Rabbit Polyclonal MICU2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 35 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MICU2 aa 50-250.
Images
Publications
Filter by
- Cardiovascular diabetology 22:2162023Endothelial MICU1 alleviates diabetic cardiomyopathy by attenuating nitrative stress-mediated cardiac microvascular injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXide Shi,Chao Liu,Jiangwei Chen,Shiqiang Zhou,Yajuan Li,Xingcheng Zhao,Jinliang Xing,Junhui Xue,Fengzhou Liu,Fei LiPubMed 37592255
- International journal of oncology 63:2023Targeting the mitochondrial calcium uniporter inhibits cancer progression and alleviates cisplatin resistance in esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Miao,Xiaofei Wang,Yafang Lai,Ying Huang,Hua Yin,Xiangkun Meng,Hao Liu,Ruirui Hou,Wan Lin,Xiaoxu Zhang,Xu Zhang,Bei Cho Chai,Feixiong Zhang,Le Guo,Shaoqi YangPubMed 37264968
- Proceedings of the National Academy of Sciences of the United States of America 120:e22189991202023MICU1 occludes the mitochondrial calcium uniporter in divalent-free conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMacarena Rodríguez-Prados,Elena Berezhnaya,Maria Teresa Castromonte,Sergio L Menezes-Filho,Melanie Paillard,György HajnóczkyPubMed 37126688
- iScience 26:1062962023Neuronal loss of NCLX-dependent mitochondrial calcium efflux mediates age-associated cognitive decline.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPooja Jadiya,Henry M Cohen,Devin W Kolmetzky,Ashlesha A Kadam,Dhanendra Tomar,John W ElrodPubMed 36936788
- Physiological reports 11:e155882023Modulation of the mitochondrial Ca uniporter complex subunit expression by different shear stress patterns in vascular endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkshar Patel,Julia G Pietromicca,Manigandan Venkatesan,Soumya Maity,Jonathan E Bard,Muniswamy Madesh,B Rita AlevriadouPubMed 36754446
- Antioxidants (Basel, Switzerland) 12:2023Mild Traumatic Brain Injury Induces Mitochondrial Calcium Overload and Triggers the Upregulation of NCLX in the Hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRodrigo G Mira,Rodrigo A Quintanilla,Waldo CerpaPubMed 36829963
- Journal of cell science 135:2022Mitochondrial Ca2+ uniporter haploinsufficiency enhances long-term potentiation at hippocampal mossy fibre synapses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael J Devine,Blanka R Szulc,Jack H Howden,Guillermo López-Doménech,Arnaud Ruiz,Josef T KittlerPubMed 36274588
- Molecular cell 82:3661-3676.e82022Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen-Wei Tsai,Madison X Rodriguez,Anna M Van Keuren,Charles B Phillips,Hannah M Shushunov,Jessica E Lee,Anastacia M Garcia,Amrut V Ambardekar,Joseph C Cleveland,Julie A Reisz,Catherine Proenza,Kathryn C Chatfield,Ming-Feng TsaiPubMed 36206740
- Cell death & disease 13:4192022Mitochondrial calcium uptake regulates tumour progression in embryonal rhabdomyosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHsin Yao Chiu,Amos Hong Pheng Loh,Reshma TanejaPubMed 35490194
- Science advances 8:eabj47162022Uncontrolled mitochondrial calcium uptake underlies the pathogenesis of neurodegeneration in MICU1-deficient mice and patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaghavendra Singh,Adam Bartok,Melanie Paillard,Ashley Tyburski,Melanie Elliott,György HajnóczkyPubMed 35302860
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MICU2 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IYU8
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains (PubMed:24503055, PubMed:24560927, PubMed:26903221, PubMed:28615291, PubMed:30699349, PubMed:31397067, PubMed:32494073, PubMed:32667285, PubMed:32762847, PubMed:32790952). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium (PubMed:24560927, PubMed:26903221, PubMed:28615291, PubMed:30699349, PubMed:31397067, PubMed:32148862, PubMed:32494073, PubMed:32667285, PubMed:32762847, PubMed:32790952). At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (PubMed:32494073, PubMed:32667285, PubMed:32762847). At higher calcium levels, calcium-binding to MICU1 and MICU2 induces a conformational change that weakens MCU-MICU1 interactions and moves the MICU1-MICU2 heterodimer away from the pore, allowing calcium permeation through the MCU channel (PubMed:32494073, PubMed:32667285, PubMed:32762847).
Alternative names
EFHA1, MICU2, hMICU3, EF-hand domain-containing family member A1
Recommended products
Rabbit Polyclonal MICU2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 35 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MICU2 aa 50-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MICU2 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IYU8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MICU2 also known as mitochondrial calcium uptake 2 is an important regulator of calcium ion influx into mitochondria. It is a 54 kDa protein that resides primarily in the inner mitochondrial membrane where it helps maintain calcium homeostasis. By modulating calcium uptake MICU2 influences various cellular processes and mitochondrial dynamics. Its expression levels vary among the tissues with notable levels observed in muscle and brain tissues.
- Biological function summary
MICU2 is a component of the mitochondrial calcium uniporter complex which includes other proteins such as MCU and MICU1. This complex ensures proper calcium ion transfer across the mitochondrial membrane which is essential for ATP production maintenance of mitochondrial membrane potential and regulation of apoptotic signaling. MICU2 works as a gatekeeper preventing calcium overload and facilitating controlled entry of calcium ions.
- Pathways
MICU2 is involved in pathways essential for cellular energy metabolism and apoptosis. It participates in the calcium signaling pathway where it functions alongside MCU to control calcium entry impacting processes like oxidative phosphorylation. Another related pathway is the intrinsic apoptotic pathway where MICU2’s regulation of calcium levels can influence mitochondrial-mediated apoptosis. In this context MICU1 acts as a supportive partner by providing threshold control for calcium uptake.
- Associated diseases and disorders
MICU2 is linked to neurodegenerative diseases and mitochondrial dysfunction disorders. Abnormalities in MICU2 expression or function can lead to improper calcium homeostasis contributing to conditions like Alzheimer's disease by affecting neuronal calcium signaling. The interaction of MICU2 with MCU and MICU1 within the uniporter complex is critical for maintaining cellular health and disruptions in these relationships may exacerbate disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-MICU2 antibody (ab101465)
10% SDS-PAGE
All lanes: Western blot - Anti-MICU2 antibody (ab101465) at 1/1000 dilution
All lanes: U87-MG whole cell lysate at 30 µg
Predicted band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU2 antibody (ab101465)
Immunohistochemical analysis of FFPE mouse kidney tissue, labeling MICU2 with ab101465 (diluted 1/500).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU2 antibody (ab101465)
Immunohistochemical analysis of FFPE human hepatoma tissue, labeling MICU2 with ab101465 (diluted 1/500).
Immunocytochemistry/ Immunofluorescence - Anti-MICU2 antibody (ab101465)
Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed MCF-7 cell line, labeling MICU2 with ab101465 (diluted 1/200). DNA stained with Hoechst 33342.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com