Skip to main content

Rabbit Polyclonal MID1IP1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MID1IP1 aa 50-150.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1IP1 antibody (AB224550), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1IP1 antibody (AB224550), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1IP1 antibody (AB224550), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human MID1IP1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPA3

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/50.00000 - 1/200.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal MID1IP1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MID1IP1 aa 50-150.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human MID1IP1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPA3
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MID1IP1 also known as mid1-interacting protein 1 is an important protein involved mechanically in cell cycle regulation and ribosomal biogenesis. With a molecular mass of approximately 47 kDa it is expressed in various tissues displaying higher levels in the liver adipose tissue and skeletal muscle. The presence of MID1IP1 in multiple tissues indicates its significant role in cellular function across different biological systems.

Biological function summary

MID1IP1 plays an important role in regulating ribosomal RNA synthesis and therefore impacts protein synthesis and cellular growth. It forms a complex with MID1 another protein with well-documented functions in microtubule stabilization and regulation of glutamate dehydrogenase activity. The interaction between MID1IP1 and MID1 is essential for maintaining stability and functionality of the microtubule network which is important for correct spindle assembly during cell division.

Pathways

MID1IP1 integrates closely with mTOR signaling and the Wnt signaling pathway. These pathways are vital for cellular growth and differentiation. In the mTOR pathway MID1IP1 associates with proteins like Raptor and mLST8 aiding in the regulation of cellular growth conditions. Within the Wnt pathway though not directly an important player MID1IP1 influences the stabilization of β-catenin an important effector of Wnt signaling involved in gene transcription regulation.

Associated diseases and disorders

MID1IP1 has significant implications in metabolic disorders and cancer. Abnormal expression or function of MID1IP1 is linked to insulin resistance in metabolic diseases and it is implicated in the progression of certain cancers through its role in cell growth pathways. Additionally MID1IP1's association with proteins like p53 emphasizes its potential role in tumor suppression considering p53's well-established function in preventing cancer development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1IP1 antibody (ab224550), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1IP1 antibody (ab224550)

    Paraffin-embedded human testis tissue stained for MID1IP1 using ab224550 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1IP1 antibody (ab224550), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1IP1 antibody (ab224550)

    Paraffin-embedded human skeletal muscle tissue stained for MID1IP1 using ab224550 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1IP1 antibody (ab224550), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1IP1 antibody (ab224550)

    Paraffin-embedded human stomach tissue stained for MID1IP1 using ab224550 at 1/50 dilution in immunohistochemical analysis.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com