AB101465

Anti-MICU2 antibody

Name: Anti-MICU2 antibody. Rabbit polyclonal (ab101465) | Abcam
Brand: Abcam Limited
SKU: AB101465
Rating: 4 (3 reviews)

(3 Reviews)

(46 Publications)

Rabbit Polyclonal MICU2 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 46 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MICU2 aa 50-250.

View Alternative Names

EFHA1, MICU2, hMICU3, EF-hand domain-containing family member A1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU2 antibody (AB101465)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU2 antibody (AB101465)

Immunohistochemical analysis of FFPE human hepatoma tissue, labeling MICU2 with ab101465 (diluted 1/500).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU2 antibody (AB101465)

Immunohistochemical analysis of FFPE mouse kidney tissue, labeling MICU2 with ab101465 (diluted 1/500).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MICU2 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

Q8IYU8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MICU2 also known as mitochondrial calcium uptake 2 is an important regulator of calcium ion influx into mitochondria. It is a 54 kDa protein that resides primarily in the inner mitochondrial membrane where it helps maintain calcium homeostasis. By modulating calcium uptake MICU2 influences various cellular processes and mitochondrial dynamics. Its expression levels vary among the tissues with notable levels observed in muscle and brain tissues.

Biological function summary

MICU2 is a component of the mitochondrial calcium uniporter complex which includes other proteins such as MCU and MICU1. This complex ensures proper calcium ion transfer across the mitochondrial membrane which is essential for ATP production maintenance of mitochondrial membrane potential and regulation of apoptotic signaling. MICU2 works as a gatekeeper preventing calcium overload and facilitating controlled entry of calcium ions.

Pathways

MICU2 is involved in pathways essential for cellular energy metabolism and apoptosis. It participates in the calcium signaling pathway where it functions alongside MCU to control calcium entry impacting processes like oxidative phosphorylation. Another related pathway is the intrinsic apoptotic pathway where MICU2’s regulation of calcium levels can influence mitochondrial-mediated apoptosis. In this context MICU1 acts as a supportive partner by providing threshold control for calcium uptake.

MICU2 is linked to neurodegenerative diseases and mitochondrial dysfunction disorders. Abnormalities in MICU2 expression or function can lead to improper calcium homeostasis contributing to conditions like Alzheimer's disease by affecting neuronal calcium signaling. The interaction of MICU2 with MCU and MICU1 within the uniporter complex is critical for maintaining cellular health and disruptions in these relationships may exacerbate disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains (PubMed : 24503055, PubMed : 24560927, PubMed : 26903221, PubMed : 28615291, PubMed : 30699349, PubMed : 31397067, PubMed : 32494073, PubMed : 32667285, PubMed : 32762847, PubMed : 32790952). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium (PubMed : 24560927, PubMed : 26903221, PubMed : 28615291, PubMed : 30699349, PubMed : 31397067, PubMed : 32148862, PubMed : 32494073, PubMed : 32667285, PubMed : 32762847, PubMed : 32790952). At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (PubMed : 32494073, PubMed : 32667285, PubMed : 32762847). At higher calcium levels, calcium-binding to MICU1 and MICU2 induces a conformational change that weakens MCU-MICU1 interactions and moves the MICU1-MICU2 heterodimer away from the pore, allowing calcium permeation through the MCU channel (PubMed : 32494073, PubMed : 32667285, PubMed : 32762847).

See full target information MICU2

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Publications (46)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1348 PubMed40973741

2025

Interaction of the mitochondrial calcium/proton exchanger TMBIM5 with MICU1.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zhang,Benjamin Gottschalk,Felicia Dietsche,Sara Bitar,Diones Bueno,Liliana Rojas-Charry,Anshu Kumari,Vivek Garg,Wolfgang F Graier,Axel Methner

Nature communications 16:4029 PubMed40301431

2025

Mitochondrial membrane hyperpolarization modulates nuclear DNA methylation and gene expression through phospholipid remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Mateus Prates Mori,Oswaldo A Lozoya,Ashley M Brooks,Carl D Bortner,Cristina A Nadalutti,Birgitta Ryback,Brittany P Rickard,Marta Overchuk,Imran Rizvi,Tatiana Rogasevskaia,Kai Ting Huang,Prottoy Hasan,György Hajnóczky,Janine H Santos

Antioxidants (Basel, Switzerland) 14: PubMed40227270

2025

Oxidative Stress in Brain Function.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela-Marilena Trofin,Dragos-Petrica Sardaru,Dan Trofin,Ilie Onu,Andrei Tutu,Ana Onu,Cristiana Onită,Anca Irina Galaction,Daniela Viorelia Matei

Nature communications 16:2810 PubMed40118824

2025

Dependence of mitochondrial calcium signalling and dynamics on the disaggregase, CLPB.

