Name: Anti-Midkine antibody [EP1143Y]
SKU: ab52637
Rating: 4 (1 reviews)

Anti-Midkine antibody [EP1143Y] (ab52637) is a rabbit monoclonal antibody that is used to detect Midkine in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, samples.

- Specificity confirmed with Midkine knockout cell line validation

Related conjugates and formulations

ab236781
Carrier free
Anti-Midkine antibody [EP1143Y] - BSA and Azide free
ab305770
Conjugated
PE Anti-Midkine antibody [EP1143Y]
ab305771
Conjugated
APC Anti-Midkine antibody [EP1143Y]
ab305772
Conjugated
HRP Anti-Midkine antibody [EP1143Y]
ab310439
Conjugated
Alexa Fluor® 594 Anti-Midkine antibody [EP1143Y]
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Conjugated
Alexa Fluor® 488 Anti-Midkine antibody [EP1143Y]
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Conjugated
Alexa Fluor® 647 Anti-Midkine antibody [EP1143Y]
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Conjugated
Alexa Fluor® 555 Anti-Midkine antibody [EP1143Y]
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Conjugated
Alexa Fluor® 568 Anti-Midkine antibody [EP1143Y]
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Conjugated
Alexa Fluor® 750 Anti-Midkine antibody [EP1143Y]

Images

Immunoprecipitation - Anti-Midkine antibody [EP1143Y] (AB52637), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested
Synthetic peptide - Human	Not recommended	Not recommended	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/20	Notes For unpurified use at 1/30.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info 1/1000	Notes For unpurified use at 1/500.
Species Human	Dilution info 1/1000	Notes For unpurified use at 1/500.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes For unpurified use at 1/250 - 1/500.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Target data

See full target information MDK

Function

Secreted protein that functions as a cytokine and growth factor and mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors (PubMed:10212223, PubMed:10772929, PubMed:12084985, PubMed:12122009, PubMed:12573468, PubMed:15466886, PubMed:18469519, PubMed:24458438). Binds cell-surface proteoglycan receptors via their chondroitin sulfate (CS) groups (PubMed:10212223, PubMed:12084985). Thereby regulates many processes like inflammatory response, cell proliferation, cell adhesion, cell growth, cell survival, tissue regeneration, cell differentiation and cell migration (PubMed:10212223, PubMed:10683378, PubMed:10772929, PubMed:12084985, PubMed:12122009, PubMed:12573468, PubMed:15466886, PubMed:22323540, PubMed:24458438). Participates in inflammatory processes by exerting two different activities. Firstly, mediates neutrophils and macrophages recruitment to the sites of inflammation both by direct action by cooperating namely with ITGB2 via LRP1 and by inducing chemokine expression (PubMed:10683378, PubMed:24458438). This inflammation can be accompanied by epithelial cell survival and smooth muscle cell migration after renal and vessel damage, respectively (PubMed:10683378). Secondly, suppresses the development of tolerogenic dendric cells thereby inhibiting the differentiation of regulatory T cells and also promote T cell expansion through NFAT signaling and Th1 cell differentiation (PubMed:22323540). Promotes tissue regeneration after injury or trauma. After heart damage negatively regulates the recruitment of inflammatory cells and mediates cell survival through activation of anti-apoptotic signaling pathways via MAPKs and AKT pathways through the activation of angiogenesis (By similarity). Also facilitates liver regeneration as well as bone repair by recruiting macrophage at trauma site and by promoting cartilage development by facilitating chondrocyte differentiation (By similarity). Plays a role in brain by promoting neural precursor cells survival and growth through interaction with heparan sulfate proteoglycans (By similarity). Binds PTPRZ1 and promotes neuronal migration and embryonic neurons survival (PubMed:10212223). Binds SDC3 or GPC2 and mediates neurite outgrowth and cell adhesion (PubMed:12084985, PubMed:1768439). Binds chondroitin sulfate E and heparin leading to inhibition of neuronal cell adhesion induced by binding with GPC2 (PubMed:12084985). Binds CSPG5 and promotes elongation of oligodendroglial precursor-like cells (By similarity). Also binds ITGA6:ITGB1 complex; this interaction mediates MDK-induced neurite outgrowth (PubMed:15466886, PubMed:1768439). Binds LRP1; promotes neuronal survival (PubMed:10772929). Binds ITGA4:ITGB1 complex; this interaction mediates MDK-induced osteoblast cells migration through PXN phosphorylation (PubMed:15466886). Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation (PubMed:12122009). Promotes epithelial to mesenchymal transition through interaction with NOTCH2 (PubMed:18469519). During arteriogenesis, plays a role in vascular endothelial cell proliferation by inducing VEGFA expression and release which in turn induces nitric oxide synthase expression. Moreover activates vasodilation through nitric oxide synthase activation (By similarity). Negatively regulates bone formation in response to mechanical load by inhibiting Wnt/beta-catenin signaling in osteoblasts (By similarity). In addition plays a role in hippocampal development, working memory, auditory response, early fetal adrenal gland development and the female reproductive system (By similarity).

