Rabbit Recombinant Monoclonal MIF antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human samples.
Images
![Western blot - Anti-MIF antibody [EPR12462] - BSA and Azide free (AB249925), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-epr12462-bsa-and-azide-free-ab249925--western-blot-img6875.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MIF antibody [EPR12462] - BSA and Azide free (AB249925)")
![Immunoprecipitation - Anti-MIF antibody [EPR12462] - BSA and Azide free (AB249925), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-epr12462-bsa-and-azide-free-ab249925--immunoprecipitation-img87097.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MIF antibody [EPR12462] - BSA and Azide free (AB249925)")
![Western blot - Anti-MIF antibody [EPR12462] - BSA and Azide free (AB249925), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-epr12462-bsa-and-azide-free-ab249925--western-blot-img125664.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MIF antibody [EPR12462] - BSA and Azide free (AB249925)")
![Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR12462] - BSA and Azide free (AB249925), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-epr12462-bsa-and-azide-free-ab249925--immunocytochemistry-immunofluorescence-img441705.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR12462] - BSA and Azide free (AB249925)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
| Cow | Predicted | Predicted | Not recommended | Not recommended |
| Cynomolgus monkey | Predicted | Predicted | Not recommended | Not recommended |
| Rhesus monkey | Predicted | Predicted | Not recommended | Not recommended |
| Sheep | Predicted | Predicted | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey, Rhesus monkey, Sheep, Cow | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey, Rhesus monkey, Sheep, Cow | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cynomolgus monkey, Rhesus monkey, Sheep, Cow | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cynomolgus monkey, Rhesus monkey, Sheep, Cow, Human | Dilution info - | Notes - |
Target data
Function
Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed:15908412, PubMed:17443469, PubMed:23776208). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed:15908412, PubMed:17443469, PubMed:23776208). Counteracts the anti-inflammatory activity of glucocorticoids (PubMed:15908412, PubMed:17443469, PubMed:23776208). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed:11439086, PubMed:17526494). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed:11439086, PubMed:17526494).
Alternative names
GLIF, MMIF, MIF, Macrophage migration inhibitory factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, GIF
Recommended products
Rabbit Recombinant Monoclonal MIF antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12462
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249925 is the carrier-free version of Anti-MIF antibody [EPR12462] ab176565.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.
- Biological function summary
MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.
- Pathways
MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.
- Associated diseases and disorders
MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-MIF antibody [EPR12462] - BSA and Azide free (ab249925), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-epr12462-bsa-and-azide-free-ab249925--western-blot-img6875.jpg "Western blot - Anti-MIF antibody [EPR12462] - BSA and Azide free (ab249925)")
Western blot - Anti-MIF antibody [EPR12462] - BSA and Azide free (ab249925)
This data was developed using Anti-MIF antibody [EPR12462] ab176565, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: MIF knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: HepG2 whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-MIF antibody [EPR12462] ab176565 observed at 12 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-MIF antibody [EPR12462] ab176565 was shown to specifically recognize MIF in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when knockout cells were examined. Wild-type and MIF knockout samples were subjected to SDS-PAGE. Anti-MIF antibody [EPR12462] ab176565 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-MIF antibody [EPR12462] (Anti-MIF antibody [EPR12462] ab176565)
Predicted band size: 12 kDa
![Immunoprecipitation - Anti-MIF antibody [EPR12462] - BSA and Azide free (ab249925), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-epr12462-bsa-and-azide-free-ab249925--immunoprecipitation-img87097.jpg "Immunoprecipitation - Anti-MIF antibody [EPR12462] - BSA and Azide free (ab249925)")
Immunoprecipitation - Anti-MIF antibody [EPR12462] - BSA and Azide free (ab249925)
This data was developed using Anti-MIF antibody [EPR12462] ab176565, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate labeling MIF with Anti-MIF antibody [EPR12462] ab176565 at 1/10 dilution.
All lanes: Immunoprecipitation - Anti-MIF antibody [EPR12462] (Anti-MIF antibody [EPR12462] ab176565)
Developed using the ECL technique.
Predicted band size: 12 kDa
![Western blot - Anti-MIF antibody [EPR12462] - BSA and Azide free (ab249925), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-epr12462-bsa-and-azide-free-ab249925--western-blot-img125664.jpg "Western blot - Anti-MIF antibody [EPR12462] - BSA and Azide free (ab249925)")
Western blot - Anti-MIF antibody [EPR12462] - BSA and Azide free (ab249925)
This data was developed using Anti-MIF antibody [EPR12462] ab176565, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-MIF antibody [EPR12462] (Anti-MIF antibody [EPR12462] ab176565) at 1/1000 dilution
Lane 1: Y79 cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: A375 cell lysate at 10 µg
Lane 4: THP1 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR12462] - BSA and Azide free (ab249925), expandable thumbnail](https://content.abcam.com/products/images/mif-antibody-epr12462-bsa-and-azide-free-ab249925--immunocytochemistry-immunofluorescence-img441705.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR12462] - BSA and Azide free (ab249925)")
Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR12462] - BSA and Azide free (ab249925)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MIF antibody [EPR12462] ab176565).
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HAP1 (human chronic myelogenous leukemia near-haploid cell) cells labelling MIF with Anti-MIF antibody [EPR12462] ab176565 at 1/500 (0.976 µg/ml) dilution followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg) dilution (Green).
Confocal image showing cytoplasmic with nuclear staining in HAP1 cell line (shown in green) and no staining in MIF KO HAP1 cell line. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg) dilution.
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