JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB101350

Anti-MIEF2 antibody

3

(1 Review)

|

(2 Publications)

Rabbit Polyclonal MIEF2 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MIEF2 aa 250-450.

View Alternative Names

MID49, SMCR7, MIEF2, Mitochondrial dynamics protein MID49, Mitochondrial dynamics protein of 49 kDa, Mitochondrial elongation factor 2, Smith-Magenis syndrome chromosomal region candidate gene 7 protein

2 Images
Western blot - Anti-MIEF2 antibody (AB101350)
  • WB

Unknown

Western blot - Anti-MIEF2 antibody (AB101350)

10% SDS PAGE

All lanes:

Western blot - Anti-MIEF2 antibody (ab101350) at 1/500 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 49 kDa

false

Western blot - Anti-MIEF2 antibody (AB101350)
  • WB

Supplier Data

Western blot - Anti-MIEF2 antibody (AB101350)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-MIEF2 antibody (ab101350) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human MIEF2 aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

Q96C03

style
left-column

Complementary Products

Primary Antibodies

AB184247

Anti-DRP1 antibody [EPR19274]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-DRP1 antibody [EPR19274] (AB184247)

  • 5
  • 5
Primary Antibodies

AB186735

Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)

  • 5
  • 12
Primary Antibodies

AB124773

Anti-Mitofusin 2 antibody [NIAR164]

KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (AB124773)

  • 4
  • 6
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mitochondrial elongation factor 2 (MIEF2) also known as SMCR7L plays a critical role in regulating mitochondrial dynamics particularly in the process of mitochondrial fusion. This protein with a molecular weight of approximately 48 kDa helps control the balance between mitochondrial fission and fusion which is essential for maintaining mitochondrial function and cellular homeostasis. MIEF2 is expressed in various tissues with pronounced expression in the heart brain and skeletal muscle reflecting its importance in high-energy-demand tissues.
Biological function summary

The regulation of mitochondrial dynamics through MIEF2 involves the modulation of mitofusin proteins which are integral to the fusion process. MIEF2 forms a part of large protein complexes that interact with mitofusin 2 (MFN2) to promote mitochondrial outer membrane fusion. This regulatory activity helps maintain the structural integrity of mitochondrian important for energy production and apoptosis regulation.

Pathways

MIEF2 plays a pivotal role within mitochondrial quality control pathways which are essential for cellular homeostasis. MIEF2's interaction with MFN2 is integral to the mitophagy pathway a specialized form of autophagy that removes damaged mitochondria. Additionally the coordination between MIEF2 and dynamin-related protein 1 (DRP1) has significant implications on the balance between fusion and fission processes therefore influencing the overall dynamics of mitochondrial networks.

MIEF2 shows connection to neurodegenerative diseases and cardiovascular disorders. Its role in mitochondrial dynamics makes it relevant in conditions such as Alzheimer's disease where mitochondrial dysfunction is observed. MIEF2's interaction with MFN2 is especially important in Charcot-Marie-Tooth disease type 2A a disorder affecting peripheral nerves. Understanding the dysregulation of MIEF2 can provide insights into the pathophysiology of these disorders highlighting its potential as a target for therapeutic intervention.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Mitochondrial outer membrane protein involved in the regulation of mitochondrial organization (PubMed : 29361167). It is required for mitochondrial fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity.
See full target information MIEF2
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biology of the cell 114:309-319 PubMed35924634

2022

CMT2A-linked mitochondrial hyperfusion-driving mutant MFN2 perturbs ER-mitochondrial associations and Ca homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rajdeep Das,Subhrangshu Das,Saikat Chakrabarti,Oishee Chakrabarti

Redox biology 20:261-274 PubMed30384260

2018

Mst1 deletion attenuates renal ischaemia-reperfusion injury: The role of microtubule cytoskeleton dynamics, mitochondrial fission and the GSK3β-p53 signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Li,Jianxun Feng,Yunfang Zhang,Junxia Feng,Qi Wang,Shili Zhao,Ping Meng,Jingchun Li
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com