Rabbit Polyclonal MIEF2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MIEF2 aa 250-450.
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- Biology of the cell 114:309-3192022CMT2A-linked mitochondrial hyperfusion-driving mutant MFN2 perturbs ER-mitochondrial associations and Ca homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRajdeep Das,Subhrangshu Das,Saikat Chakrabarti,Oishee ChakrabartiPubMed 35924634
- Redox biology 20:261-2742018Mst1 deletion attenuates renal ischaemia-reperfusion injury: The role of microtubule cytoskeleton dynamics, mitochondrial fission and the GSK3β-p53 signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Li,Jianxun Feng,Yunfang Zhang,Junxia Feng,Qi Wang,Shili Zhao,Ping Meng,Jingchun LiPubMed 30384260
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MIEF2 aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q96C03
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Target data
Function
Mitochondrial outer membrane protein involved in the regulation of mitochondrial organization (PubMed:29361167). It is required for mitochondrial fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity.
Alternative names
MID49, SMCR7, MIEF2, Mitochondrial dynamics protein MID49, Mitochondrial dynamics protein of 49 kDa, Mitochondrial elongation factor 2, Smith-Magenis syndrome chromosomal region candidate gene 7 protein
Recommended products
Rabbit Polyclonal MIEF2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MIEF2 aa 250-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MIEF2 aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q96C03
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The mitochondrial elongation factor 2 (MIEF2) also known as SMCR7L plays a critical role in regulating mitochondrial dynamics particularly in the process of mitochondrial fusion. This protein with a molecular weight of approximately 48 kDa helps control the balance between mitochondrial fission and fusion which is essential for maintaining mitochondrial function and cellular homeostasis. MIEF2 is expressed in various tissues with pronounced expression in the heart brain and skeletal muscle reflecting its importance in high-energy-demand tissues.
- Biological function summary
The regulation of mitochondrial dynamics through MIEF2 involves the modulation of mitofusin proteins which are integral to the fusion process. MIEF2 forms a part of large protein complexes that interact with mitofusin 2 (MFN2) to promote mitochondrial outer membrane fusion. This regulatory activity helps maintain the structural integrity of mitochondrian important for energy production and apoptosis regulation.
- Pathways
MIEF2 plays a pivotal role within mitochondrial quality control pathways which are essential for cellular homeostasis. MIEF2's interaction with MFN2 is integral to the mitophagy pathway a specialized form of autophagy that removes damaged mitochondria. Additionally the coordination between MIEF2 and dynamin-related protein 1 (DRP1) has significant implications on the balance between fusion and fission processes therefore influencing the overall dynamics of mitochondrial networks.
- Associated diseases and disorders
MIEF2 shows connection to neurodegenerative diseases and cardiovascular disorders. Its role in mitochondrial dynamics makes it relevant in conditions such as Alzheimer's disease where mitochondrial dysfunction is observed. MIEF2's interaction with MFN2 is especially important in Charcot-Marie-Tooth disease type 2A a disorder affecting peripheral nerves. Understanding the dysregulation of MIEF2 can provide insights into the pathophysiology of these disorders highlighting its potential as a target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-MIEF2 antibody (ab101350)
10% SDS PAGE
All lanes: Western blot - Anti-MIEF2 antibody (ab101350) at 1/500 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 49 kDa
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