Applications

Unspecified application

Species

Unspecified reactive species

Donato D'Angelo,Víctor H Sánchez-Vázquez,Benjamín Cartes-Saavedra,Denis Vecellio Reane,Ryan R Cupo,Hilda Delgado de la Herran,Giorgia Ghirardo,James Shorter,Ron A Wevers,Saskia B Wortmann,Fabiana Perocchi,Rosario Rizzuto,Anna Raffaello,György Hajnóczky

The EMBO journal 43:5972-6000 PubMed39448884

2024

Cyclophilin D plays a critical role in the survival of senescent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Margherita Protasoni,Vanessa López-Polo,Camille Stephan-Otto Attolini,Julian Brandariz,Nicolas Herranz,Joaquin Mateo,Sergio Ruiz,Oscar Fernandez-Capetillo,Marta Kovatcheva,Manuel Serrano

Cancer discovery 14:1922-1939 PubMed38787341

2024

Targeting Acute Myeloid Leukemia Stem Cells through Perturbation of Mitochondrial Calcium.

Applications

Unspecified application

Species

Unspecified reactive species

Anagha Inguva Sheth,Mark J Althoff,Hunter Tolison,Krysta Engel,Maria L Amaya,Anna E Krug,Tracy N Young,Mohammad Minhajuddin,Shanshan Pei,Sweta B Patel,Amanda Winters,Regan Miller,Ian T Shelton,Jonathan St-Germain,Tianyi Ling,Courtney L Jones,Brian Raught,Austin E Gillen,Monica Ransom,Sarah Staggs,Clayton A Smith,Daniel A Pollyea,Brett M Stevens,Craig T Jordan

Cell reports 42:113544 PubMed38060381

2023

A mitochondrial inside-out iron-calcium signal reveals drug targets for Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Vinita Bharat,Aarooran S Durairaj,Roeland Vanhauwaert,Li Li,Colin M Muir,Sujyoti Chandra,Chulhwan S Kwak,Yann Le Guen,Pawan Nandakishore,Chung-Han Hsieh,Stefano E Rensi,Russ B Altman,Michael D Greicius,Liang Feng,Xinnan Wang

Shock (Augusta, Ga.) 60:698-706 PubMed37695737

2023

SEPSIS LEADS TO IMPAIRED MITOCHONDRIAL CALCIUM UPTAKE AND SKELETAL MUSCLE WEAKNESS BY REDUCING THE MICU1:MCU PROTEIN RATIO.

Applications

Unspecified application

Species

Unspecified reactive species

Xuexin Li,Bowen Sun,Jie Li,Wanlin Ye,Mingjuan Li,Fasheng Guan,Songlin Wu,Xuerong Luo,Jianguo Feng,Jing Jia,Xueru Liu,Tao Li,Li Liu

Cardiovascular diabetology 22:216 PubMed37592255

2023

Endothelial MICU1 alleviates diabetic cardiomyopathy by attenuating nitrative stress-mediated cardiac microvascular injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xide Shi,Chao Liu,Jiangwei Chen,Shiqiang Zhou,Yajuan Li,Xingcheng Zhao,Jinliang Xing,Junhui Xue,Fengzhou Liu,Fei Li

International journal of oncology 63: PubMed37264968

2023

Targeting the mitochondrial calcium uniporter inhibits cancer progression and alleviates cisplatin resistance in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Miao,Xiaofei Wang,Yafang Lai,Ying Huang,Hua Yin,Xiangkun Meng,Hao Liu,Ruirui Hou,Wan Lin,Xiaoxu Zhang,Xu Zhang,Bei Cho Chai,Feixiong Zhang,Le Guo,Shaoqi Yang

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MICU2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU2 antibody (AB101465)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU2 antibody (AB101465)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (46)

Complementary Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

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