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Rabbit Polyclonal MIF antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr, ELISA and reacts with Mouse, Rat samples. Cited in 38 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 6.5
Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Polyclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFIHC-FrELISA
Mouse
Expected
Tested
Expected
Expected
Expected
Predicted
Rat
Expected
Predicted
Expected
Expected
Expected
Expected

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Target data

Function

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity).

Alternative names

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Rabbit Polyclonal MIF antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr, ELISA and reacts with Mouse, Rat samples. Cited in 38 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.

Activity summary

Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.

Pathways

MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.

Associated diseases and disorders

MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.

Product promise

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2 product images

  • Western blot - Anti-MIF antibody (ab7207), expandable thumbnail

    Western blot - Anti-MIF antibody (ab7207)

    MIF detected by Western
    Blot in LPS stimulated
    RAW cell lysate.

    All lanes: Western blot - Anti-MIF antibody (AB7207)

    All lanes: LPS stimulated RAW cell lysate

    Performed under reducing conditions.

    Predicted band size: 12 kDa

  • Western blot - Anti-MIF antibody (ab7207), expandable thumbnail

    Western blot - Anti-MIF antibody (ab7207)

    From a review by Arsi Rosengren.

    All lanes: Western blot - Anti-MIF antibody (AB7207)

    Predicted band size: 12 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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