AB55445

Anti-MIF antibody [2A10-4D3]

Name: Anti-MIF antibody [2A10-4D3] (ab55445) | Abcam
Brand: Abcam Limited
SKU: AB55445
Rating: 5 (2 reviews)

(2 Reviews)

(17 Publications)

Mouse Monoclonal MIF antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 17 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MIF.

View Alternative Names

GLIF, MMIF, MIF, Macrophage migration inhibitory factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, GIF

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [2A10-4D3] (AB55445)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [2A10-4D3] (AB55445)

Paraffin embedded human lung squamous cell carcinoma tissue stained for MIF using ab55445 at 6 μg/ml in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [2A10-4D3] (AB55445)

MIF antibody (ab55445) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lung, adenosqumous cell carcinoma tissue.

Flow Cytometry - Anti-MIF antibody [2A10-4D3] (AB55445)

Flow Cyt

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Flow Cytometry - Anti-MIF antibody [2A10-4D3] (AB55445)

Overlay histogram showing Jurkat cells stained with ab55445 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55445, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [2A10-4D3] (AB55445)

Immunohistochemical analysis of Human nasopharyngeal carcinoma, staining MIF with ab55445 at 1/100 dilution.

This image was generated using the ascites version of the product.

Image from Li J et al., J Biol Chem. 2012 Oct 12;287(42):35484-95. doi: 10.1074/jbc.M112.367532. Epub 2012 Aug 14.Fig 7.; October 12, 2012 The Journal of Biological Chemistry, 287, 35484-35495.

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Western blot - Anti-MIF antibody [2A10-4D3] (AB55445)

Western blot against tagged recombinant protein immunogen using ab55445 MIF antibody at 1μg/ml. Predicted band size of immunogen is 38 kDa

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-MIF antibody [2A10-4D3] (ab55445)

Predicted band size: 12 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2A10-4D3

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MIF.

P14174

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.

Biological function summary

MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.

Pathways

MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.

MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). Counteracts the anti-inflammatory activity of glucocorticoids (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed : 11439086, PubMed : 17526494). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed : 11439086, PubMed : 17526494).

See full target information MIF

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:751 PubMed37880655

2023

Decoding meningioma heterogeneity and neoplastic cell-macrophage interaction through single-cell transcriptome profiling across pathological grades.

Applications

Unspecified application

Species

Unspecified reactive species

Hailang Fan,Lairong Song,Jian Fan,Junpeng Ma,Xiaojie Li,Junting Zhang,Jian Hu,Zhen Wu,Dake Zhang,Liang Wang

Medicine 99:e23277 PubMed33327252

2020

Prognostic role of macrophage migration inhibitory factor in patients with clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo Jung An,Hyun Min Koh,Jong Sil Lee,Dae Hyun Song

Thoracic cancer 10:2209-2217 PubMed31602798

2019

Prognostic role of macrophage migration inhibitory factor expression in patients with squamous cell carcinoma of the lung.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Min Koh,Dong Chul Kim,Yu-Min Kim,Dae Hyun Song

Molecular carcinogenesis 58:898-912 PubMed30667094

2019

MIF promotes perineural invasion through EMT in salivary adenoid cystic carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Zhang,Zhu-Feng Li,Hao-Fan Wang,Sha-Sha Wang,Xiang-Hua Yu,Jing-Biao Wu,Xin Pang,Jia-Shun Wu,Xiao Yang,Ya-Jie Tang,Li Li,Xin-Hua Liang,Min Zheng,Ya-Ling Tang

Pathology, research and practice 214:1192-1198 PubMed30041958

2018

Macrophage migration inhibitory factor is a novel prognostic marker for human oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Kang,Ying Zhang,Yan Sun

International journal of clinical and experimental 10:10066-10074 PubMed31966897

2017

Expression of STING and MIF in tumor infiltration lymphocytes as prognostic factors in patients with ESCC.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Chao Wang,Lin Zhang,Ze-Lei Li,Jing He,Ting-Ting Cai,Da-Jun Yang,De-Rong Xie

Scientific reports 7:42874 PubMed28230058

2017

Macrophage Migration Inhibitory Factor is subjected to glucose modification and oxidation in Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Omar Kassaar,Marta Pereira Morais,Suying Xu,Emily L Adam,Rosemary C Chamberlain,Bryony Jenkins,Tony D James,Paul T Francis,Stephen Ward,Robert J Williams,Jean van den Elsen

Tumour biology : the journal of the International 37:13205-13214 PubMed27456360

2016

MicroRNA-497 downregulation contributes to cell proliferation, migration, and invasion of estrogen receptor alpha negative breast cancer by targeting estrogen-related receptor alpha.

Applications

Unspecified application

Species

Unspecified reactive species

Li Han,Bo Liu,Lixi Jiang,Junyan Liu,Shumei Han

Molecular medicine reports 13:174-80 PubMed26549761

2015

Transient transfection of macrophage migration inhibitory factor small interfering RNA disrupts the biological behavior of oral squamous carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zeng,Jingjing Quan,Xuefeng Xia

Endocrine-related cancer 22:179-90 PubMed25600560

2015

CD74 expression and its therapeutic potential in thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Ping Cheng,Chien-Liang Liu,Ming-Jen Chen,Ming-Nan Chien,Ching-Hsiang Leung,Chi-Hsin Lin,Yi-Chiung Hsu,Jie-Jen Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MIF antibody [2A10-4D3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [2A10-4D3] (AB55445)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [2A10-4D3] (AB55445)

Flow Cytometry - Anti-MIF antibody [2A10-4D3] (AB55445)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [2A10-4D3] (AB55445)

Western blot - Anti-MIF antibody [2A10-4D3] (AB55445)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise