Rabbit Recombinant Monoclonal MIF antibody. Suitable for Flow Cyt, ICC, IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MIF aa 1 to C-terminus.
Images
![Flow Cytometry - Anti-MIF antibody [BLR141J] - BSA free (AB314057), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-blr141j-bsa-free-ab314057--flow-cytometry-img428836.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-MIF antibody [BLR141J] - BSA free (AB314057)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [BLR141J] - BSA free (AB314057), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-blr141j-bsa-free-ab314057--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420429.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [BLR141J] - BSA free (AB314057)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [BLR141J] - BSA free (AB314057), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-blr141j-bsa-free-ab314057--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420428.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [BLR141J] - BSA free (AB314057)")
![Immunocytochemistry - Anti-MIF antibody [BLR141J] - BSA free (AB314057), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-blr141j-bsa-free-ab314057--immunocytochemistry-img420427.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-MIF antibody [BLR141J] - BSA free (AB314057)")
![Western blot - Anti-MIF antibody [BLR141J] - BSA free (AB314057), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-blr141j-bsa-free-ab314057--western-blot-img420426.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MIF antibody [BLR141J] - BSA free (AB314057)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human MIF aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P14174
Reactivity data
Select an application| Flow Cyt | ICC | IHC-P | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed:15908412, PubMed:17443469, PubMed:23776208). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed:15908412, PubMed:17443469, PubMed:23776208). Counteracts the anti-inflammatory activity of glucocorticoids (PubMed:15908412, PubMed:17443469, PubMed:23776208). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed:11439086, PubMed:17526494). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed:11439086, PubMed:17526494).
Alternative names
GLIF, MMIF, MIF, Macrophage migration inhibitory factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, GIF
Recommended products
Rabbit Recombinant Monoclonal MIF antibody. Suitable for Flow Cyt, ICC, IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MIF aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human MIF aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P14174
- Clone number
- BLR141J
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.
- Biological function summary
MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.
- Pathways
MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.
- Associated diseases and disorders
MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
![Flow Cytometry - Anti-MIF antibody [BLR141J] - BSA free (ab314057), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-blr141j-bsa-free-ab314057--flow-cytometry-img428836.jpg "Flow Cytometry - Anti-MIF antibody [BLR141J] - BSA free (ab314057)")
Flow Cytometry - Anti-MIF antibody [BLR141J] - BSA free (ab314057)
Detection of human MIF (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-MIF recombinant monoclonal antibody [BLR141J] or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [BLR141J] - BSA free (ab314057), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-blr141j-bsa-free-ab314057--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420429.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [BLR141J] - BSA free (ab314057)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [BLR141J] - BSA free (ab314057)
Detection of human MIF in FFPE renal cell carcinoma by immunohistochemistry Antibody:Rabbit anti-MIF recombinant monoclonal antibody [BLR141H] (A700-141 lot 1). Secondary:HRP-conjugated goat anti-rabbit IgG Substrate:DAB.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [BLR141J] - BSA free (ab314057), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-blr141j-bsa-free-ab314057--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420428.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [BLR141J] - BSA free (ab314057)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [BLR141J] - BSA free (ab314057)
Detection of human MIF in FFPE prostate carcinoma by immunohistochemistry Antibody: Rabbit anti-MIF recombinant monoclonal antibody [BLR141H] (A700-141 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG Substrate:DAB.
![Immunocytochemistry - Anti-MIF antibody [BLR141J] - BSA free (ab314057), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-blr141j-bsa-free-ab314057--immunocytochemistry-img420427.jpg "Immunocytochemistry - Anti-MIF antibody [BLR141J] - BSA free (ab314057)")
Immunocytochemistry - Anti-MIF antibody [BLR141J] - BSA free (ab314057)
Detection of human MIF in FFPE HEK293T cells by immunocytochemistry Antibody:Rabbit anti-MIF recombinant monoclonal antibody [BLR141H] (A700-141 lot 1). Secondary:HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.
![Western blot - Anti-MIF antibody [BLR141J] - BSA free (ab314057), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-blr141j-bsa-free-ab314057--western-blot-img420426.jpg "Western blot - Anti-MIF antibody [BLR141J] - BSA free (ab314057)")
Western blot - Anti-MIF antibody [BLR141J] - BSA free (ab314057)
Samples:Whole cell lysate (25 µg) from HEK293T, HeLa, Jurkat, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-MIF recombinant monoclonal antibody [BLR141J] used at 1:1000. Secondary:H RP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).
All lanes: Western blot - Anti-MIF antibody [BLR141J] - BSA free (ab314057) at 1/1000 dilution
Lane 1: HEK293T Whole cell lysate at 25 µg
Lane 2: HeLa Whole cell lysate at 25 µg
Lane 3: Jurkat Whole cell lysate at 25 µg
Lane 4: Hep-G2 Whole cell lysate at 25 µg
Lane 5: MCF-7 Whole cell lysate at 25 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
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