Alternative names

MK1, NEGF2, MDK, Midkine, MK, Amphiregulin-associated protein, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein, ARAP

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP1143Y
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Notes

What is this antibody validated in?

Anti-Midkine antibody [EP1143Y] (ab52637) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, samples.

What is the molecular weight of Midkine?

Anti-Midkine [EP1143Y] (ab52637) specifically detects a band for Midkine (UniProt: P21741) at a molecular weight of 16kDa.

Trusted by the scientific community

Anti-Midkine [EP1143Y] (ab52637) was first used in a scientific publication in 2007 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed

The specificity of Anti-Midkine antibody [EP1143Y] (ab52637) has been confirmed by Western blot testing in MDK Knockout HAP1 cells.

Other related products

We have a range of other formats of antibody clone [EP1143Y] also available for your convenience:
ab52637, Carrier free - ab236781, PE - ab305770, APC - ab305771, HRP - ab305772, Alkaline Phosphatase - ab308722, Alexa Fluor® 488 - ab309685, Alexa Fluor® 647 - ab310049, Alexa Fluor® 594 - ab310439, Alexa Fluor® 555 - ab311967, Alexa Fluor® 568 - ab312439, Alexa Fluor® 750 - ab321660

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunoprecipitation - Anti-Midkine antibody [EP1143Y] (ab52637)
ab52637 (purified) at 1:20 dilution (2ug) immunoprecipitating Midkine in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate.
Lane 1 (input): SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug
Lane 2 (+): ab52637 & SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52637 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:5000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Midkine antibody [EP1143Y] (ab52637)
Predicted band size: 16 kDa
Observed band size: 16 kDa
Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
ab52637 was shown to react with MDK in wild-type HAP1 cells in Western blot with loss of signal observed in a MDK knockout cell line. Wild-type HAP1 and MDK knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab52637 overnight at 4 °C at a 1/500 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
Predicted band size: 16 kDa
Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
All lanes: Western blot - Anti-Midkine antibody [EP1143Y] (ab52637) at 1/500 dilution
All lanes: Recombinant protein at 0.001 µg
Secondary
All lanes: Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Midkine antibody [EP1143Y] (ab52637)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Midkine with purified ab52637 at 1:100 dilution (10.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreatic carcinoma tissue sections labeling Midkine with purified ab52637 at 1:50 dilution (2.1 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
All lanes: Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
Predicted band size: 16 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EP1143Y] (ab52637)
Human liver carcinoma stained with unpurified ab52637 at 1/100 - 1/250 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
Blocking and diluting: 5% NFDM/TBST
All lanes: Western blot - Anti-Midkine antibody [EP1143Y] (ab52637) at 1/1000 dilution
All lanes: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Midkine antibody [EP1143Y] (ab52637) at 1/10000 dilution
All lanes: Western blot - Recombinant Human Midkine protein (Recombinant Human Midkine protein ab49848) at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Midkine antibody [EP1143Y] (ab52637)
HeLa cells stained with unpurified ab52637 at 1/250 - 1/500 dilution
Flow Cytometry (Intracellular) - Anti-Midkine antibody [EP1143Y] (ab52637)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Midkine with purified ab52637 at 1/250 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.