Anti-MIF antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody (AB7207)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MIF with ab322733 at 1/500 (1.016 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human kidney in (A) ab322733 and (B) ab7207.
The image on (A) ab322733 and (B) ab7207 are treated with Anti-MIF antibody at 1/500 dilution. The section was incubated with ab322733 or ab7207 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody (AB7207)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling MIF with ab322733 at 1/500 (1.016 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat kidney in (A) ab322733 and (B) ab7207.
The image on (A) ab322733 and (B) ab7207 are treated with Anti-MIF antibody at 1/500 dilution. The section was incubated with ab322733 or ab7207 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody (AB7207)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MIF with ab322733 at 1/500 (1.016 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse kidney in (A) ab322733 and (B) ab7207.
The image on (A) ab322733 and (B) ab7207 are treated with Anti-MIF antibody at 1/500 dilution. The section was incubated with ab322733 or ab7207 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-MIF antibody (AB7207)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab322733 and ab7207 were shown to bind specifically to MIF. Target of interest was observed at 13 kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in MIF knockout cell line (lane 2).

Recombinant multiclonal ab322733 showed higher sensitivity than polyclonal ab7207.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MIF antibody [RM1231] (<a href='/en-us/products/primary-antibodies/mif-antibody-rm1231-ab322733'>ab322733</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 2:

MIF knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 5:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 7:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Lane 8:

NR8383 (rat alveolar macrophage) whole cell lysate at 20 µg

Lane 9:

J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 13 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-MIF antibody (AB7207)

MIF detected by Western Blot in LPS stimulated RAW cell lysate.

All lanes:

Western blot - Anti-MIF antibody (ab7207)

All lanes:

LPS stimulated RAW cell lysate

Predicted band size: 12 kDa

false

• WB

Supplier Data

Western blot - Anti-MIF antibody (AB7207)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Recombinant multiclonal ab322733 showed higher sensitivity than polyclonal ab7207.

All lanes:

Western blot - Anti-MIF antibody [RM1231] (<a href='/en-us/products/primary-antibodies/mif-antibody-rm1231-ab322733'>ab322733</a>) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 13 kDa

false

Exposure time: 48s

• WB

Unknown

Western blot - Anti-MIF antibody (AB7207)

From a review by Arsi Rosengren.

All lanes:

Western blot - Anti-MIF antibody (ab7207)

Predicted band size: 12 kDa

false