to environmental and metabolic cues. MICU1's modulation of calcium levels also ties it to pathways involved in apoptosis and cell survival.

MICU1 disruptions associate with neuromuscular diseases and metabolic disorders. Altered MICU1 function can lead to issues such as muscular dystrophy and cognitive impairments due to ineffective calcium regulation in mitochondria. The interplay of MICU1 with MCU can affect cellular energy status and contribute to the pathology of these diseases. Understanding MICU1 and its pathway interactions is essential for exploring therapeutic strategies for related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains (PubMed : 20693986, PubMed : 23101630, PubMed : 23747253, PubMed : 24313810, PubMed : 24332854, PubMed : 24503055, PubMed : 24560927, PubMed : 26341627, PubMed : 26903221, PubMed : 27099988, PubMed : 28615291, PubMed : 30454562, PubMed : 30638448, PubMed : 32494073, PubMed : 32667285, PubMed : 32762847, PubMed : 32790952, PubMed : 34463251, PubMed : 36206740, PubMed : 37036971, PubMed : 37126688). MICU1 and MICU2 (or MICU3) form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium (PubMed : 24560927, PubMed : 26903221, PubMed : 28615291, PubMed : 32148862, PubMed : 32494073, PubMed : 32667285, PubMed : 32762847, PubMed : 32790952, PubMed : 36206740, PubMed : 37036971, PubMed : 37126688). At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (PubMed : 32494073, PubMed : 32667285, PubMed : 32762847, PubMed : 37036971, PubMed : 37126688). At higher calcium levels, calcium-binding to MICU1 and MICU2 (or MICU3) induces a conformational change that weakens MCU-MICU1 interactions and moves the MICU1-MICU2 heterodimer away from the pore, allowing calcium permeation through the MCU channel (PubMed : 32494073, PubMed : 32667285, PubMed : 32762847). Also required to protect against manganese toxicity by preventing manganese uptake by MCU : mechanistically, manganese-binding to its EF-hand domains does not induce any conformational change, maintaining MCU pore occlusion (PubMed : 30082385, PubMed : 30403999). Also acts as a barrier for inhibitors of the MCU channel, such as ruthenium red or its derivative Ru360 (PubMed : 37244260). Acts as a regulator of mitochondrial cristae structure independently of its ability to regulate the mitochondrial calcium uniporter channel (PubMed : 31427612, PubMed : 37098122). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed : 22904319). Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG (PubMed : 16002733).. Isoform 6. Isoform that regulates mitochondrial calcium uniporter (MCU) in the skeletal muscle (By similarity). Compared to other isoforms, this isoform has higher affinity for calcium, promoting mitochondrial calcium uptake at lower calcium concentrations (By similarity). This allows a rapid response of mitochondrial metabolism and ensures sustained ATP production needed for resistance and strenuous exercise (By similarity).

See full target information MICU1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 43:5972-6000 PubMed39448884

2024

Cyclophilin D plays a critical role in the survival of senescent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Margherita Protasoni,Vanessa López-Polo,Camille Stephan-Otto Attolini,Julian Brandariz,Nicolas Herranz,Joaquin Mateo,Sergio Ruiz,Oscar Fernandez-Capetillo,Marta Kovatcheva,Manuel Serrano

Stem cell research & therapy 12:269 PubMed33957982

2021

PINK1 contained in huMSC-derived exosomes prevents cardiomyocyte mitochondrial calcium overload in sepsis via recovery of mitochondrial Ca efflux.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Zhou,Min Xie,Jing Zhu,Qin Yi,Bin Tan,Yasha Li,Liang Ye,Xinyuan Zhang,Ying Zhang,Jie Tian,Hao Xu

International journal of oncology 57:1001-1012 PubMed32945386

2020

lncRNA NEAT1 regulates the proliferation and migration of hepatocellular carcinoma cells by acting as a miR‑320a molecular sponge and targeting L antigen family member 3.

Applications

Unspecified application

Species

Unspecified reactive species

Yixi Zhang,Changjun Huang,Zebin Zhu,Yufei Hou,Shanzhou Huang,Chengjun Sun,Yunhua Tang,Zhiheng Zhang,Linhe Wang,Huadi Chen,Weiqiang Ju,Xin Qiao,Maogen Chen

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Product promise

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Anti-MICU1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB224161)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB224161)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB224161)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB224161)

Western blot - Anti-MICU1 antibody (AB224161)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise