JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB70770

Anti-MID1 antibody

Be the first to review this product! Submit a review

|

(9 Publications)

Rabbit Polyclonal MID1 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human MID1.

View Alternative Names

FXY, RNF59, TRIM18, XPRF, MID1, E3 ubiquitin-protein ligase Midline-1, Midin, Putative transcription factor XPRF, RING finger protein 59, RING finger protein Midline-1, RING-type E3 ubiquitin transferase Midline-1, Tripartite motif-containing protein 18

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1 antibody (AB70770)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1 antibody (AB70770)

ab70770 at 1/50 dilution staining MID1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Immunocytochemistry/ Immunofluorescence - Anti-MID1 antibody (AB70770)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MID1 antibody (AB70770)

ab70770 at 1/500 dilution staining MID1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Western blot - Anti-MID1 antibody (AB70770)
  • WB

Unknown

Western blot - Anti-MID1 antibody (AB70770)

All lanes:

Western blot - Anti-MID1 antibody (ab70770) at 1/500 dilution

Lane 1:

293 extract at 30 µg

Lane 2:

293 extract at 30 µg with immunising peptide

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

ELISA, ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MID1. The exact immunogen used to generate this antibody is proprietary information.

O15344

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab70770 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MID1 protein also known as midlin or tripartite motif-containing protein 18 plays a significant role in cellular activities. Weighing around 72 kDa this protein consists of numerous domains including the B-boxes and coiled-coil regions. MID1 is usually expressed in the cytoplasm and nucleus of various cell types emphasizing its involvement in cellular processes. Its expression varies in different tissues which can influence its function across multiple biological contexts.
Biological function summary

The MID1 protein serves as an E3 ubiquitin ligase contributing to protein degradation processes. As part of the multiprotein complex MID1 controls the stability of microtubules and interacts with other cytoplasmic proteins. It regulates the turnover of the protein PP2An important for maintaining cellular balance by tagging it for degradation. This function ensures proper cellular morphology and signaling.

Pathways

MID1 influences significant cellular pathways involving the regulation of microtubule dynamics and the degradation of PP2A. It engages in pathways that include the ubiquitin-proteasome system and interacts with proteins linked to these pathways such as alpha4. The interaction with alpha4 suggests a regulatory role in cellular growth and signaling therefore affecting processes like cell cycle control.

MID1 has direct links to conditions such as X-linked Opitz G/BBB syndrome and certain cancers. The dysfunction of MID1 often due to mutations can disrupt normal developmental processes leading to Opitz G/BBB syndrome where it is associated with proteins like PP2A. Similarly alterations in MID1 activity contribute to oncogenesis through its dysregulated participation in protein degradation pathways affecting cellular homeostasis and signaling networks.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.
See full target information MID1
style
left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

BMC genomics 24:68 PubMed36759768

2023

Morphometric analysis of the size-adjusted linear dimensions of the skull landmarks revealed craniofacial dysmorphology in Mid1-cKO mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yaohui Liang,Chao Song,Jieli Li,Ting Li,Chunlei Zhang,Yi Zou

Cell death & disease 13:601 PubMed35821212

2022

Mid1 is associated with androgen-dependent axonal vulnerability of motor neurons in spinal and bulbar muscular atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Ogura,Kentaro Sahashi,Tomoki Hirunagi,Madoka Iida,Takaki Miyata,Masahisa Katsuno

Frontiers in physiology 12:709506 PubMed34434118

2021

TRIM18-Regulated STAT3 Signaling Pathway PTP1B Promotes Renal Epithelial-Mesenchymal Transition, Inflammation, and Fibrosis in Diabetic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Chen,Chan Gao,Ming Wang,Xiao Fei,Ning Zhao

Immunology 163:278-292 PubMed33513265

2021

Ubiquitin E3 ligase MID1 inhibits the innate immune response by ubiquitinating IRF3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangjie Chen,Ying Xu,Wenhui Tu,Fan Huang,Yibo Zuo,Hong-Guang Zhang,Lincong Jin,Qian Feng,Tengfei Ren,Jiuyi He,Ying Miao,Yukang Yuan,Qian Zhao,Jiapeng Liu,Renxia Zhang,Li Zhu,Feng Qian,Chuanwu Zhu,Hui Zheng,Jun Wang

Annals of translational medicine 8:1021 PubMed32953821

2020

MID1 and MID2 regulate cell migration and epithelial-mesenchymal transition via modulating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Qiao,Yuan Zhou,Chao Song,Xin Zhang,Yi Zou

Scientific reports 7:13753 PubMed29062069

2017

Resveratrol induces dephosphorylation of Tau by interfering with the MID1-PP2A complex.

Applications

Unspecified application

Species

Unspecified reactive species

Susann Schweiger,Frank Matthes,Karen Posey,Eva Kickstein,Stephanie Weber,Moritz M Hettich,Sandra Pfurtscheller,Dan Ehninger,Rainer Schneider,Sybille Krauß

American journal of physiology. Lung cellular and 311:L653-63 PubMed27496895

2016

Identification of genes differentially regulated by vitamin D deficiency that alter lung pathophysiology and inflammation in allergic airways disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel E Foong,Anthony Bosco,Niamh M Troy,Shelley Gorman,Prue H Hart,Anthony Kicic,Graeme R Zosky

Frontiers in molecular neuroscience 5:58 PubMed22563308

2012

Reduced Mid1 Expression and Delayed Neuromotor Development in daDREAM Transgenic Mice.

Applications

WB

Species

Mouse

Mara Dierssen,Laura Fedrizzi,Rosa Gomez-Villafuertes,María Martinez de Lagran,Alfonso Gutierrez-Adan,Ignasi Sahún,Belen Pintado,Juan C Oliveros,Xose M Dopazo,Paz Gonzalez,Marisa Brini,Britt Mellström,Ernesto Carafoli,Jose R Naranjo

International journal of biological sciences 7:740-52 PubMed21698000

2011

Microcystin-LR (MCLR) induces a compensation of PP2A activity mediated by α4 protein in HEK293 cells.

Applications

WB

Species

Human

Tan Li,Pu Huang,Jing Liang,Wenyu Fu,Zonglou Guo,Lihong Xu
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB22552

Anti-Sp7 / Osterix antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

  • 5
  • 24
Primary Antibodies

AB16502

Anti-NF-kB p65 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody (AB16502)

  • 4
  • 40
Primary Antibodies

AB40676

Anti-NAK/TBK1 antibody [EP611Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-NAK/TBK1 antibody [EP611Y] (AB40676)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com