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Rabbit Recombinant Monoclonal MID1IP1 antibody. Carrier free. Suitable for ICC, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MID1IP1 aa 1 to C-terminus.

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Images

Immunocytochemistry - Anti-MID1IP1 antibody [005] - BSA and Azide free (AB276070), expandable thumbnail
  • Western blot - Anti-MID1IP1 antibody [005] - BSA and Azide free (AB276070), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human MID1IP1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPA3

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/20.00000 - 1/100.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/1000.00000
Notes

-

Target data

Function

Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules (By similarity).

Alternative names

MIG12, MID1IP1, Mid1-interacting protein 1, Gastrulation-specific G12-like protein, Mid1-interacting G12-like protein, Protein STRAIT11499, Spot 14-related protein, S14R, Spot 14-R

Recommended products

Rabbit Recombinant Monoclonal MID1IP1 antibody. Carrier free. Suitable for ICC, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MID1IP1 aa 1 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human MID1IP1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPA3
Carrier free
Yes
Clone number
005
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MID1IP1 also known as mid1-interacting protein 1 is an important protein involved mechanically in cell cycle regulation and ribosomal biogenesis. With a molecular mass of approximately 47 kDa it is expressed in various tissues displaying higher levels in the liver adipose tissue and skeletal muscle. The presence of MID1IP1 in multiple tissues indicates its significant role in cellular function across different biological systems.

Biological function summary

MID1IP1 plays an important role in regulating ribosomal RNA synthesis and therefore impacts protein synthesis and cellular growth. It forms a complex with MID1 another protein with well-documented functions in microtubule stabilization and regulation of glutamate dehydrogenase activity. The interaction between MID1IP1 and MID1 is essential for maintaining stability and functionality of the microtubule network which is important for correct spindle assembly during cell division.

Pathways

MID1IP1 integrates closely with mTOR signaling and the Wnt signaling pathway. These pathways are vital for cellular growth and differentiation. In the mTOR pathway MID1IP1 associates with proteins like Raptor and mLST8 aiding in the regulation of cellular growth conditions. Within the Wnt pathway though not directly an important player MID1IP1 influences the stabilization of β-catenin an important effector of Wnt signaling involved in gene transcription regulation.

Associated diseases and disorders

MID1IP1 has significant implications in metabolic disorders and cancer. Abnormal expression or function of MID1IP1 is linked to insulin resistance in metabolic diseases and it is implicated in the progression of certain cancers through its role in cell growth pathways. Additionally MID1IP1's association with proteins like p53 emphasizes its potential role in tumor suppression considering p53's well-established function in preventing cancer development.

Product promise

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2 product images

  • Immunocytochemistry - Anti-MID1IP1 antibody [005] - BSA and Azide free (ab276070), expandable thumbnail

    Immunocytochemistry - Anti-MID1IP1 antibody [005] - BSA and Azide free (ab276070)

    Immunofluorescent analysis of 4% PFA fixed, 0.1% Triton X-100 in PBS permeabilzed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling MID1IP1 with ab276070 at 1:60 dilution, 4°C overnight, followed by Goat Anti-rabbit IgG secondary antibody Alexa Fluor©488-conjugated (green). Positive staining was localized to Cytoskeleton. Cells were blocked with 10% serum prior incubation.

  • Western blot - Anti-MID1IP1 antibody [005] - BSA and Azide free (ab276070), expandable thumbnail

    Western blot - Anti-MID1IP1 antibody [005] - BSA and Azide free (ab276070)

    All lanes: Western blot - Anti-MID1IP1 antibody [005] - BSA and Azide free (ab276070) at 1/500 dilution

    All lanes: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

    Predicted band size: 20 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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