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AB65869

Anti-MIF antibody

5

(4 Reviews)

|

(13 Publications)

Rabbit Polyclonal MIF antibody. Suitable for WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human MIF.

View Alternative Names

GLIF, MMIF, MIF, Macrophage migration inhibitory factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, GIF

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody (AB65869)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody (AB65869)

MIF was detected in paraffin-embedded section of human mammary cnacer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65869 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody (AB65869)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody (AB65869)

MIF was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65869 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

Western blot - Anti-MIF antibody (AB65869)
  • WB

Supplier Data

Western blot - Anti-MIF antibody (AB65869)

All lanes:

Western blot - Anti-MIF antibody (ab65869)

Lane 1:

HeLa cell lysate

Lane 2:

COLO320 cell lysate

Lane 3:

MM231 cell lysate

Predicted band size: 12 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MIF. The exact immunogen used to generate this antibody is proprietary information.

P14174

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 0.2mL
Purification technique
Affinity purification Immunogen
Storage buffer
Constituents: 76.92% Trehalose, 17.31% Sodium chloride, 3.85% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.
Biological function summary

MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.

Pathways

MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.

MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). Counteracts the anti-inflammatory activity of glucocorticoids (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed : 11439086, PubMed : 17526494). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed : 11439086, PubMed : 17526494).
See full target information MIF
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 22:e3002194 PubMed39495793

2024

Apoptotic extracellular vesicles carrying Mif regulate macrophage recruitment and compensatory proliferation in neighboring epithelial stem cells during tissue maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Safia A Essien,Ivanshi Ahuja,George T Eisenhoffer

Heliyon 10:e23635 PubMed38187273

2024

High species homology potentiates quantitative inflammation profiling in zebrafish using immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

T Ollewagen,R M Benecke,C Smith

Journal of neurology, neurosurgery, and psychiatry 94:550-559 PubMed37012067

2023

Molecules of senescent glial cells differentiate Alzheimer's disease from ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Linbin Dai,Feng Gao,Qiong Wang,Xinyi Lv,Zhaozhao Cheng,Yan Wu,Xianliang Chai,Henrik Zetterberg,Kaj Blennow,Allan I Levey,Jiong Shi,Yong Shen

Frontiers in oncology 12:1028070 PubMed36703790

2023

MIF promotes cell invasion by the LRP1-uPAR interaction in pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huizhi Sun,Runfen Cheng,Danfang Zhang,Yuhong Guo,Fan Li,Yanlei Li,Yue Li,Xiaoyu Bai,Jing Mo,Chongbiao Huang

BMC anesthesiology 22:289 PubMed36104681

2022

Dexmedetomidine reduces myocardial ischemia-reperfusion injury in young mice through MIF/AMPK/GLUT4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Siyu Chen,Aimei Li,Jianjiang Wu,Yidan Huang,Tiantian Zou,Taiwangu Tailaiti,Jiang Wang

Frontiers in oncology 12:810526 PubMed35311093

2022

Construction of a Novel Ferroptosis-Related Gene Signature for Predicting Survival of Patients With Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojie Song,Liqun Wu,Guangqiang Wang,Baoyi Liu,Wenyong Zhu

Physiological reports 9:e15119 PubMed34806343

2021

Unresolved intramuscular inflammation, not diminished skeletal muscle regenerative capacity, is at the root of rheumatoid cachexia: insights from a rat CIA model.

Applications

Unspecified application

Species

Unspecified reactive species

Tracey Ollewagen,Yigael S L Powrie,Kathryn H Myburgh,Carine Smith

Technology in cancer research & treatment 19:1533033820935773 PubMed32578505

2020

Combination of Plasma MIF and VCA-IgA Improves the Diagnostic Specificity for Patients With Nasopharyngeal Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Xue,Shan Xing,Weiguo Ma,Jiahe Sheng,Zhiliang Huang,Qingxia Xu

Frontiers in immunology 10:2752 PubMed31866994

2019

The Proinflammatory and Proangiogenic Macrophage Migration Inhibitory Factor Is a Potential Regulator in Proliferative Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Abu El-Asrar,Ajmal Ahmad,Mohammad Mairaj Siddiquei,Alexandra De Zutter,Eef Allegaert,Priscilla W Gikandi,Gert De Hertogh,Jo Van Damme,Ghislain Opdenakker,Sofie Struyf

Neuroscience 416:50-62 PubMed31170483

2019

Macrophage Migration Inhibitory Factor Mediates Neuroprotective Effects by Regulating Inflammation, Apoptosis and Autophagy in Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Siying Li,Kun Nie,Qingxi Zhang,Manli Guo,Yihui Qiu,You Li,Yuyuan Gao,Lijuan Wang
View all publications
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